Team:WashU/Protocols/SDSPAGE15

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=SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel=
To create a 15% resolving gel with a 4% stacking gel, use:
To create a 15% resolving gel with a 4% stacking gel, use:
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|4x Lower Tris  
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|1.21875 ml
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|20% SDS
|20% SDS
|.250 ml
|.250 ml
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|10% ammonium persulfate (APS)
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|10% Temed
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|.0025 ml
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|25% ammonium persulfate (APS)
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|20% SDS
|20% SDS
|.015 ml
|.015 ml
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|10% ammonium persulfate (APS)
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|10% Temed
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|.003 ml
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|25% ammonium persulfate (APS)
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[https://2012.igem.org/Team:WashU/Protocols Back to Protocols]

Latest revision as of 21:34, 9 August 2012


SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel

To create a 15% resolving gel with a 4% stacking gel, use:

Table 1: Preparation of the separating gel solution for 1 gel
15%
dH2O 1.2 ml
30% acrylamid/0.8% methylene bisacrylamide 2.5 mls
4x Lower Tris 1.21875 ml
20% SDS .250 ml
10% Temed .0025 ml
25% ammonium persulfate (APS) .01 ml

To make the 4% stacking gel, use:

Table 1: Preparation of the stacking gel solution for 1 gel
4%
dH2O 1.845 ml
30% acrylamid/0.8% methylene bisacrylamide .402 mls
4x Upper Tris .75 ml
20% SDS .015 ml
10% Temed .003 ml
25% ammonium persulfate (APS) .006 ml


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