From 2012.igem.org

Revision as of 19:20, 13 September 2012

• Home
• Team
  • The Team
  • Team Registration
• Project
  • 1. Introduction
  • 2. PnA System
  • 3. Virus-Like Particles
    • CCMV
    • HepB
    • Polerovirus Natural BioBrick
  • 4. Outside Modification
  • 5. Inside Modification
  • 6. Detection of VLPs
  • 7. Applications
  • 8. The Constructor
  • 9. Final Overview
• Human Practices
  • Human Practices Home
  • 1. Mini Symposium
  • 2. Visiting Secondary Schools
  • 3. Discovery Festival
    • Communication Science
  • 4. Stakeholders
  • 5. Munich CAS Conference
• Safety
  • Introduction
  • 1. General safety
  • 2. Virus-related safety
  • 3. Regulations
  • 4. Safety suggestions
  • 5. Safety of applications
• Modeling
  • Human Body Model
  • Tertiary folding prediction
  • VLP assembly model
• Attributions
• Parts
• Notebook
  • Journal
  • Protocols

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Last year's project: Synchronized Oscillatory System

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week 14: 30 july - 5 august

Lab work

Bricking Hepatitis B

• Miniprepping of successful transformants from the transformation (Hep B + BBa_J04500)
• Digestion check of those minipreps

-> this digestion check was repeated 2 times (once with a longer incubation time and once with a higher amount of DNA in the digestion mixture) until the insert size could be seen on the gel

the digestion check confirmed the right insert sizes in the samples they where expected (around 800bp)

TuYV

• The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 E coli. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps!

PLRV

The PCR products obtained after Reverse Transcription followed by PCR were used as template to add iGEM prefix and suffix.

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