Team:TU-Eindhoven

From 2012.igem.org

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Latest revision as of 23:32, 26 September 2012

Welcome to the home page of the iGEM team of the Eindhoven University of Technology!

Eindhoven, the city famous for its light bulbs, is the place where the roots of Dutch television lie. We, the iGEM team of the Eindhoven University of Technology, developed an innovative piece of electro-biological equipment which will replace your old television screen in the future! We proudly present to you...

It's a multi-colored display, in which genetically engineered yeast cells are electrically stimulated to induce a fluorescent light response and consequently function as pixels. Since calcium takes the leading part in this process, the yeast cells are engineered with fluorescent calcium sensors and extra voltage-gated calcium channels. Please take a look around at our site! Besides all important issues with regard to our project, you can find more information about us, our outreach, our partners and of course some nice pictures! Enjoy!

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+<h3>Welcome to the home page of the iGEM team of the Eindhoven University of Technology!</h3>
+<p>
+Eindhoven, the city famous for its light bulbs, is the place where the roots of Dutch television lie. We, the iGEM team of the Eindhoven University of Technology, developed an innovative piece of <span class="yellow">electro-biological  equipment</span> which will replace your old television screen in the future! We proudly present to you...
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+<html><iframe width="730" height="413" src="http://www.youtube.com/embed/22A2bDACSMc" frameborder="0" allowfullscreen style="margin-left: -10px;"></iframe></html>
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+It's a <span class="yellow">multi-colored display</span>, in which genetically engineered <span class="yellow">yeast cells</span> are electrically stimulated to induce a fluorescent light response and consequently function as pixels. Since calcium takes the leading part in this process, the yeast cells are engineered with <span class="yellow">fluorescent calcium sensors</span> and <span class="yellow">extra voltage-gated calcium channels</span>.
+Please take a look around at our site! Besides all important issues with regard to our project, you can find more information about us, our outreach, our partners and of course some nice pictures! Enjoy!
+</p>
+<!-- {{:Team:TU-Eindhoven/Templates/title|colour=#386abc|title=The Project}} -->
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-This year's project will be all about [https://2012.igem.org/Team:TU-Eindhoven/LEC '''Light Emitting Cells'''] (LECs) that emit light in response to an electric stimulus. A LEC has to emit light conditionally, that is, it has to react a control signal. In our case the control signal will be an electric field that is strong enough to depolarize the plasma membrane of ''Saccharomyces cerevisiae'' cells, but weak enough for the cells to survive.
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-The plasma membrane of ''S. cerevisiae'' contains the CCH1-MID1 channel protein complex that is homologous to mammalian Voltage Gated Calcium Channels (VGCCs). It is hypothesized that upon depolarization of the plasma membrane, calcium ions selectively enter the cytoplasm through CCH1-MID1. Light will be emitted through the fluorescence of GECO protein, a calcium sensor that is expressed from an genetically engineered plasmid. When the calcium concentration is high the GECO proteins will be fluorescent, when the calcium concentration is low the GECO proteins will not be fluorescent. After a while the calcium concentration will drop to homeostatic levels through active transport to the yeast's vacuole and fluorescence will cease.
-In conclusion: An electric field is used to control the fluorescence of a calcium sensor protein in a yeast cell.
-{{:Team:TU-Eindhoven/Templates/h2|header=Device}}
-Introduction to the [https://2012.igem.org/Team:TU-Eindhoven/LEC/Device '''device'''] for stimulation of LECs.
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