Team:WashU/Protocols/SDSPAGE15
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- | {{ | + | {{WashUbackprotocols}} |
<div id = "protocol"> | <div id = "protocol"> | ||
=SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel= | =SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel= | ||
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|4x Lower Tris | |4x Lower Tris | ||
|1.21875 ml | |1.21875 ml | ||
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|- | |- | ||
|20% SDS | |20% SDS | ||
|.250 ml | |.250 ml | ||
+ | |- | ||
+ | |10% Temed | ||
+ | |.0025 ml | ||
|- | |- | ||
|25% ammonium persulfate (APS) | |25% ammonium persulfate (APS) | ||
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|4x Upper Tris | |4x Upper Tris | ||
|.75 ml | |.75 ml | ||
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|- | |- | ||
|20% SDS | |20% SDS | ||
|.015 ml | |.015 ml | ||
+ | |- | ||
+ | |10% Temed | ||
+ | |.003 ml | ||
|- | |- | ||
|25% ammonium persulfate (APS) | |25% ammonium persulfate (APS) |
Latest revision as of 21:34, 9 August 2012