From 2012.igem.org

Revision as of 09:45, 18 September 2012

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• Project
  • 1. Introduction
  • 2. PnA System
  • 3. Virus-Like Particles
    • CCMV
    • HepB
    • Polerovirus Natural BioBrick
  • 4. Outside Modification
  • 5. Inside Modification
  • 6. Detection of VLPs
  • 7. Applications
  • 8. The Constructor
  • 9. Final Overview
• Human Practices
  • Human Practices Home
  • 1. Mini Symposium
  • 2. Visiting Secondary Schools
  • 3. Discovery Festival
    • Communication Science
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  • 5. Munich CAS Conference
• Safety
  • Introduction
  • 1. General safety
  • 2. Virus-related safety
  • 3. Regulations
  • 4. Safety suggestions
  • 5. Safety of applications
• Modeling
  • Human Body Model
  • Tertiary folding prediction
  • VLP assembly model
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Last year's project: Synchronized Oscillatory System

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week 11: 9 july - 15 july

Office work

Lab work

TuYV

• Two (non-readthrough) gene variants 1 and 1H were digested and ligated into pSB1A3 linearized backbone.

Written by: Han Yue

PLRV

The start of the lab work on PLRV

We went out to the potato fields near Wageningen to look for PLRV infected potatoes. We thought we found some infected plants, based on the primary symptoms namely smaller sizes of the plants and curled leafs (figure 1).

We contacted Kees Bus and Chris Cuperus, who are experts at the Wageningen University on potato diseases. Judging the pictures, they concluded that these plants were probably not infected by PLRV.

This week we started trial experiments for RNA isolation from potato plants. We used a RNA isolation protocols using Trizol (see protocol page). The RNA product concentration was very high (upto 3500 ng/µL).

RNA isolate purity checked. RNA is of very good quality. Note that smaller rRNA bands visible in the leaf sample are derived from plant organelles such as plastids and chloroplasts

HepB

• Production of a HepB PCR fragment with pre- and suffix by means of a PCR using primers annealing to a plasmid containing the Hepatitis B core protein encoding gene. These primers also have an overhang that encode for pre- and suffix

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