Team:British Columbia/NotebookKillSwitches

From 2012.igem.org

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{{Template:Team:British_Columbia_Header}}
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[http://ung.igem.org/Previous_iGEM_Competitions Previous iGEM Competitions]
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Team Abstracts: [https://2011.igem.org/Jamboree/Team_Abstracts 2011] [https://2010.igem.org/Jamboree/Project_Abstract/Team_Abstracts 2010]
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[https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts 2009]
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[https://2008.igem.org/Jamboree/Project_Abstract/Team_Abstracts 2008]
Parts Registry: [http://partsregistry.org/Cell_death Cell Death]
Parts Registry: [http://partsregistry.org/Cell_death Cell Death]
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<partinfo>BBa_I716462</partinfo> Arabinose promoter, stops reproduction, no lysis
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{| class="wikitable"
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|+ Killswitch BioBricks
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<partinfo>BBa_K512002</partinfo> Finds GFP on plasmid, eats plasmid? (Jacob says mention "crispr")
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! Numbers !! Team !! Year !! Trigger !! What it Does !! Where?
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|-
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[https://2010.igem.org/Team:ESBS-Strasbourg 2010 Strasbourg]: I don't know what the heck
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| <partinfo>BBa_I716462</partinfo> ||UC-Berkeley ||2007|| Arabinose promoter || BamHI ||On Plate 2, 3C|
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|-
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[https://2011.igem.org/Team:Tokyo_Metropolitan 2011 Tokyo Metropolitan]: Killer Bee e.coli?
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| <partinfo>BBa_K512002</partinfo> ||USC||2011|| IPTG, targets GFP gene|| CRISPR-mediated GFP plasmid degradation||
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|-
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[https://2011.igem.org/Team:UNIST_Korea 2011 UNIST Korea]: Killed by high and low temps, not if dark.
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| <partinfo>BBa_K112808</partinfo> ||UC-Berkeley ||2008 || No Promoter||T4 Lysis device || Plate 3, 6G
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|-
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[https://2010.igem.org/Team:Harvard/fences/design 2010 Harvard]: Barnase cleaves RNA!
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| <partinfo>BBa_K541545</partinfo> ||FAGEM ||2011 ||IPTG || Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible) ||
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|-
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<partinfo>BBa_K112808</partinfo>
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| <partinfo>BBa_K593009</partinfo> ||METU-Ankara||2011||T7 Promoter || ROSE regulated kill switch, (RNA thermometer, only activates above 42°) ||
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|-
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[https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010 Hong Kong]: Possible new killswitch, not implemented
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| <partinfo>BBa_K628006</partinfo> ||St. Andrews ||2011 ||pBad || Protegrin-1 Kill Switch, (Antimicrobial peptide) ||
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|-
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<partinfo>BBa_K541545</partinfo> Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
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| || Strasbourg || [https://2010.igem.org/Team:ESBS-Strasbourg 2010] || || I don't know what the heck. ||
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|-
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| || Tokyo Metropolitan || [https://2011.igem.org/Team:Tokyo_Metropolitan 2011] || || Killer Bee e.coli? ||
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| || UNIST Korea || [https://2011.igem.org/Team:UNIST_Korea 2011] || || Killed by high and low temps, not if dark. ||
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|-
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| || Harvard || [https://2010.igem.org/Team:Harvard/fences/design 2010] || || Barnase cleaves RNA! ||
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|-
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| <partinfo>BBa_I716211</partinfo>|| UC Berkeley || 2007 ||No Promoter || Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB ||
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|-
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| || Hong Kong || [https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010] || || Possible new killswitch, not implemented ||
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|-
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| <partinfo>BBa_K565001</partinfo> ||Valencia||2011|| ? 12 kb ||Colicin G
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| <partinfo>BBa_K565002</partinfo> ||Valencia ||2011|| ? 12 kb ||Colicin H
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|-
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| <partinfo>BBa_K565004</partinfo> ||Valencia ||2011 ||No Promoter ||Microcin C51 production device (5 restriction sites) ||
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| <partinfo>BBa_K185004</partinfo> ||SJTU-BioX-Shanghai ||2009 ||No Promoter ||RelE toxin+Double terminator ||
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|-
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| || UNICAMP Brazil || [https://2009.igem.org/Team:UNICAMP-Brazil/Yeastguard 2009] || || All sorts of kill switches, but may not all work in E. coli. ||
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| <partinfo>BBa_K284017</partinfo> ||UNICAMP-Brazil ||2009|| Lactate (Yeast) ||Lysozyme
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| <partinfo>BBa_K131000</partinfo> ||Calgary||2008|| SOS Promoter ||Colicin 2E||3, 22A
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|-
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| <partinfo>BBa_K112806</partinfo> ||UC Berkeley ||2008|| T7 ||T4 Endolysin||
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|-
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| <partinfo>BBa_K302035</partinfo> ||Newcastle ||2010|| Sucrose limit ||MazEF
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|-
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| <partinfo>BBa_K124014</partinfo> ||Brown ||2008 ||No Promoter|| Mutated Holin Gene (Inconsistent sequencing)||
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| <partinfo>BBa_K419003</partinfo> ||TzuChiU_Formosa ||2010 ||pLys||Lysis Cassette - no antiholin||
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| <partinfo>BBa_K317039</partinfo> ||Tokyo-NoKoGen ||2010||AHL || AHL sensitive lysis cassette ||
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| <partinfo>BBa_K317040</partinfo> ||Tokyo-NoKoGen ||2010 ||IPTG ||IPTG sensitive lysis cassette ||
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|-
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| <partinfo>BBa_K299806</partinfo> ||Warsaw ||2010 || ||MinC cell division inhibitor ||
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|-
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| <partinfo>BBa_K150007</partinfo> || Heidelberg||2008 ||Native Operon ||Colicin E1 ||
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|-
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| <partinfo>BBa_K150010</partinfo> || Heidelberg||2008 ||Native Operon ||Colicin E9 ||
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|-
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| <partinfo>BBa_K145230</partinfo> || KULeuven|| 2008|| HSL-LuxR|| ccdb ||
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| <partinfo>BBa_K117009</partinfo> ||NTU-Singapore ||2008 ||IPTG|| Colicin E7 ||
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|-
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| <partinfo>BBa_K117000</partinfo> ||NTU-Singapore ||2008 || || Lysin (activates Colicin E7) ||
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|-
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| <partinfo>BBa_K108001</partinfo> ||Tsinghua ||2008 || ||Enterobacter phage lysis ||
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|}
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[[File:Kill_Switch_Assay_1UBCiGEM2012.png||]]

Latest revision as of 17:10, 15 June 2012

British Columbia - 2012.igem.org [http://ung.igem.org/Previous_iGEM_Competitions Previous iGEM Competitions]

Team Abstracts: 2011 2010 2009 2008

Parts Registry: [http://partsregistry.org/Cell_death Cell Death]

Killswitch BioBricks
Numbers Team Year Trigger What it Does Where?
<partinfo>BBa_I716462</partinfo> UC-Berkeley 2007 Arabinose promoter BamHI
<partinfo>BBa_K512002</partinfo> USC2011 IPTG, targets GFP gene CRISPR-mediated GFP plasmid degradation
<partinfo>BBa_K112808</partinfo> UC-Berkeley 2008 No PromoterT4 Lysis device Plate 3, 6G
<partinfo>BBa_K541545</partinfo> FAGEM 2011 IPTG Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> METU-Ankara2011T7 Promoter ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> St. Andrews 2011 pBad Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.
Tokyo Metropolitan 2011 Killer Bee e.coli?
UNIST Korea 2011 Killed by high and low temps, not if dark.
Harvard 2010 Barnase cleaves RNA!
<partinfo>BBa_I716211</partinfo> UC Berkeley 2007 No Promoter Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB
Hong Kong 2010 Possible new killswitch, not implemented
<partinfo>BBa_K565001</partinfo> Valencia2011 ? 12 kb Colicin G
<partinfo>BBa_K565002</partinfo> Valencia 2011 ? 12 kb Colicin H
<partinfo>BBa_K565004</partinfo> Valencia 2011 No Promoter Microcin C51 production device (5 restriction sites)
<partinfo>BBa_K185004</partinfo> SJTU-BioX-Shanghai 2009 No Promoter RelE toxin+Double terminator
UNICAMP Brazil 2009 All sorts of kill switches, but may not all work in E. coli.
<partinfo>BBa_K284017</partinfo> UNICAMP-Brazil 2009 Lactate (Yeast) Lysozyme
<partinfo>BBa_K131000</partinfo> Calgary2008 SOS Promoter Colicin 2E3, 22A
<partinfo>BBa_K112806</partinfo> UC Berkeley 2008 T7 T4 Endolysin
<partinfo>BBa_K302035</partinfo> Newcastle 2010 Sucrose limit MazEF
<partinfo>BBa_K124014</partinfo> Brown 2008 No Promoter Mutated Holin Gene (Inconsistent sequencing)
<partinfo>BBa_K419003</partinfo> TzuChiU_Formosa 2010 pLysLysis Cassette - no antiholin
<partinfo>BBa_K317039</partinfo> Tokyo-NoKoGen 2010AHL AHL sensitive lysis cassette
<partinfo>BBa_K317040</partinfo> Tokyo-NoKoGen 2010 IPTG IPTG sensitive lysis cassette
<partinfo>BBa_K299806</partinfo> Warsaw 2010 MinC cell division inhibitor
<partinfo>BBa_K150007</partinfo> Heidelberg2008 Native Operon Colicin E1
<partinfo>BBa_K150010</partinfo> Heidelberg2008 Native Operon Colicin E9
<partinfo>BBa_K145230</partinfo> KULeuven 2008 HSL-LuxR ccdb
<partinfo>BBa_K117009</partinfo> NTU-Singapore 2008 IPTG Colicin E7
<partinfo>BBa_K117000</partinfo> NTU-Singapore 2008 Lysin (activates Colicin E7)
<partinfo>BBa_K108001</partinfo> Tsinghua 2008 Enterobacter phage lysis

Kill Switch Assay 1UBCiGEM2012.png

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