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Team:University College London/Week12YanikaExp6
From 2012.igem.org
Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four controls were used for each digest. A sample was cut with one enzyme in order to linearise the DNA.
| LIGATIONS: | 1 | 2 | 4 | 5 | CONTROLS | 1 | 2 | 4 | 5 | |
|---|---|---|---|---|---|---|---|---|---|---|
| dH2O | 3.5 | 3.5 | 3.5 | 3.5 | 4.5 | 4.5 | 4.5 | 4.5 | ||
| NEB | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| DNA | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | ||
| BSA | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | ||
| XBal | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| SpeI | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | ||
| TOTAL | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
