Team:TU Darmstadt/Materials/tctA503

From 2012.igem.org

(Difference between revisions)
Line 50: Line 50:
  fwd: TGGTGCTGATGCCGGCAGTGAAGAACAAGCG
  fwd: TGGTGCTGATGCCGGCAGTGAAGAACAAGCG
  rev: CGCTTGTTCTTCACTGCCGGCATCAGCACCA
  rev: CGCTTGTTCTTCACTGCCGGCATCAGCACCA
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==== a ====
 

Revision as of 00:14, 14 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA503]

Primers for the construction of a BioBrick containing the large subunit A1 of the tripartite tricarboxylate transporter family.

tctA503 BioBrick 

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg
rev: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg

tctA503 Pst1

Primers for removing a Pst1 site. 

     5'                             3'
fwd: TGGTGCTGATGCCGGCAGTGAAGAACAAGCG
rev: CGCTTGTTCTTCACTGCCGGCATCAGCACCA
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