Team:Wageningen UR/Protocol/Towbinselectrotransferbuffer1x

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== Towbin's Electrotransfer buffer 1x==
== Towbin's Electrotransfer buffer 1x==
-
*use this buffer for Western Blotting with a nitrocellulose membrane
+
*Use this buffer for Western Blotting with a nitrocellulose membrane
ingredients:
ingredients:
*100 mL 10x stock solution
*100 mL 10x stock solution
*200 mL methanol (20%)
*200 mL methanol (20%)
-
*fill with demineralized water till 1 L
+
*Fill with demineralized water till 1 L

Latest revision as of 13:39, 24 September 2012


Towbin's Electrotransfer buffer 1x

  • Use this buffer for Western Blotting with a nitrocellulose membrane

ingredients:

  • 100 mL 10x stock solution
  • 200 mL methanol (20%)
  • Fill with demineralized water till 1 L