"
Team:Evry/AuxinTOX
From 2012.igem.org
| (4 intermediate revisions not shown) | |||
| Line 6: | Line 6: | ||
<h2> The purpose of this experiment </h2> | <h2> The purpose of this experiment </h2> | ||
| - | <p>Before testing | + | <p>Before testing hormonal system in embryos (see <a href="https://2012.igem.org/Team:Evry/AIDSystem">here</a>), we conducted auxin toxicity test. Auxin concentrations used were 500, 250, 125 and 0 µM dissolved in MMR, with renewal of the medium each morning. (see medium preparation <a href=" https://2012.igem.org/Team:Evry/Protocols#Auxin_toxicity_test"> here </a>) </b></p> |
<h2>Result interpretation:</h2><br> | <h2>Result interpretation:</h2><br> | ||
| - | <p>The survival rate of embryos in both 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) were observed during three days of experiment. <br> | + | <p>The survival rate of embryos in both 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) were observed in triplicate during three days of experiment. <br> |
As a control sample, embryos were placed in MMR 0.1x medium without an auxin.<br/> | As a control sample, embryos were placed in MMR 0.1x medium without an auxin.<br/> | ||
| + | The initial number of embryos for each medium condition was superior than 40.<br> | ||
The embryo's mortality rate was about:<br> | The embryo's mortality rate was about:<br> | ||
| - | <b>for NAA</b> 23% for D1 and 45% for D2 <br> | + | <b>for NAA</b> 23% for day 1 (D1) and 45% for day 2 (D2) <br> |
<b>for IAA</b> 20% for D1 and 51% for D2 <br> | <b>for IAA</b> 20% for D1 and 51% for D2 <br> | ||
<br> | <br> | ||
| Line 30: | Line 31: | ||
<i>Control sample- embryos in MMR medium</i><br> | <i>Control sample- embryos in MMR medium</i><br> | ||
<br> | <br> | ||
| - | <center><img src="https://static.igem.org/mediawiki/2012/8/8c/Naa_control.jpg" alt="" width=" | + | <center><img src="https://static.igem.org/mediawiki/2012/8/8c/Naa_control.jpg" alt="" width="350px" /></center><br> |
<i>Embryos in MMR medium with 250µM NAA</i><br> | <i>Embryos in MMR medium with 250µM NAA</i><br> | ||
<br> | <br> | ||
| - | <center><img src="https://static.igem.org/mediawiki/2012/3/3a/Tadpole_naa.jpg" alt="" width=" | + | <center><img src="https://static.igem.org/mediawiki/2012/3/3a/Tadpole_naa.jpg" alt="" width="350px" /></center><br> |
Latest revision as of 02:19, 27 September 2012
Auxin toxicity test in tadpoles
The purpose of this experiment
Before testing hormonal system in embryos (see here), we conducted auxin toxicity test. Auxin concentrations used were 500, 250, 125 and 0 µM dissolved in MMR, with renewal of the medium each morning. (see medium preparation here )
Result interpretation:
The survival rate of embryos in both 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) were observed in triplicate during three days of experiment.
As a control sample, embryos were placed in MMR 0.1x medium without an auxin.
The initial number of embryos for each medium condition was superior than 40.
The embryo's mortality rate was about:
for NAA 23% for day 1 (D1) and 45% for day 2 (D2)
for IAA 20% for D1 and 51% for D2
However embryos mortality depends not only on the auxin presence in media, but also on the rearing conditions, frequency of sorting dead and live embryos and media's changing. Usually the normal mortality ratio is no bigger than 30%.
We are aware that our experiment should be repeated to calculate the error deviation. But even without this data we confirmed our hypothesis that presence of auxin in MMR medium is not toxic for embryos and it doesn't cause any morphological abnormalities.
Data:
Control sample- embryos in MMR medium
Embryos in MMR medium with 250µM NAA
