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Team:TU Darmstadt/Materials/SOB
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| - | == SOB == | + | === SOB === |
The SOB medium is used for growth of bacteria for preparing [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically competent cells]. | The SOB medium is used for growth of bacteria for preparing [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically competent cells]. | ||
| - | === Ingredients === | + | ==== Ingredients ==== |
*0.5% (w/v) yeast extract | *0.5% (w/v) yeast extract | ||
*2% (w/v) tryptone | *2% (w/v) tryptone | ||
Revision as of 23:47, 9 September 2012
SOB
The SOB medium is used for growth of bacteria for preparing chemically competent cells.
Ingredients
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
For 1 L:
- 0.186 g KCl
- 2.4 g MgSO4
- 0.584 g NaCl
- 20 g tryptone
- 5 g yeast extract
Adjust to pH 7.5 prior to use. This requires approximately 5 mL of 5M NaOH per liter.
