"
Team:University College London/Week12YanikaExp6
From 2012.igem.org
(Difference between revisions)
YanikaBorg (Talk | contribs) (Created page with "Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four con...") |
YanikaBorg (Talk | contribs) |
||
| (One intermediate revision not shown) | |||
| Line 4: | Line 4: | ||
{| class ="bigtable" | {| class ="bigtable" | ||
|- | |- | ||
| - | ! !! !! | + | ! !! LIGATIONS: !! 1 !! 2 !! 4 !! 5 !! CONTROLS !! 1 !! 2 !! 4 !! 5 |
|- | |- | ||
| - | | | + | |dH2O || || 3.5 || 3.5 || 3.5 || 3.5 || || 4.5 || 4.5 || 4.5 || 4.5 |
|- | |- | ||
| - | | | + | |NEB || || 1 || 1 || 1 || 1 || || 1 || 1 || 1 || 1 |
|- | |- | ||
| - | | | + | |DNA || || 3 || 3 || 3 || 3 || || 3 || 3 || 3 || 3 |
|- | |- | ||
| - | | | + | |BSA || || 0.5 || 0.5 || 0.5 || 0.5 || || 0.5 || 0.5 || 0.5 || 0.5 |
|- | |- | ||
| - | | | + | |XBal || || 1 || 1 || 1 || 1 || || 1 || 1 || 1 || 1 |
|- | |- | ||
| - | | | + | |SpeI || || 1 || 1 || 1 || 1 || || 0 || 0 || 0 || 0 |
|- | |- | ||
| - | | | + | |TOTAL || || 10 || 10 || 10 || 10 || || 10 || 10 || 10 || 10 |
| - | + | ||
| - | + | ||
|} | |} | ||
Latest revision as of 22:06, 26 September 2012
Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four controls were used for each digest. A sample was cut with one enzyme in order to linearise the DNA.
| LIGATIONS: | 1 | 2 | 4 | 5 | CONTROLS | 1 | 2 | 4 | 5 | |
|---|---|---|---|---|---|---|---|---|---|---|
| dH2O | 3.5 | 3.5 | 3.5 | 3.5 | 4.5 | 4.5 | 4.5 | 4.5 | ||
| NEB | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| DNA | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | ||
| BSA | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | ||
| XBal | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| SpeI | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | ||
| TOTAL | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
