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Team:TU Darmstadt/Materials/Materials
From 2012.igem.org
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=== CARL ROTH GMBH + CO. KG === | === CARL ROTH GMBH + CO. KG === | ||
* acetic acid | * acetic acid | ||
| + | * acetone | ||
* acrylamide (rotiphorese) | * acrylamide (rotiphorese) | ||
* agar-agar kobe I | * agar-agar kobe I | ||
| Line 50: | Line 51: | ||
* ethanol | * ethanol | ||
* ethylenediaminetetraacetic acid (EDTA) | * ethylenediaminetetraacetic acid (EDTA) | ||
| + | * ethylacetate | ||
* gloves | * gloves | ||
* glycerol | * glycerol | ||
* HCl | * HCl | ||
| + | * hexane | ||
* imidazole | * imidazole | ||
* isopropyl alcohol | * isopropyl alcohol | ||
| Line 59: | Line 62: | ||
* KH<sub>2</sub>PO<sub>4</sub> | * KH<sub>2</sub>PO<sub>4</sub> | ||
* L-(+)-arabinose | * L-(+)-arabinose | ||
| + | * methanol | ||
* Na<sub>2</sub>HPO<sub>4</sub> | * Na<sub>2</sub>HPO<sub>4</sub> | ||
* NaCl | * NaCl | ||
| Line 64: | Line 68: | ||
* NiCl<sub>2</sub> | * NiCl<sub>2</sub> | ||
* sodiumdodecylsulfate (SDS) | * sodiumdodecylsulfate (SDS) | ||
| + | * sulfuric acid 96% (w/v) | ||
* tributyrine | * tributyrine | ||
* tricine | * tricine | ||
| + | * triethylamine | ||
* TRIS-HCl | * TRIS-HCl | ||
* tryptone | * tryptone | ||
* yeast extract | * yeast extract | ||
| + | |||
=== AppliChem GmbH === | === AppliChem GmbH === | ||
* bromothymol blue | * bromothymol blue | ||
| Line 75: | Line 82: | ||
=== Sigma-Aldrich Co. LLC. === | === Sigma-Aldrich Co. LLC. === | ||
* bromphenol blue | * bromphenol blue | ||
| + | * dimethylterephtalate | ||
* hydrocinnamoyl chloride | * hydrocinnamoyl chloride | ||
* para-nitrophenol | * para-nitrophenol | ||
Latest revision as of 21:10, 26 September 2012
Contents |
Chemicals
CARL ROTH GMBH + CO. KG
- acetic acid
- acetone
- acrylamide (rotiphorese)
- agar-agar kobe I
- ammonium persulfate (APS)
- ammonium sulfate
- ampicillin
- ß-mercaptoethanol
- chloramphenicol
- coomassie brillant blue 250
- D-(+)-glucose
- ethanol
- ethylenediaminetetraacetic acid (EDTA)
- ethylacetate
- gloves
- glycerol
- HCl
- hexane
- imidazole
- isopropyl alcohol
- kanmycin
- KCl
- KH2PO4
- L-(+)-arabinose
- methanol
- Na2HPO4
- NaCl
- NaOH
- NiCl2
- sodiumdodecylsulfate (SDS)
- sulfuric acid 96% (w/v)
- tributyrine
- tricine
- triethylamine
- TRIS-HCl
- tryptone
- yeast extract
AppliChem GmbH
- bromothymol blue
- tetramethylethylenediamine (TEMED)
Sigma-Aldrich Co. LLC.
- bromphenol blue
- dimethylterephtalate
- hydrocinnamoyl chloride
- para-nitrophenol
- succinyl chloride
- tween 20
New England Biolabs GmbH
Enzymes
- EcoRI
- EcoRI HF
- NcoI HF
- NdeI HF
- NotI HF
- Phusion
- PstI HF
- Q5 Polymerase Hotstart
- Q5 Polymerase normal
- T4 DNA Ligase
- XbaI HF
Buffer
- 2-log DNA marker
- bovine serum albumin (BSA 100x)
- Buffer 4
- dimethylsulfoxide (DMSO)
- DNA loading Dye 6x
- GC Phusion 5x Buffer
- GC Q5 Enhancer Buffer
- MgCl2
- Phusion HF Buffer 5x
- Q5 reaction Buffer
- T4 Ligase Buffer 10x
Promega GmbH
Enzymes
- EcoRI
- PstI
- SpeI GQ
- T4 Ligase
- TEV Protease
- XbaI GQ
Buffer
- Buffer B
- Buffer D
- Buffer H
- Go Tag Puffer 5x
- Go Tag Puffer 5x prestained
- Multi Core 10x Buffer
- T4 Ligase Buffer 10x
Kits
- Pure Yield Plasmid Miniprep System
- Wizard SV Gel and PCR Clean Up System
