Team:Penn State/Parts

From 2012.igem.org

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Parts Submitted to the Registry

Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!

Registry of Biological Parts

Submitted Parts

Name	Type	Description	Symbolic Model
BBa_K933000	device	multiple start codons coding for RFP and sfGFP respectively	design
BBa_K933001	device	repeating threonine 6-codon sequence with mCherry and GFP reporters	design
BBa_K933002	device	AHL leader with mCherry and GFP reporters	design

Continuing Research

Codon Optimization

Sequence Name	Status
theonine 9-repeat codon_1	oligos annealed
theonine 9-repeat codon_2	oligos annealed
theonine 9-repeat codon_3	oligos annealed
theonine 9-repeat codon_alternating	oligos annealed
alanine 9-repeat codon_1	oligos annealed
alanine 9-repeat codon_2	oligos annealed
alanine 9-repeat codon_3	oligos annealed
alanine 9-repeat codon_alternating	oligos annealed
theonine 6-repeat codon_1	complete and sequenced
theonine 6-repeat codon_2	complete and sequenced	yes
theonine 6-repeat codon_3	complete
theonine 6-repeat codon_alternating	complete
alanine 6-repeat codon_1	oligos annealed
alanine 6-repeat codon_2	oligos annealed
alanine 6-repeat codon_3	oligos annealed
alanine 6-repeat codon_alternating	oligos annealed
threonine 3-repeat codon_1	oligos annealed
threonine 3-repeat codon_2	oligos annealed
threonine 3-repeat codon_3	oligos annealed
threonine 3-repeat codon_alternating	oligos annealed

Bidirectional Promoters

Sequence Name	Status	Part Sequenced
BBa_K933004	complete	yes
BBa_K933005	complete and added to Registry	yes
BBa_K933006	complete and added to Registry	yes
BBa_K933003	complete and added to Registry	yes
testing promoter BBa_J23103	oligos annealed
testing promoter BBa_J23114	oligos annealed

Multiple Start Codons

Sequence Name	Status
RBS1strong_RBS2weak small length	oligos annealed
RBS1weak_RBS2strong small length	oligos annealed
RBS1strong_RBS2strong small length	oligos annealed
RBS1strong_RBS2weak med length	oligos annealed
RBS1weak_RBS2strong med length	complete and sequenced
RBS1strong_RBS2strong med length	order oligos
RBS1strong_RBS2weak med length	oligos annealed
RBS1weak_RBS2strong med length	oligos annealed
RBS1strong_RBS2strong med length	order oligos