Team:TU-Eindhoven

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<h3>Welcome to the home page of the iGEM team of the Eindhoven University of Technology!</h3>
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Eindhoven, the city famous for its light bulbs, is the place where the roots of Dutch television lie. We, the iGEM team of the Eindhoven University of Technology, developed an innovative piece of <span class="yellow">electro-biological  equipment</span> which will replace your old television screen in the future! We proudly present to you...
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<div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div>
 
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<div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div>
 
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Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''biosensors'''] and as [https://2010.igem.org/Team:Cambridge/Tools/Lighting '''natural light sources''']. We also developed software tools to aid construction of BioBrick parts and devices.
 
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We adopted a number of strategies to extend the use of '''firefly luciferase''':
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* [https://2010.igem.org/Team:Cambridge/Codons '''Codon optimisation'''] for increased light output
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* Use of a [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration '''luciferin regenerating enzyme'''].
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* Mutagenesis to create a number of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour '''different colours''']
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It's a <span class="yellow">multi-colored display</span>, in which genetically engineered <span class="yellow">yeast cells</span> are electrically stimulated to induce a fluorescent light response and consequently function as pixels. Since calcium takes the leading part in this process, the yeast cells are engineered with <span class="yellow">fluorescent calcium sensors</span> and <span class="yellow">extra voltage-gated calcium channels</span>.
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Please take a look around at our site! Besides all important issues with regard to our project, you can find more information about us, our outreach, our partners and of course some nice pictures! Enjoy!
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  <div style="position:absolute; width:230px; left:5px; text-align:center;">Description of box 1.</div>
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''.  We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBrick'''] to emit light in normal E. coli strains without the addition of any external substrate.
 
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During our project we made extensive use of [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''] to manufacture our parts, and have submitted an [https://2010.igem.org/Team:Cambridge/Gibson/RFC '''RFC'''] to the [http://bbf.openwetware.org/ '''BioBricks Foundation'''] to help future teams make best use of this technique.
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Please see [https://igem.org/Results '''iGEM official results page'''] to see how all the teams did.
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Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
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* [https://2010.igem.org/Team:Cambridge/Tools/Gibson '''Gibthon Construct Designer'''] allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''].
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* [https://2010.igem.org/Team:Cambridge/Tools/GenBank '''BioBrick → GenBank'''] allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Ligate '''Ligation Calculator'''] is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence.  It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''quantitative biosensors'''].
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[[Image:EindhovenTeam.jpg|center|frame|The team - in order - Member A, Member B, Member C.]]
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* Finalist
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* Winner of "Best Wiki" award (awarded jointly to Cambridge and [https://2010.igem.org/Team:Imperial_College_London '''Imperial College London'''])
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* Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
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* Awarded a Gold Medal
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Please see [http://https://igem.org/Results '''iGEM official results page'''] to see how all the teams did.
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* [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article]
 
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* [http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html Daily Mail article]
 
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* [http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html The Telegraph Article]
 
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[[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins.]]
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Latest revision as of 23:32, 26 September 2012

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