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Team:TU Darmstadt/Materials/Bromphenol blue
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== Bromphenol blue == | == Bromphenol blue == | ||
=== About === | === About === | ||
| - | Bromphenol blue is a size marker used in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis agarose gel electrophoresis] | + | Bromphenol blue is a size marker used in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis agarose gel electrophoresis]<sup>[1]</sup> that does also work as a pH indicator. Below pH=3.0 it is yellow and blue-purple at more basic pH. Its specific band depends on the gel concentration:<sup>[2]</sup> |
| - | Its specific band depends on the gel concentration:<sup>[2]</sup> | + | |
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=== Application === | === Application === | ||
Revision as of 20:28, 11 September 2012
Contents |
Bromphenol blue
About
Bromphenol blue is a size marker used in agarose gel electrophoresis[1] that does also work as a pH indicator. Below pH=3.0 it is yellow and blue-purple at more basic pH. Its specific band depends on the gel concentration:[2]
| % Agarose | bp |
|---|---|
| 0,7 | 1000 |
| 1 | 500 |
| 2 | 150 |
| 3 | 50 |
Application
References
[1] http://en.wikipedia.org/wiki/Bromophenol_blue
[2] http://openwetware.org/wiki/Bromophenol_blue
