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Team:TU Darmstadt/Materials/TAE
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Latest revision as of 20:54, 26 September 2012
Contents |
TAE
About
TAE is a commonly used buffer for agarose gel electrophoresis that provides optimal resolution of fragments >4kb in length.
Application
TAE is usually prepared in 50X stocks that are diluted to 1X when needed.
Ingredients
For 1L 50x:
- 242 g Tris-HCl/Tris-base
- 57 mL 100% acetic acid
- 18,6 g EDTA (Ethylenediaminetetraacetic acid) or 100mL of 0.5M sodium EDTA
Add dH2O up to 1L
For 1L 1x:
- 20ml 50x TAE
Add dH2O up to 1L (980mL)
