Team:SUSTC-Shenzhen-B

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<article>
<article>
                                 <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
                                 <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
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<p>We studied the rho-independent transcription terminators which regulate gene expression using both  
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<p><b>Welcome!!</b></p>
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<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm"> theoretical model</a>and
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<p>We are sophomores at SUSTC. During this summer, we studied the efficiency of rho-independent  
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<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experiment</a>method.</p>
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transcription terminators using both  
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<p> We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.</p>
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theoretical modeling
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<p> To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.</p>
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and  
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<p> We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.</p>
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experimental methods.
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Here is the
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<a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">overview </a>
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of our project.
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</p>
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Latest revision as of 03:19, 27 October 2012

Title

Transcriptional Terminator Efficiency Calculator (TTEC)

Welcome!!

We are sophomores at SUSTC. During this summer, we studied the efficiency of rho-independent transcription terminators using both theoretical modeling and experimental methods. Here is the overview of our project.

South University of Science and Technology of China

Retrieved from "https://2012.igem.org/Template:SUSTC-B1"

Retrieved from "http://2012.igem.org/Team:SUSTC-Shenzhen-B"