Team:SUSTC-Shenzhen-B

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                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
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                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Achievements</a></li>
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                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li>
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<article>
<article>
                                 <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
                                 <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
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<p>We used both
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<p><b>Welcome!!</b></p>
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<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm"> theoretical model</a> and
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<p>We are sophomores at SUSTC. During this summer, we studied the efficiency of rho-independent
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<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experiment</a> method.
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transcription terminators using both
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to study the rho-independent transcription terminators which regulate gene expression.</p>
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theoretical modeling
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<p> First, we developed a theoretical model to calculate the free energy of RNA folding and predict the secondary structure of terminators. An <a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">algorithm </a> was proposed to predict terminator efficiency from the secondary structure.</p>
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and  
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<p>We also constructed 100 terminators and did some wetlab experiments to validate our theoretical prediction. The basic method was measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.</p>
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experimental methods.  
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<p>Besides,we developed a <a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">software</a> and a <a href="http://www.terminatorefficiency.com/"> web server</a> for people to calculate terminator efficiency. We also built a
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Here is the  
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<a href="http://www.terminatorefficiency.com/Database.html">database</a>
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<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">overview </a>
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  of terminator efficiency which we believe is the largest database of such kind. </p>
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of our project.
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<p>Hope our work is useful for the comprehensive study on terminator efficiency.</p>
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</p>
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                 </article>
                 </article>

Latest revision as of 03:19, 27 October 2012

Title

Transcriptional Terminator Efficiency Calculator (TTEC)

Welcome!!

We are sophomores at SUSTC. During this summer, we studied the efficiency of rho-independent transcription terminators using both theoretical modeling and experimental methods. Here is the overview of our project.

South University of Science and Technology of China

Retrieved from "https://2012.igem.org/Template:SUSTC-B1"

Retrieved from "http://2012.igem.org/Team:SUSTC-Shenzhen-B"