Team:Marburg SYNMIKRO/Primer

From 2012.igem.org

(Difference between revisions)

Latest revision as of 23:33, 26 September 2012

Primer

No.	Name	Sequence	Length	G/C content	TM
MS1	AmiC_ss_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'	38 bp	55.2 %	86.5 °C
MS2	AmiC_ss_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'	38 bp	63.1 %	85.5 °C
MS3	HU_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'	37 bp	51.3 %	83.6 °C
MS4	HU_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv	38 bp	57.8 %	82.8 °C
MS4b	HU_std_10_rev2	5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'	45 bp	57.7 %	85.7 °C
MS5	GroEs_std_10_fwd	5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'	120 bp	51.6 %	98.0 °C
MS6	GroEs_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'	45 bp	57.7 %	88.7 °C
MS6b	GroES_std_10_rev2	5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'	38 bp	52.6 %	81.4 °C
MS7	GixR_std_10_fwd	5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'	54 bp	50.0 %	85.1 °C
MS8	GixR_std_10_rev	5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'	46 bp	50.0 %	82.7 °C
MS9	Enhancer_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'	42 bp	54.7 %	86.2 °C
MS10	Enhancer_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'	41 bp	56.0 %	85.1 °C
MS11	GIN_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'	52 bp	50.0 %	87.6 °C
MS12	RBS + GIN_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'	72 bp	48.8 %	89.8 °C
MS13	GIN_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'	59 bp	45.7 %	85.5 °C
MS14	RFP_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'	41 bp	58.5 %	87.6 °C
MS15	RFP_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'	41 bp	56.0 %	84.2 °C
MS16	CFP_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'	43 bp	62.7 %	90.3 °C
MS17	CFP_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'	43 bp	51.1 %	80.5 °C
MS18	Prefix_std_10_fwd	5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'	28 bp	53.5 %	77.0 °C
MS19	Suffix_std_10_rev	5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'	29 bp	55.1 %	75.3 °C
MS20	VF2_std_10_fwd	5'-TGCCACCTGACGTCTAAGAA-3'	20 bp	50.0 %	63.5 °C
MS21	VR_std_10_rev	5'-ATTACCGCCTTTGAGTGAGC-3'	20 bp	50 %	63.1 °C
MS22	SB-prep-3P-1	5'-GCCGCTGCAGTCCGGCAAAAAA-3'	22 bp	59.0 %	77.2 °C
MS23	SB-prep-2Ea	5'-ATGAATTCCAGAAATCATCCTTAGCG-3'	26 bp	38.4 %	67.6 °C
MS24	Suffix_std_10_fwd	5'-ACTAGTAGCGGCCGCTGCAG-3'	20 bp	65.0 %	68.8 °C
MS25	Prefix_std_10_rev	5'-TCTAGATGCGGCCGCGAATTC-3'	21 bp	57.1 %	72.2 °C
MS26	SacB_std_10_fwd	5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'	42 bp	47.6 %	84.8 °C
MS27	SacB_std_10_rev	5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'	40 bp	45.0 %	76.8 °C

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-==Primer==
-{|class="wikitable" align=center
+<html>
+<div class="conboxb">
+</html>
+===Primer===
+{| class="wikitable"
 |-
 !No.!!Name!!Sequence!!Length!!G/C content!!TM
 |-
-|<div id="MS1"></div>MS1||AmiC_ss_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'||xx bp||yy %||zz °C
+|<div id="MS1"></div>MS1||AmiC_ss_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'||38 bp||55.2 %||86.5 °C
 |-
-|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C
+|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||38 bp||63.1 %||85.5 °C
 |-
-|<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'||xx bp||yy %||zz °C
+|<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'||37 bp||51.3 %||83.6 °C
 |-
-|<div id="MS4"></div>MS4||HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv||xx bp||yy %||zz °C
+|<div id="MS4"></div>MS4||HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv||38 bp||57.8 %||82.8 °C
 |-
-|<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'||xx bp||yy %||zz °C
+|<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'||45 bp||57.7 %||85.7 °C
 |-
-|<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'||xx bp||yy %||zz °C
+|<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'||120 bp||51.6 %||98.0 °C
 |-
-|<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'||xx bp||yy %||zz °C
+|<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'||45 bp||57.7 %||88.7 °C
 |-
-|<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'||xx bp||yy %||zz °C
+|<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'||38 bp||52.6 %||81.4 °C
 |-
-|<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'||xx bp||yy %||zz °C
+|<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'||54 bp||50.0 %||85.1 °C
 |-
-|<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'||xx bp||yy %||zz °C
+|<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'||46 bp||50.0 %||82.7 °C
 |-
-|<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'||xx bp||yy %||zz °C
+|<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'||42 bp||54.7 %||86.2 °C
 |-
-|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'||xx bp||yy %||zz °C
+|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'||41 bp||56.0 %||85.1 °C
 |-
 |<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||52 bp||50.0 %||87.6 °C
@@ Line 132: / Line 136: @@
 |}
+<html>
+</div>
+</html>
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