Team:Wageningen UR/Protocol

From 2012.igem.org

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<li>The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. [[Team:Wageningen_UR/MethodsProduction|Click here to read more]]</li>
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<li>[[Team:Wageningen_UR/MethodsProduction|Production]]</li>
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<li>After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. [[Team:Wageningen_UR/MethodsPurification|Click here to read more]]</li>
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<li>[[Team:Wageningen_UR/MethodsPurification|Purification]]</li>
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Revision as of 08:12, 14 September 2012

Contents

Methods

The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.

Protocol

Medium & Buffer recipes

CCMV Coat Protein VLP formation

Hepatitis B Coat Protein VLP formation

TuYV Coat Protein VLP formation

PLRV Coat Protein VLP formation

General Protocol

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