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Team:Penn State/Parts
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<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span> | <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span> | ||
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span> | <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span> | ||
| - | <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling"> | + | <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span> |
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span> | <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span> | ||
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a> | <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a> | ||
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<th scope="col">Sequence Name</th> | <th scope="col">Sequence Name</th> | ||
<th scope="col">Status</th> | <th scope="col">Status</th> | ||
| - | + | ||
<tr> | <tr> | ||
<td>theonine 9-repeat codon_1</td> | <td>theonine 9-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | |||
</tr> | </tr> | ||
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<td>theonine 9-repeat codon_2</td> | <td>theonine 9-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>theonine 9-repeat codon_3</td> | <td>theonine 9-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>theonine 9-repeat codon_alternating</td> | <td>theonine 9-repeat codon_alternating</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>alanine 9-repeat codon_1</td> | <td>alanine 9-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>alanine 9-repeat codon_2</td> | <td>alanine 9-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | |||
</tr> | </tr> | ||
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<td>alanine 9-repeat codon_3</td> | <td>alanine 9-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | |||
</tr> | </tr> | ||
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<td>alanine 9-repeat codon_alternating</td> | <td>alanine 9-repeat codon_alternating</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>theonine 6-repeat codon_1</td> | <td>theonine 6-repeat codon_1</td> | ||
| - | <td>complete</td> | + | <td>complete and sequenced</td> |
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>theonine 6-repeat codon_2</td> | <td>theonine 6-repeat codon_2</td> | ||
| - | <td>complete</td> | + | <td>complete and sequenced</td> |
<td>yes</td> | <td>yes</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>theonine 6-repeat codon_3</td> | <td>theonine 6-repeat codon_3</td> | ||
<td>complete</td> | <td>complete</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>theonine 6-repeat codon_alternating</td> | <td>theonine 6-repeat codon_alternating</td> | ||
<td>complete</td> | <td>complete</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>alanine 6-repeat codon_1</td> | <td>alanine 6-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>alanine 6-repeat codon_2</td> | <td>alanine 6-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>alanine 6-repeat codon_3</td> | <td>alanine 6-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>alanine 6-repeat codon_alternating</td> | <td>alanine 6-repeat codon_alternating</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>threonine 3-repeat codon_1</td> | <td>threonine 3-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>threonine 3-repeat codon_2</td> | <td>threonine 3-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<td>threonine 3-repeat codon_3</td> | <td>threonine 3-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
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<th scope="col">Sequence Name</th> | <th scope="col">Sequence Name</th> | ||
<th scope="col">Status</th> | <th scope="col">Status</th> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2weak small length</td> | <td>RBS1strong_RBS2weak small length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1weak_RBS2strong small length</td> | <td>RBS1weak_RBS2strong small length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2strong small length</td> | <td>RBS1strong_RBS2strong small length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2weak med length</td> | <td>RBS1strong_RBS2weak med length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1weak_RBS2strong med length</td> | <td>RBS1weak_RBS2strong med length</td> | ||
| - | <td>complete</td> | + | <td>complete and sequenced</td> |
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2strong med length</td> | <td>RBS1strong_RBS2strong med length</td> | ||
<td>order oligos</td> | <td>order oligos</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2weak med length</td> | <td>RBS1strong_RBS2weak med length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1weak_RBS2strong med length</td> | <td>RBS1weak_RBS2strong med length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2strong med length</td> | <td>RBS1strong_RBS2strong med length</td> | ||
<td>order oligos</td> | <td>order oligos</td> | ||
| - | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 03:01, 27 October 2012
Parts Submitted to the Registry
Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!
Submitted Parts
| Name | Type | Description | Symbolic Model | Length (bp) |
|---|---|---|---|---|
| BBa_K933000 | device | multiple start codons coding for RFP and sfGFP respectively | design | |
| BBa_K933001 | device | repeating threonine 6-codon sequence with mCherry and GFP reporters | design | |
| BBa_K933002 | device | AHL leader with mCherry and GFP reporters | design |
Continuing Research
Codon Optimization
| Sequence Name | Status | |
|---|---|---|
| theonine 9-repeat codon_1 | oligos annealed | |
| theonine 9-repeat codon_2 | oligos annealed | |
| theonine 9-repeat codon_3 | oligos annealed | |
| theonine 9-repeat codon_alternating | oligos annealed | |
| alanine 9-repeat codon_1 | oligos annealed | |
| alanine 9-repeat codon_2 | oligos annealed | |
| alanine 9-repeat codon_3 | oligos annealed | |
| alanine 9-repeat codon_alternating | oligos annealed | |
| theonine 6-repeat codon_1 | complete and sequenced | |
| theonine 6-repeat codon_2 | complete and sequenced | yes |
| theonine 6-repeat codon_3 | complete | |
| theonine 6-repeat codon_alternating | complete | |
| alanine 6-repeat codon_1 | oligos annealed | |
| alanine 6-repeat codon_2 | oligos annealed | |
| alanine 6-repeat codon_3 | oligos annealed | |
| alanine 6-repeat codon_alternating | oligos annealed | |
| threonine 3-repeat codon_1 | oligos annealed | |
| threonine 3-repeat codon_2 | oligos annealed | |
| threonine 3-repeat codon_3 | oligos annealed | |
| threonine 3-repeat codon_alternating | oligos annealed |
Bidirectional Promoters
| Sequence Name | Status | Part Sequenced |
|---|---|---|
| BBa_K933004 | complete | yes |
| BBa_K933005 | complete and added to Registry | yes |
| BBa_K933006 | complete and added to Registry | yes |
| BBa_K933003 | complete and added to Registry | yes |
| testing promoter BBa_J23103 | oligos annealed | |
| testing promoter BBa_J23114 | oligos annealed |
Multiple Start Codons
| Sequence Name | Status |
|---|---|
| RBS1strong_RBS2weak small length | oligos annealed |
| RBS1weak_RBS2strong small length | oligos annealed |
| RBS1strong_RBS2strong small length | oligos annealed |
| RBS1strong_RBS2weak med length | oligos annealed |
| RBS1weak_RBS2strong med length | complete and sequenced |
| RBS1strong_RBS2strong med length | order oligos |
| RBS1strong_RBS2weak med length | oligos annealed |
| RBS1weak_RBS2strong med length | oligos annealed |
| RBS1strong_RBS2strong med length | order oligos |
