Latest revision as of 21:34, 9 August 2012

SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel

To create a 15% resolving gel with a 4% stacking gel, use:

**Table 1:** Preparation of the separating gel solution for 1 gel
	15%
dH₂O	1.2 ml
30% acrylamid/0.8% methylene bisacrylamide	2.5 mls
4x Lower Tris	1.21875 ml
20% SDS	.250 ml
10% Temed	.0025 ml
25% ammonium persulfate (APS)	.01 ml

To make the 4% stacking gel, use:

**Table 1:** Preparation of the stacking gel solution for 1 gel
	4%
dH₂O	1.845 ml
30% acrylamid/0.8% methylene bisacrylamide	.402 mls
4x Upper Tris	.75 ml
20% SDS	.015 ml
10% Temed	.003 ml
25% ammonium persulfate (APS)	.006 ml

@@ Line 1: / Line 1: @@
-{{WashUback}}
+{{WashUbackprotocols}}
 <div id = "protocol">
 =SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel=
@@ Line 17: / Line 17: @@
 |4x Lower Tris
 |1.21875 ml
-|-
-|10% Temed
-|.0025 ml
 |-
 |20% SDS
 |.250 ml
 |-
-|10% ammonium persulfate (APS)
+|10% Temed
+|.0025 ml
+|-
+|25% ammonium persulfate (APS)
 |.01 ml
 |}
@@ Line 43: / Line 43: @@
 |4x Upper Tris
 |.75 ml
-|-
-|10% Temed
-|.003 ml
 |-
 |20% SDS
 |.015 ml
 |-
-|10% ammonium persulfate (APS)
+|10% Temed
+|.003 ml
+|-
+|25% ammonium persulfate (APS)
 |.006 ml
 |}