Team:NCTU Formosa/Project

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!align="center"|[[Team:NCTU_Formosa|Home]]
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{{Team:NCTU_Formosa/header-project}}
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!align="center"|[[Team:NCTU_Formosa/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=NCTU_Formosa Official Team Profile]
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!align="center"|[[Team:NCTU_Formosa/Project|Project]]
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!align="center"|[[Team:NCTU_Formosa/Parts|Parts Submitted to the Registry]]
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<p id="back-top"><a href="#top"><span></span>Back to Top</a></p>
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!align="center"|[[Team:NCTU_Formosa/Modeling|Modeling]]
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!align="center"|[[Team:NCTU_Formosa/Notebook|Notebook]]
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!align="center"|[[Team:NCTU_Formosa/Safety|Safety]]
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!align="center"|[[Team:NCTU_Formosa/Attributions|Attributions]]
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project">Introduction</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project"><p>Introduction</p></a>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-1">Enzyme for Isobutanol</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-1"><p>Enzyme for Isobutanol</p></a>
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== '''Overall project''' ==
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-2">Temperature Control System</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-2"><p>Temperature Control System</p></a>
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Our team invented an enzyme pathway for E. coli to produce butanol, which is closely related chemically to ethanol. However, the two extra carbon atoms and their attendant hydrogens let it be a higher energy value. In terms of storage and handling, it is less corrosive than ethanol and has several other advantages. Therefore, it may be a better substitution to ethanol as a biofuel.<br><br>
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The pathway starts at glucose, through glycolysis it will convert to pyruvate. Then the pyruvate goes through enzymatic process and produces isobutyraldehyde, which is toxic to the E.coli. To prevent the toxicity harming E.coli, we use the temperature regulation system.
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The first part is [[The 37℃ part]]. Our team makes E. coli produce a lot of nontoxic 2-Ketoisovalerate. Afterwards we reduce the temperature to 30℃.[[The 30℃ part ]] converts 2-ketoisovalerate to isobutyraldehyde at one blast. Then through simple process we get isobutanol, the potential biofuel in the future.<br><br>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-3">Zinc Finger</a></h1>
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What worth mentioning is that our team adds various ''zinc fingers'' before each enzyme to increase the affinity and efficiency of gene expression. Also, we are planning to design a container to produce and concentrate isobutanol. [[Team:NCTU_Formosa|NCTU_Formosa]] hopes to soon provide an affordable and “green” transportation fuel to the world!<br><br>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-3"><p>Zinc Finger</p></a>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-4">Instrument</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub2#sub2-4"><p>Instrument</p></a>
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== '''Project Details''' ==
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub3">Conclusion</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub3"><p>Conclusion</p></a>
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub4">Optimization</a></h1>
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub4"><p>Optimization</p></a>
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=== The 37℃ part===
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      <h1><a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub5">Future Works</a></h1>
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=== The 30℃ part===
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      <a href="https://2012.igem.org/Team:NCTU_Formosa/Project-sub5"><p>Future Works</p></a>
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==='''Result'''===
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== '''ZINC FINGER''' ==
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<b>A zinc finger is a kind of finger-shaped protein that containsone or more zincions to stabilize the structure. Proteins that contain zinc fingers(zinc finger protein) are the most common transcription factors in organisms. A transcription factor is a protein that binds to DNA and controls the expression of genes. The zinc finger proteins bindspecific DNA, RNA, or other molecules with the interaction of its domain and these molecules.<br><br>
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<h1 id="project-s1-title" class="project-s-title"><a name="sub1"> </a> <span>Introduction to the project</span></h1>
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<p>Nowadays, environmental pollution and energy depletion have become crucial problems. We need to find alternative energy to replace the running out fossil fuel. Due to the pollution issues, this alternative energy should be environmental friendly. Up until now, ethanol is the most common biomass fuel because the final product is harmless water. However, ethanol will corrode metallic  surface of the engines lead to higher cost than fossil fuel usage. Unlike ethanol, isobutanol do not corrode metal and contain higher ratio of the heat of combustion than ethanol. Besides, as well as ethanol, isobutanol doesn’t produce pollutants such as sulfur dioxide, nitric oxide and nitric dioxide. Isobutanol has widely utilized in many applications as a organic solvent, and antifreeze. As what we want, to get clean energy, we chose isobutanol to be our project. We believe that isobutanol is a potential eco fuel in the future. However, currently isobutanol production wasn't very promising. According to the previous studies, the low yield of isobutanol was caused by the toxicity of isobutanol which would kill the host <i>E.coli</i> . In this study, we introduced two innovative and brilliant solutions to solve this serious problem. Now, let’s take a deeper look in our new ideas!</p></div>
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We add zif268,PBSII,HIVC and Gli1 four zinc fingers separately in front of our biopart: Alss, ilvc, ilvD, kivd. We expect that the enzyme with zinc fingers would increase the productivity of isobutanol.<br><br>
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=== zif268 ===
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This  zinc finger identifies the DNA sequence 5'-GCG TGG GCG-3',binding to the certain domain of the sequence.<br><br><br><br><br><br><br><br><br><br>
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[[image:Zif268.jpg|thumb|Zif268 Binding With DNA]]
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<div id="counter"><img src="https://static.igem.org/mediawiki/2012/d/d1/NctuCapture.jpg" /><img src="http://s11.flagcounter.com/count/Rhz/bg_FFFFFF/txt_000000/border_CCCCCC/columns_2/maxflags_248/viewers_3/labels_1/pageviews_1/flags_1/" /><img src="https://static.igem.org/mediawiki/2012/d/d1/NctuCapture.jpg" /></div>
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    <p>Copyright © 2012 NCTU Formosa</p>
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=== PBSII  ===
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<p>Background image belongs to <a href="http://fatezoom.deviantart.com/" target="_blank">Yuri Kormin</a>.</p>
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Identifies DNA sequence 5'-GTG TGG AAA-3'.<br><br>
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    <p class="author">Website designed by Calvin Hue.</p>
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=== HivC  ===
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    </div> </div>
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Identifies DNA sequence 5'-GAT GCT GCA-3'.<br><br>
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=== Gli1  ===
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Identifies DNA sequence 5'-GAC CAC CCA-3'.<br><br>
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[[image:2GLI.pdb1.jpg|thumb|Five-Finger GLI/DNA Complex]]
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Latest revision as of 15:15, 26 October 2012

Team:NCTU Formosa - 2012.igem.org

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 Introduction to the project

Nowadays, environmental pollution and energy depletion have become crucial problems. We need to find alternative energy to replace the running out fossil fuel. Due to the pollution issues, this alternative energy should be environmental friendly. Up until now, ethanol is the most common biomass fuel because the final product is harmless water. However, ethanol will corrode metallic surface of the engines lead to higher cost than fossil fuel usage. Unlike ethanol, isobutanol do not corrode metal and contain higher ratio of the heat of combustion than ethanol. Besides, as well as ethanol, isobutanol doesn’t produce pollutants such as sulfur dioxide, nitric oxide and nitric dioxide. Isobutanol has widely utilized in many applications as a organic solvent, and antifreeze. As what we want, to get clean energy, we chose isobutanol to be our project. We believe that isobutanol is a potential eco fuel in the future. However, currently isobutanol production wasn't very promising. According to the previous studies, the low yield of isobutanol was caused by the toxicity of isobutanol which would kill the host E.coli . In this study, we introduced two innovative and brilliant solutions to solve this serious problem. Now, let’s take a deeper look in our new ideas!