Team:Michigan/Notebook/Protocols

From 2012.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 8: Line 8:
</h3>
</h3>
<h2>General</h2>
<h2>General</h2>
-
</html>
+
Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html>
-
Plate Production
+
 
<html>
<html>
<h2>Cloning</h2>
<h2>Cloning</h2>
-
</html>
 
-
Removing BioBricks from the Registry Wells[[File:MichRem.pdf]]
 
-
Production of Competent Cells[[File:MichComp.pdf]]
 
-
Heat Shock Transformation
+
Removing BioBricks from the Registry</html>[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]<html>
-
DNA Purification
+
<br><br>
 +
Production of Competent Cells</html>[https://static.igem.org/mediawiki/2012/6/61/MichComp.pdf]<html>
 +
<br><br>
-
Restriction Enzyme Digest
+
Heat Shock Transformation</html>[https://static.igem.org/mediawiki/2012/0/0e/MichHeat.pdf]<html>
-
Ligation
+
<br><br>
-
PCR (Phusion)
+
DNA Purification: PCR</html>[https://static.igem.org/mediawiki/2012/0/04/MichPur2.pdf]<html>
 +
DNA Purification: MiniPrep</html>[https://static.igem.org/mediawiki/2012/1/19/MichPur1.pdf]<html>
 +
<br><br>
-
Colony PCR
+
Restriction Enzyme Digest</html>[https://static.igem.org/mediawiki/2012/0/0f/MichRestrict.pdf]<html>
 +
 
 +
<br><br>
 +
 
 +
Ligation</html>[https://static.igem.org/mediawiki/2012/1/10/MichLig.pdf]<html>
 +
 
 +
<br><br>
 +
 
 +
PCR (Phusion)</html>[https://static.igem.org/mediawiki/2012/9/94/MichPCR.pdf]<html>
 +
 
 +
<br><br>
 +
 
 +
Colony PCR</html>[https://static.igem.org/mediawiki/2012/9/96/MichCol.pdf]<html>
 +
 
 +
<br><br>
 +
 
 +
Gel Electrophoresis</html>[https://static.igem.org/mediawiki/2012/1/12/MichElectro.pdf]<html>
 +
 
 +
<br><br>
-
<html>
 
<h2>Characterization</h2>
<h2>Characterization</h2>
-
</html>
+
<br>
-
Transmission Electron Microscopy Protocol
+
Transmission Electron Microscopy Protocol</html>[https://static.igem.org/mediawiki/2012/0/0a/MichTEM.pdf]<html>
 +
<br><br>
 +
FimE Asymmetrical Digest Assay Protocol</html>[https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf]<html>
-
FimE Asymmetrical Digest Assay Protocol
 
-
<html>
 
</div>
</div>
</div>
</div>
</html>
</html>

Latest revision as of 02:26, 4 October 2012

Xayona Website Template

Protocols

General

Plate Production[1]

Cloning

Removing BioBricks from the Registry[2]

Production of Competent Cells[3]

Heat Shock Transformation[4]

DNA Purification: PCR[5] DNA Purification: MiniPrep[6]

Restriction Enzyme Digest[7]

Ligation[8]

PCR (Phusion)[9]

Colony PCR[10]

Gel Electrophoresis[11]

Characterization


Transmission Electron Microscopy Protocol[12]

FimE Asymmetrical Digest Assay Protocol[13]