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Team:TU Darmstadt/Materials/EcoRI
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Latest revision as of 20:37, 26 September 2012
Contents |
EcoRI
About
EcoRI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli RY13]. It creates stick ends with 5' end overhang.
Working conditions
The recognition motive of EcoRI is:[1]
5' GAATTC --> 5'---G AATTC---3' 3' CTTAAG --> 3'---CTTAA G---5'
Buffer: NEBuffer EcoRI
EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.[2]
Supplier
- NEB http://www.neb.com/nebecomm/products/productR3101.asp
References
[1] http://en.wikipedia.org/wiki/EcoRI
[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp
