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| | Improvements and testing of existing biobricks </h2><!-- .postitle --> | | Improvements and testing of existing biobricks </h2><!-- .postitle --> |
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| - | <p><span style="color:#000000;">Based on the data displayed <a href="http://partsregistry.org/Part:BBa_K398108:Experience#User_Reviews"><span style="color:#000000;">here</span></a>, we have determined the conditions in which bacteria display the most tumbling. Tumbling is a chemotactic response to environmental conditions that signifies a positive bacterial response. At a salt concentration of 0.5 M, an average of ~10% of bacteria were consistently tumbling. There is a clear bell-curve trend of induced tumbling in <em>E. coli</em> that contain the BBa_K398108 salt resistance with optimal conditions at 0.5M NaCl. </span></p> | + | <p><strong>BBa_K283030 and BBa_I712074</strong></p> |
| | + | <p>We have placed an T7 promoter BBa_I712074 upstream of the RBS-<em>cheZ</em> BBa_K283030 system making it a functional biobrick for inducible expression in an <em>E. coli</em> host harboring the lambda DE3 lysogen. We have tested this biobrick for functionality and confirmed that it works as expected. |
| | + | <br> |
| | + | <p><strong>BBa_K398108</strong></p> |
| | + | <p><span style="color:#000000;">Based on the data displayed <a href="http://partsregistry.org/Part:BBa_K398108:Experience#User_Reviews"><span style="color:#000000;">here</span></a>, we have determined the conditions in which bacteria display the most tumbling. Tumbling is a chemotactic response to environmental conditions that signifies a positive bacterial response. At a salt concentration of 0.5 M, an average of ~10% of bacteria were consistently tumbling. There is a clear bell-curve trend of induced tumbling in <em>E. coli</em> that contain the BBa_K398108 salt resistance with optimal conditions at 0.5M NaCl. </span></p> |
| | <p><span style="color:#000000;"><a href="http://uscigem2012.files.wordpress.com/2012/10/screen-shot-2012-10-03-at-4-13-37-pm.png"><span style="color:#000000;"><img class="aligncenter size-full wp-image-280" title="Screen Shot 2012-10-03 at 4.13.37 PM" src="http://uscigem2012.files.wordpress.com/2012/10/screen-shot-2012-10-03-at-4-13-37-pm.png?w=960" alt="" /></span></a> </span></p> | | <p><span style="color:#000000;"><a href="http://uscigem2012.files.wordpress.com/2012/10/screen-shot-2012-10-03-at-4-13-37-pm.png"><span style="color:#000000;"><img class="aligncenter size-full wp-image-280" title="Screen Shot 2012-10-03 at 4.13.37 PM" src="http://uscigem2012.files.wordpress.com/2012/10/screen-shot-2012-10-03-at-4-13-37-pm.png?w=960" alt="" /></span></a> </span></p> |
| | <p><span style="color:#000000;">This is a congruent with the data found by the TU Delft iGEM 2010 team. A peak growth rate can be observed at 0.5 M NaCl, signifying positive cellular conditions. Our data also suggests optimal NaCl/environmental conditions at this concentration.</span></p> | | <p><span style="color:#000000;">This is a congruent with the data found by the TU Delft iGEM 2010 team. A peak growth rate can be observed at 0.5 M NaCl, signifying positive cellular conditions. Our data also suggests optimal NaCl/environmental conditions at this concentration.</span></p> |
Improvements and testing of existing biobricks
BBa_K283030 and BBa_I712074
We have placed an T7 promoter BBa_I712074 upstream of the RBS-cheZ BBa_K283030 system making it a functional biobrick for inducible expression in an E. coli host harboring the lambda DE3 lysogen. We have tested this biobrick for functionality and confirmed that it works as expected.
BBa_K398108
Based on the data displayed here, we have determined the conditions in which bacteria display the most tumbling. Tumbling is a chemotactic response to environmental conditions that signifies a positive bacterial response. At a salt concentration of 0.5 M, an average of ~10% of bacteria were consistently tumbling. There is a clear bell-curve trend of induced tumbling in E. coli that contain the BBa_K398108 salt resistance with optimal conditions at 0.5M NaCl.
This is a congruent with the data found by the TU Delft iGEM 2010 team. A peak growth rate can be observed at 0.5 M NaCl, signifying positive cellular conditions. Our data also suggests optimal NaCl/environmental conditions at this concentration.
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