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Revision as of 13:01, 1 August 2012 (view source) Sl.ilya (Talk | contribs) (Created page with "{{:Team:Technion/Template:Notebook|content= __NOTOC__ ==Ilya== - Cleaned the R0062+Fus1 #7 and R0062+Fus1 #8 after CIP. Concentrations: #7 - 27ng/ul, #8 - 35 ng/ul.<br> - Ligatio...") ← Older edit		Latest revision as of 13:03, 20 August 2012 (view source) Rachellev (Talk | contribs)
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	==Hila==		==Hila==
		+	Mini-prep and glycerol stock to the pTet + CFP / GFP bricks.<br>
	==Lior==		==Lior==
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	==Rachel==		==Rachel==
-		+	Yesterday I set up starters of pPROLAR (cerulean) on LB+KAN. Today I minipreped the plasmid. The concentrations of the plasmids are: F1 - 96 ng/ul; F2 -  137 ng/ul.
-	}}	+

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Latest revision as of 13:03, 20 August 2012

{{:Team:Technion/Template:Notebook|content=

Ilya

- Cleaned the R0062+Fus1 #7 and R0062+Fus1 #8 after CIP. Concentrations: #7 - 27ng/ul, #8 - 35 ng/ul.
- Ligation of R0062+Fus1 #7 and I0462. Ligation of R0062+Fus1 #8 and I0462. Also tried the #8 ligation with the sigma ligase, in order to see if it works in the NEB buffer.
- Transformation of ligation products.

Inbal

Asaf

Hila

Mini-prep and glycerol stock to the pTet + CFP / GFP bricks.

Lior

Noa

Evgeni

Shahar

Rachel

Yesterday I set up starters of pPROLAR (cerulean) on LB+KAN. Today I minipreped the plasmid. The concentrations of the plasmids are: F1 - 96 ng/ul; F2 - 137 ng/ul.