Team:Osaka/week7

From 2012.igem.org

(Difference between revisions)
Line 30: Line 30:
==September 12 (Wed)==
==September 12 (Wed)==
-
 
+
# miniprep
 +
# Restriction digests(160)
 +
# Electrophoresis
 +
# Tolerance assay
 +
# Ligation(161,162)
==September 13 (Thu)==
==September 13 (Thu)==
-
 
+
# Sequencing(157,158,159,160)
 +
# Tolerance assay of 146,148
 +
# Transformation(161)
 +
# To liquid culture(144~148,162)
==September 14 (Fri)==
==September 14 (Fri)==
-
 
+
# miniprep
-
 
+
# Restriction digests(162)
 +
# Tolerance assay (Preliminary test of hydrogen peroxide)
 +
# Electrophoresis(162)
 +
# To liquid culture(161)
==September 15 (Sat)==
==September 15 (Sat)==

Revision as of 07:44, 14 September 2012


September 9 (Sun)

  1. miniprep
  2. Restriction digests(150,151,152,159,160)
  3. Electrophoresis
  4. Tolerance assay of 144,147(MMC)
  5. To liquid culture(144,145,160)


September 10 (Mon)

  1. miniprep
  2. Restriction digests(160,1-9N)
  3. Electrophoresis
  4. Preparation of LB agar plates(Amp)
  5. Ligation(160)
  6. Transformation(160)
  7. To liquid culture(146,148)
  8. Tolerance assay of 144,145(MMC)


September 11 (Tue)

  1. Restriction digests(157,158,159,160,1-9N)
  2. Electrophoresis
  3. Ligation(160)
  4. Tolerance assay of 146,148(MMC)
  5. Transformation(160)


September 12 (Wed)

  1. miniprep
  2. Restriction digests(160)
  3. Electrophoresis
  4. Tolerance assay
  5. Ligation(161,162)


September 13 (Thu)

  1. Sequencing(157,158,159,160)
  2. Tolerance assay of 146,148
  3. Transformation(161)
  4. To liquid culture(144~148,162)


September 14 (Fri)

  1. miniprep
  2. Restriction digests(162)
  3. Tolerance assay (Preliminary test of hydrogen peroxide)
  4. Electrophoresis(162)
  5. To liquid culture(161)

September 15 (Sat)

Back to Notebook

Retrieved from "http://2012.igem.org/Team:Osaka/week7"