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Team:Cornell/notebook/strain list
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<h2 class="centered">Strain List</h2> | <h2 class="centered">Strain List</h2> | ||
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<div class="row last-ele"> | <div class="row last-ele"> | ||
| - | + | <table border="2"> | |
| - | + | <tr> | |
| - | <tr> | + | <th>CUGEM ID</th><th>Strain Species</th><th>Strain name</th><th>Brief Description</th><th>Plasmid conferred resistances</th><th>Strain Genotype</th><th>BioBrick Insert ID</th><th>Backbone Name</th><th>Plasmid details </th> |
| - | <th>CUGEM ID</th><th>Strain Species</th><th>Strain name</th><th>Brief Description</th><th>Plasmid conferred resistances</th><th>Strain Genotype</th><th>BioBrick Insert ID</th><th>Backbone Name</th><th>Plasmid details | + | </tr> |
| - | </th> | + | <tr> |
| - | </tr> | + | <td>S0000</td><td>E. coli</td><td>DH5a</td><td></td><td>N/A</td><td></td><td>N/A</td><td>N/A</td><td>N/A </td> |
| - | <tr> | + | </tr> |
| - | <td>S0000</td><td>E. coli</td><td>DH5a</td><td></td><td>N/A</td><td></td><td>N/A</td><td>N/A</td><td>N/A | + | <tr> |
| - | </td> | + | <td>S0001</td><td>S. oneidensis </td><td>JG700</td><td></td><td>N/A</td><td>_mtrB</td><td>N/A</td><td>N/A</td><td>N/A </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0001</td><td>S. oneidensis </td><td>JG700</td><td></td><td>N/A</td><td>_mtrB</td><td>N/A</td><td>N/A</td><td>N/A | + | <td>S0002</td><td>S. oneidensis </td><td>JG1194</td><td></td><td>N/A</td><td>_Mtr</td><td>N/A</td><td>N/A</td><td>N/A </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0003</td><td>S. oneidensis </td><td>JG1519</td><td></td><td>N/A</td><td>_Mtr/_mtrB</td><td>N/A</td><td>N/A</td><td>N/A </td> |
| - | <td>S0002</td><td>S. oneidensis </td><td>JG1194</td><td></td><td>N/A</td><td>_Mtr</td><td>N/A</td><td>N/A</td><td>N/A | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0004</td><td>S. oneidensis </td><td>JG1220</td><td></td><td>kan</td><td>JG700 (S1)</td><td>N/A</td><td>?</td><td>pmtrB </td> |
| - | <tr> | + | </tr> |
| - | <td>S0003</td><td>S. oneidensis </td><td>JG1519</td><td></td><td>N/A</td><td>_Mtr/_mtrB</td><td>N/A</td><td>N/A</td><td>N/A | + | <tr> |
| - | </td> | + | <td>S0005</td><td>S. oneidensis </td><td>JG1219</td><td></td><td>kan</td><td>JG700 (S1)</td><td>N/A</td><td>?</td><td>pmtrE </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0004</td><td>S. oneidensis </td><td>JG1220</td><td></td><td>kan</td><td>JG700 (S1)</td><td>N/A</td><td>?</td><td>pmtrB | + | <td>S0006</td><td>S. oneidensis </td><td>JG1527</td><td></td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>?</td><td>pmtrB/mtrC/mtrA </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0007</td><td>S. oneidensis </td><td>JG1531</td><td></td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>?</td><td>pmtrE/mtrC/mtrA </td> |
| - | <td>S0005</td><td>S. oneidensis </td><td>JG1219</td><td></td><td>kan</td><td>JG700 (S1)</td><td>N/A</td><td>?</td><td>pmtrE | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0008</td><td>S. oneidensis </td><td>JG1537</td><td>Backbone (control)</td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>pBBR-BB</td><td>Empty backbone </td> |
| - | <tr> | + | </tr> |
| - | <td>S0006</td><td>S. oneidensis </td><td>JG1527</td><td></td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>?</td><td>pmtrB/mtrC/mtrA | + | <tr> |
| - | </td> | + | <td>S0009</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 1 (Failed nah operon) </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0007</td><td>S. oneidensis </td><td>JG1531</td><td></td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>?</td><td>pmtrE/mtrC/mtrA | + | <td>S0010</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 2 (Failed nah operon) </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0011</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 3 (Failed nah operon) </td> |
| - | <td>S0008</td><td>S. oneidensis </td><td>JG1537</td><td>Backbone (control)</td><td>kan</td><td>JG1519 (S3)</td><td>N/A</td><td>pBBR-BB</td><td>Empty backbone | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0012</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>kan</td><td>DH5a</td><td>N/A</td><td></td><td>pBBRBB-eGFP </td> |
| - | <tr> | + | </tr> |
| - | <td>S0009</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 1 (Failed nah operon) | + | <tr> |
| - | </td> | + | <td>S0013</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>SP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0010</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 2 (Failed nah operon) | + | <td>S0014</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>SP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0015</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>Shweta's (either SP1 or SP2?) </td> |
| - | <td>S0011</td><td>E. coli</td><td>DH5a</td><td>Failure</td><td>amp</td><td>DH5a</td><td>N/A</td><td>pBMT-1</td><td>Gibson 3 (Failed nah operon) | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0016</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>TS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) </td> |
| - | <tr> | + | </tr> |
| - | <td>S0012</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>kan</td><td>DH5a</td><td>N/A</td><td></td><td>pBBRBB-eGFP | + | <tr> |
| - | </td> | + | <td>S0017</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>TS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0013</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>SP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) | + | <td>S0018</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>cm</td><td>DH5a</td><td>BBa_J04450</td><td>pSB3C5</td><td>mRFP1 in MCS; p15a origin; </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0019</td><td>E. coli</td><td>WM3064</td><td>Conjugation strain</td><td>N/A</td><td>DAP auxotroph</td><td>N/A</td><td></td><td></td> |
| - | <td>S0014</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>SP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0020a</td><td>S. oneidensis </td><td>CUGEM001</td><td>ARS Reporter</td><td>kan</td><td>JG700 (S1)</td><td>?</td><td></td><td>ARS Reporter w/ RBS swap (Shweta's) </td> |
| - | <tr> | + | </tr> |
| - | <td>S0015</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>Shweta's (either SP1 or SP2?) | + | <tr> |
| - | </td> | + | <td>S0021</td><td>P. putida</td><td>G7</td><td>Wildtype</td><td>N/A</td><td>NAH7</td><td>N/A</td><td></td><td></td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0016</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>TS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) | + | <td>S0022</td><td>E. coli</td><td>DH5a</td><td>Salicylate sensing</td><td>amp</td><td>DH5a</td><td>BBa_K228227</td><td>pSB4A5</td><td>"Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results." </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0023</td><td>E. coli</td><td>DH5a</td><td>Nitrogen sensing</td><td>amp</td><td>DH5a</td><td>BBa_K216005</td><td>pSB1A2</td><td>"PyeaR promoter, responsive to nitrate, nitrite and nitric oxide" </td> |
| - | <td>S0017</td><td>E. coli</td><td>DH5a</td><td>ARS Reporter</td><td>kan</td><td>DH5a</td><td>?</td><td>pBBR-BB</td><td>TS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0024</td><td>E. coli</td><td>DH5a</td><td>oriT</td><td>amp</td><td>DH5a</td><td>BBa_J01003</td><td>pSB1A2</td><td>"Origin of transfer, homologous to mob gene in PBRBB" </td> |
| - | <tr> | + | </tr> |
| - | <td>S0018</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>cm</td><td>DH5a</td><td>BBa_J04450</td><td>pSB3C5</td><td>mRFP1 in MCS; p15a origin; | + | <tr> |
| - | </td> | + | <td>S0025</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23100</td><td>BBa_J61002</td><td>Constitutive promoter (1); mRFP downsteam; pMB1 origin; </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0019</td><td>E. coli</td><td>WM3064</td><td>Conjugation strain</td><td>N/A</td><td>DAP auxotroph</td><td>N/A</td><td></td><td> | + | <td>S0026</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23101</td><td>BBa_J61002</td><td>Constitutive promoter (0.7); mRFP downsteam; pMB1 origin; </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0027</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23106</td><td>BBa_J61002</td><td>Constitutive promoter (0.4); mRFP downsteam; pMB1 origin; </td> |
| - | <td>S0020a</td><td>S. oneidensis </td><td>CUGEM001</td><td>ARS Reporter</td><td>kan</td><td>JG700 (S1)</td><td>?</td><td></td><td>ARS Reporter w/ RBS swap (Shweta's) | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0028</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23105</td><td>BBa_J61002</td><td>Constitutive promoter (0.24); mRFP downsteam; pMB1 origin; </td> |
| - | <tr> | + | </tr> |
| - | <td>S0021</td><td>P. putida</td><td>G7</td><td>Wildtype</td><td>N/A</td><td>NAH7</td><td>N/A</td><td></td><td> | + | <tr> |
| - | </td> | + | <td>S0029</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23114</td><td>BBa_J61002</td><td>Constitutive promoter (0.1); mRFP downsteam; pMB1 origin; </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0022</td><td>E. coli</td><td>DH5a</td><td>Salicylate sensing</td><td>amp</td><td>DH5a</td><td>BBa_K228227</td><td>pSB4A5</td><td>"Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results." | + | <td>S0030</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23103</td><td>BBa_J61002</td><td>Constitutive promoter (0.01); mRFP downsteam; pMB1 origin; </td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0031</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>cm</td><td>DH5a</td><td>BBa_J04450</td><td>pSB1C3</td><td>mRFP1 in MCS; pMB1 origin; </td> |
| - | <td>S0023</td><td>E. coli</td><td>DH5a</td><td>Nitrogen sensing</td><td>amp</td><td>DH5a</td><td>BBa_K216005</td><td>pSB1A2</td><td>"PyeaR promoter, responsive to nitrate, nitrite and nitric oxide" | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0032</td><td>E. coli</td><td>DH5a</td><td>nah Operon</td><td>cm</td><td>DH5a</td><td></td><td>pSB1C3</td><td>nah operon in MCS; pBM1 origin; </td> |
| - | <tr> | + | </tr> |
| - | <td>S0024</td><td>E. coli</td><td>DH5a</td><td>oriT</td><td>amp</td><td>DH5a</td><td>BBa_J01003</td><td>pSB1A2</td><td>"Origin of transfer, homologous to mob gene in PBRBB" | + | <tr> |
| - | </td> | + | <td>S0033</td><td>E. coli</td><td>DH5a</td><td>lac inducible mtrB</td><td>kan</td><td>DH5a</td><td>BBa_K098984</td><td>pSB3K3</td><td>lac indubible mtrB in MCS; p15a origin; </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0025</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23100</td><td>BBa_J61002</td><td>Constitutive promoter (1); mRFP downsteam; pMB1 origin; | + | <td>S0034</td><td>E. coli</td><td>DH5a</td><td>SAL (Salicylate Reporter)</td><td>kan</td><td>DH5a</td><td></td><td>pBBR-BB</td><td></td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0035</td><td>E. coli</td><td>DH5a</td><td>oriT + pSB3C5</td><td>cm</td><td>DH5a</td><td></td><td>pSB3C5</td><td></td> |
| - | <td>S0026</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23101</td><td>BBa_J61002</td><td>Constitutive promoter (0.7); mRFP downsteam; pMB1 origin; | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0036</td><td>E. coli</td><td>DH5a</td><td>nah Operon + p29</td><td>amp</td><td>DH5a</td><td></td><td>BBa_J61002</td><td></td> |
| - | <tr> | + | </tr> |
| - | <td>S0027</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23106</td><td>BBa_J61002</td><td>Constitutive promoter (0.4); mRFP downsteam; pMB1 origin; | + | <tr> |
| - | </td> | + | <td>S0037</td><td>E. coli</td><td>WM3064</td><td>p14 RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0028</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23105</td><td>BBa_J61002</td><td>Constitutive promoter (0.24); mRFP downsteam; pMB1 origin; | + | <td>S0038</td><td>E. coli</td><td>WM3064</td><td>p16 RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0039</td><td>E. coli</td><td>WM3064</td><td>p25BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | <td>S0029</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23114</td><td>BBa_J61002</td><td>Constitutive promoter (0.1); mRFP downsteam; pMB1 origin; | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0040</td><td>E. coli</td><td>WM3064</td><td>p27BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | <tr> | + | </tr> |
| - | <td>S0030</td><td>E. coli</td><td>DH5a</td><td>Promoter</td><td>amp</td><td>DH5a</td><td>BBa_J23103</td><td>BBa_J61002</td><td>Constitutive promoter (0.01); mRFP downsteam; pMB1 origin; | + | <tr> |
| - | </td> | + | <td>S0041</td><td>E. coli</td><td>WM3064</td><td>p29BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0031</td><td>E. coli</td><td>DH5a</td><td>Backbone</td><td>cm</td><td>DH5a</td><td>BBa_J04450</td><td>pSB1C3</td><td>mRFP1 in MCS; pMB1 origin; | + | <td>S0042</td><td>E. coli</td><td>WM3064</td><td>WM_p14</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0043</td><td>E. coli</td><td>WM3064</td><td>WM_p16</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | <td>S0032</td><td>E. coli</td><td>DH5a</td><td>nah Operon</td><td>cm</td><td>DH5a</td><td></td><td>pSB1C3</td><td>nah operon in MCS; pBM1 origin; | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0044</td><td>E. coli</td><td>WM3064</td><td>WM_SAL1</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | <tr> | + | </tr> |
| - | <td>S0033</td><td>E. coli</td><td>DH5a</td><td>lac inducible mtrB</td><td>kan</td><td>DH5a</td><td>BBa_K098984</td><td>pSB3K3</td><td>lac indubible mtrB in MCS; p15a origin; | + | <tr> |
| - | </td> | + | <td>S0045</td><td>E. coli</td><td>WM3064</td><td>p29_nah_oriT_p17c</td><td>cm</td><td>WM3064</td><td></td><td>pSB3C5</td><td>Constitutively expressed nah operon w/ oriT </td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td>S0034</td><td>E. coli</td><td>DH5a</td><td>SAL (Salicylate Reporter)</td><td>kan</td><td>DH5a</td><td></td><td>pBBR-BB</td><td> | + | <td>S0046</td><td>E. coli</td><td>WM3064</td><td>SAL_RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | </td> | + | </tr> |
| - | </tr> | + | <tr> |
| - | <tr> | + | <td>S0047</td><td>E. coli</td><td>WM3064</td><td>SAL2</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td></td> |
| - | <td>S0035</td><td>E. coli</td><td>DH5a</td><td>oriT + pSB3C5</td><td>cm</td><td>DH5a</td><td></td><td>pSB3C5</td><td> | + | </tr> |
| - | </td> | + | <tr> |
| - | </tr> | + | <td>S0048</td><td>E. coli</td><td>WM3064</td><td>SAL2_RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td> |
| - | <tr> | + | </tr> |
| - | <td>S0036</td><td>E. coli</td><td>DH5a</td><td>nah Operon + p29</td><td>amp</td><td>DH5a</td><td></td><td>BBa_J61002</td><td> | + | </table> |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0037</td><td>E. coli</td><td>WM3064</td><td>p14 RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0038</td><td>E. coli</td><td>WM3064</td><td>p16 RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0039</td><td>E. coli</td><td>WM3064</td><td>p25BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0040</td><td>E. coli</td><td>WM3064</td><td>p27BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0041</td><td>E. coli</td><td>WM3064</td><td>p29BB</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0042</td><td>E. coli</td><td>WM3064</td><td>WM_p14</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0043</td><td>E. coli</td><td>WM3064</td><td>WM_p16</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0044</td><td>E. coli</td><td>WM3064</td><td>WM_SAL1</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0045</td><td>E. coli</td><td>WM3064</td><td>p29_nah_oriT_p17c</td><td>cm</td><td>WM3064</td><td></td><td>pSB3C5</td><td>Constitutively expressed nah operon w/ oriT | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0046</td><td>E. coli</td><td>WM3064</td><td>SAL_RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0047</td><td>E. coli</td><td>WM3064</td><td>SAL2</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td><td> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td>S0048</td><td>E. coli</td><td>WM3064</td><td>SAL2_RFP</td><td>kan</td><td>WM3064</td><td></td><td>pBBRBB</td> | + | |
| - | </tr> | + | |
| - | </table | + | |
| - | + | ||
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 07:46, 26 October 2012
Strain List
| CUGEM ID | Strain Species | Strain name | Brief Description | Plasmid conferred resistances | Strain Genotype | BioBrick Insert ID | Backbone Name | Plasmid details |
|---|---|---|---|---|---|---|---|---|
| S0000 | E. coli | DH5a | N/A | N/A | N/A | N/A | ||
| S0001 | S. oneidensis | JG700 | N/A | _mtrB | N/A | N/A | N/A | |
| S0002 | S. oneidensis | JG1194 | N/A | _Mtr | N/A | N/A | N/A | |
| S0003 | S. oneidensis | JG1519 | N/A | _Mtr/_mtrB | N/A | N/A | N/A | |
| S0004 | S. oneidensis | JG1220 | kan | JG700 (S1) | N/A | ? | pmtrB | |
| S0005 | S. oneidensis | JG1219 | kan | JG700 (S1) | N/A | ? | pmtrE | |
| S0006 | S. oneidensis | JG1527 | kan | JG1519 (S3) | N/A | ? | pmtrB/mtrC/mtrA | |
| S0007 | S. oneidensis | JG1531 | kan | JG1519 (S3) | N/A | ? | pmtrE/mtrC/mtrA | |
| S0008 | S. oneidensis | JG1537 | Backbone (control) | kan | JG1519 (S3) | N/A | pBBR-BB | Empty backbone |
| S0009 | E. coli | DH5a | Failure | amp | DH5a | N/A | pBMT-1 | Gibson 1 (Failed nah operon) |
| S0010 | E. coli | DH5a | Failure | amp | DH5a | N/A | pBMT-1 | Gibson 2 (Failed nah operon) |
| S0011 | E. coli | DH5a | Failure | amp | DH5a | N/A | pBMT-1 | Gibson 3 (Failed nah operon) |
| S0012 | E. coli | DH5a | Backbone | kan | DH5a | N/A | pBBRBB-eGFP | |
| S0013 | E. coli | DH5a | ARS Reporter | kan | DH5a | ? | pBBR-BB | SP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) |
| S0014 | E. coli | DH5a | ARS Reporter | kan | DH5a | ? | pBBR-BB | SP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) |
| S0015 | E. coli | DH5a | ARS Reporter | kan | DH5a | ? | pBBR-BB | Shweta's (either SP1 or SP2?) |
| S0016 | E. coli | DH5a | ARS Reporter | kan | DH5a | ? | pBBR-BB | TS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) |
| S0017 | E. coli | DH5a | ARS Reporter | kan | DH5a | ? | pBBR-BB | TS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) |
| S0018 | E. coli | DH5a | Backbone | cm | DH5a | BBa_J04450 | pSB3C5 | mRFP1 in MCS; p15a origin; |
| S0019 | E. coli | WM3064 | Conjugation strain | N/A | DAP auxotroph | N/A | ||
| S0020a | S. oneidensis | CUGEM001 | ARS Reporter | kan | JG700 (S1) | ? | ARS Reporter w/ RBS swap (Shweta's) | |
| S0021 | P. putida | G7 | Wildtype | N/A | NAH7 | N/A | ||
| S0022 | E. coli | DH5a | Salicylate sensing | amp | DH5a | BBa_K228227 | pSB4A5 | "Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results." |
| S0023 | E. coli | DH5a | Nitrogen sensing | amp | DH5a | BBa_K216005 | pSB1A2 | "PyeaR promoter, responsive to nitrate, nitrite and nitric oxide" |
| S0024 | E. coli | DH5a | oriT | amp | DH5a | BBa_J01003 | pSB1A2 | "Origin of transfer, homologous to mob gene in PBRBB" |
| S0025 | E. coli | DH5a | Promoter | amp | DH5a | BBa_J23100 | BBa_J61002 | Constitutive promoter (1); mRFP downsteam; pMB1 origin; |
| S0026 | E. coli | DH5a | Promoter | amp | DH5a | BBa_J23101 | BBa_J61002 | Constitutive promoter (0.7); mRFP downsteam; pMB1 origin; |
| S0027 | E. coli | DH5a | Promoter | amp | DH5a | BBa_J23106 | BBa_J61002 | Constitutive promoter (0.4); mRFP downsteam; pMB1 origin; |
| S0028 | E. coli | DH5a | Promoter | amp | DH5a | BBa_J23105 | BBa_J61002 | Constitutive promoter (0.24); mRFP downsteam; pMB1 origin; |
| S0029 | E. coli | DH5a | Promoter | amp | DH5a | BBa_J23114 | BBa_J61002 | Constitutive promoter (0.1); mRFP downsteam; pMB1 origin; |
| S0030 | E. coli | DH5a | Promoter | amp | DH5a | BBa_J23103 | BBa_J61002 | Constitutive promoter (0.01); mRFP downsteam; pMB1 origin; |
| S0031 | E. coli | DH5a | Backbone | cm | DH5a | BBa_J04450 | pSB1C3 | mRFP1 in MCS; pMB1 origin; |
| S0032 | E. coli | DH5a | nah Operon | cm | DH5a | pSB1C3 | nah operon in MCS; pBM1 origin; | |
| S0033 | E. coli | DH5a | lac inducible mtrB | kan | DH5a | BBa_K098984 | pSB3K3 | lac indubible mtrB in MCS; p15a origin; |
| S0034 | E. coli | DH5a | SAL (Salicylate Reporter) | kan | DH5a | pBBR-BB | ||
| S0035 | E. coli | DH5a | oriT + pSB3C5 | cm | DH5a | pSB3C5 | ||
| S0036 | E. coli | DH5a | nah Operon + p29 | amp | DH5a | BBa_J61002 | ||
| S0037 | E. coli | WM3064 | p14 RFP | kan | WM3064 | pBBRBB | ||
| S0038 | E. coli | WM3064 | p16 RFP | kan | WM3064 | pBBRBB | ||
| S0039 | E. coli | WM3064 | p25BB | kan | WM3064 | pBBRBB | ||
| S0040 | E. coli | WM3064 | p27BB | kan | WM3064 | pBBRBB | ||
| S0041 | E. coli | WM3064 | p29BB | kan | WM3064 | pBBRBB | ||
| S0042 | E. coli | WM3064 | WM_p14 | kan | WM3064 | pBBRBB | ||
| S0043 | E. coli | WM3064 | WM_p16 | kan | WM3064 | pBBRBB | ||
| S0044 | E. coli | WM3064 | WM_SAL1 | kan | WM3064 | pBBRBB | ||
| S0045 | E. coli | WM3064 | p29_nah_oriT_p17c | cm | WM3064 | pSB3C5 | Constitutively expressed nah operon w/ oriT | |
| S0046 | E. coli | WM3064 | SAL_RFP | kan | WM3064 | pBBRBB | ||
| S0047 | E. coli | WM3064 | SAL2 | kan | WM3064 | pBBRBB | ||
| S0048 | E. coli | WM3064 | SAL2_RFP | kan | WM3064 | pBBRBB |
