Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

(Difference between revisions)
(→Strain Database)
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Line 48: Line 48:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM001
+
| pIGM001
| LasR Promoter
| LasR Promoter
| [http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
| [http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
Line 57: Line 57:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM002
+
| pIGM002
| LasR
| LasR
| [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
| [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
Line 66: Line 66:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM003
+
| pIGM003
| Lac Promoter
| Lac Promoter
| [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
| [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
Line 75: Line 75:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM004
+
| pIGM004
| GFP
| GFP
| [http://partsregistry.org/Part:BBa_I714891 BBa_I714891]
| [http://partsregistry.org/Part:BBa_I714891 BBa_I714891]
Line 84: Line 84:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM005
+
| pIGM005
-
| <span style="text-decoration:line-through;">RFP</span> (use IGM021 instead)
+
| RFP
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
| pSB1AK3
| pSB1AK3
Line 93: Line 93:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM006
+
| pIGM006
| LasI
| LasI
| [http://partsregistry.org/Part:BBa_K081009 BBa_K081009]
| [http://partsregistry.org/Part:BBa_K081009 BBa_K081009]
Line 102: Line 102:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM007
+
| pIGM007
| RBS + LacI
| RBS + LacI
| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
| pSB1A2
| pSB1A2
| 2012 kit plate 1 (7L)
| 2012 kit plate 1 (7L)
-
|
+
| Verified
| Aurko
| Aurko
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM008
+
| pIGM008
| Double terminator (BBa_B0010 - BBa_B0012)
| Double terminator (BBa_B0010 - BBa_B0012)
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
| pSB1AK3
| pSB1AK3
| 2012 kit plate 1 (23L)
| 2012 kit plate 1 (23L)
-
|
+
| Verified
| Aurko
| Aurko
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM009
+
| pIGM009
| J23100 promoter - sfGFP (Part:BBa_K515105)
| J23100 promoter - sfGFP (Part:BBa_K515105)
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105]
Line 129: Line 129:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM014
+
| pIGM014
| Plasmid pSB1A3 backbone with RFP Insert
| Plasmid pSB1A3 backbone with RFP Insert
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
Line 138: Line 138:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM015
+
| pIGM015
| Plasmid pSB1C3 backbone with RFP Insert
| Plasmid pSB1C3 backbone with RFP Insert
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
Line 147: Line 147:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM016
+
| pIGM016
| Plasmid pSB1K3 backbone with RFP Insert
| Plasmid pSB1K3 backbone with RFP Insert
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
Line 156: Line 156:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM017
+
| pIGM017
| Plasmid pSB1T3 backbone with RFP Insert
| Plasmid pSB1T3 backbone with RFP Insert
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
Line 165: Line 165:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM021
+
| pIGM021
| RFP
| RFP
| [http://partsregistry.org/Part:BBa_J06504 BBa_J06504]
| [http://partsregistry.org/Part:BBa_J06504 BBa_J06504]
| pSB1A2
| pSB1A2
| 2012 kit plate 1 (13F)
| 2012 kit plate 1 (13F)
-
| Incorrect
+
| '''Incorrect'''
| Ben
| Ben
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM022
+
| pIGM022
|  
|  
|  
|  
Line 183: Line 183:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM023
+
| pIGM023
|  
|  
|  
|  
Line 192: Line 192:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM024
+
| pIGM024
|  
|  
|  
|  
Line 201: Line 201:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM025
+
| pIGM025
|  
|  
|  
|  
Line 210: Line 210:
|-
|-
| Plasmid Box 1
| Plasmid Box 1
-
| IGM026
+
| pIGM026
| sfGFP
| sfGFP
|  
|  
Line 217: Line 217:
|
|
| Razan
| Razan
 +
|-
 +
| Plasmid Box 1
 +
| pIGM027
 +
| Promoter-Theophylline riboswitch 1-Venus-Double terminator
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537009 BBa_K537009]
 +
| pSB1C3
 +
| 2012 kit plate 5 (2M)
 +
| '''Incorrect'''
 +
| Ben
 +
|-
 +
| Plasmid Box 1
 +
| pIGM028
 +
| Theophylline Riboswitch 1-Venus
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537003 BBa_K537003]
 +
| pSB1C3
 +
| 2012 kit plate 5 (7I)
 +
|
 +
| Peter/Ben
|}
|}
Line 273: Line 291:
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
-
| 7
+
|  
 +
| IGM004
 +
| pSB3K3-I714891 (GFP -  but not used in construct)
 +
| TOP10
 +
| Barrick Lab
 +
| Ben
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM005
 +
| pSB1AK3-K081014 (RFP)
 +
| TOP10
 +
| Barrick Lab
 +
| Ben
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM006
 +
| pSB1A2-K081009 (LasI)
 +
| TOP10
 +
| Barrick Lab
 +
| Ben
 +
|-
 +
| iGEM 2012 Box 1
 +
|
| IGM009
| IGM009
| crdASC (4864 bp)
| crdASC (4864 bp)
Line 281: Line 323:
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
-
| 8
+
|  
| IGM010
| IGM010
| crdASC (4943 bp)
| crdASC (4943 bp)
Line 289: Line 331:
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
-
| 9
+
|  
| IGM011
| IGM011
| crdASC (4864 bp)
| crdASC (4864 bp)
Line 297: Line 339:
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
-
| 10
+
|  
| IGM012
| IGM012
| crdASC (4943 bp)
| crdASC (4943 bp)
Line 383: Line 425:
| Parts Kit
| Parts Kit
| Erik
| Erik
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM023
 +
| pet21.KOD
 +
| TOP10
 +
| Jared
 +
|
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM024
 +
| pQL269
 +
| TOP10
 +
| Jared
 +
|
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM025
 +
| pACYC.T7.GFP
 +
| TOP10
 +
| Jared
 +
|
|-
|-
| iGEM 2012 Box 1
| iGEM 2012 Box 1
Line 392: Line 458:
| Ben
| Ben
|-
|-
-
|}
+
| iGEM 2012 Box 1
-
 
+
|
-
= ZombiE.coli parts =
+
| IGM027
-
 
+
| pAK.TAQ
-
{| cellpadding="5" border="1" cellspacing="0"
+
| TOP10
-
! Plasmid
+
| Jared
-
! Part
+
|
-
! Description
+
-
! Source
+
|-
|-
-
| pReporter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_I714891 BBa_I714891]
+
|  
-
| GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'')
+
| IGM028
-
| 2012 kit plate 2 (15L)
+
| PAAV-miniCMV-mCherry
 +
| Bl-21 AI
 +
| Addgene
 +
| Logan
|-
|-
-
| pReporter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
+
|  
-
| RFP protein generator - PoPS->RFP
+
| IGM029
-
| 2012 kit plate 3 (12O)
+
| pET-SpA_ScTrp
 +
| Bl-21 DE3
 +
|  
 +
| Logan
|-
|-
-
| pReporter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_K081009 BBa_K081009]
+
|  
-
| LasI protein generator (TERM-)
+
| IGM030
-
| 2012 kit plate 2 (10N)
+
| pET-SpA_ScTrp_mCherry_5'
 +
| Bl-21 AI
 +
|
 +
| Logan
|-
|-
-
| pReporter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
+
|  
-
| RBS + LacI
+
| IGM031
-
| 2012 kit plate 1 (7L)
+
| pET-SpA_ScTrp_mCherry_5'
 +
| Bl-21 DE3
 +
|
 +
| Logan
|-
|-
-
| pReporter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
+
|  
-
| Double terminator (BBa_B0010-BBa_B0012)
+
| IGM032
-
| 2012 kit plate 1 (23L)
+
| pET-SpA_ScTrp_mCherry_3'
 +
| Bl-21 DE3
 +
|
 +
| Logan
|-
|-
-
| pConverter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
+
|  
-
| LasR Promoter
+
| IGM033
-
| 2012 kit plate 1 (12A)
+
| pET-SpA_ScTrp
 +
| Bl-21 DE3
 +
|
 +
| Logan
|-
|-
-
| pConverter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
+
|  
-
| LasR
+
| IGM034
-
| 2012 kit plate 1 (14J)
+
| pAAV-miniCMV-mCherry
 +
| Bl-21 DE3
 +
|
 +
| Logan
|-
|-
-
| pConverter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_K082016 BBa_K082016]
+
|  
-
| LacIM1
+
| IGM035
-
| Parts Registry
+
| psb3k3-J2315.ORF1 + pra301-ndmApro
 +
| TOP10
 +
| Barrick Lab
 +
| Peter
|-
|-
-
| pConverter
+
| iGEM 2012 Box 1
-
| [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
+
|  
-
| LacI Promoter
+
| IGM036
-
| 2012 kit plate 1 (1D)
+
| J23001-NdmABCDgst9-pSB1C3
 +
| BW25113-guaB
 +
| Erik
 +
| Erik
|-
|-
-
| pConverter
+
| iGEM 2012 Box 1
|  
|  
-
| CRE
+
| IGM037
-
| pQL269 Plasmid
+
| psb3k3-J2315.ORF1
-
 
+
| TOP10
 +
| Barrick Lab
 +
| Peter
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM038
 +
| none
 +
| BW25113-guaB
 +
| Erik
 +
| Erik
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM039
 +
| pSB1C3-K537011
 +
| TOP10
 +
| iGEM parts kit
 +
| Peter
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM040
 +
| pSB1C3-K537003
 +
| TOP10
 +
| iGEM parts kit
 +
| Peter
 +
|-
 +
| iGEM 2012 Box 1
 +
|
 +
| IGM041
 +
| NdmABCD
 +
| BW25113-guaB
 +
| Erik
 +
| Razan
|}
|}

Latest revision as of 20:22, 11 December 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Label Name Part Plasmid Source Sequence Added by
Plasmid Box 1 cadA ASKA cadA plasmid Barrick Lab
Plasmid Box 1 ldcC ASKA ldcC plasmid Barrick Lab
Plasmid Box 1 pIGM001 LasR Promoter [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] pSB1A2 2012 kit plate 1 (12A) Verified Razan
Plasmid Box 1 pIGM002 LasR [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] pSB3K3 2012 kit plate 1 (14J) Verified Razan
Plasmid Box 1 pIGM003 Lac Promoter [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] pSB1A2 2012 kit plate 1 (1D) Razan
Plasmid Box 1 pIGM004 GFP [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] pSB3K3 2012 kit plate 2 (15L) Verified Ben
Plasmid Box 1 pIGM005 RFP [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] pSB1AK3 2012 kit plate 3 (12O) Verified Ben
Plasmid Box 1 pIGM006 LasI [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] pSB1A2 2012 kit plate 2 (10N) Verified Ben
Plasmid Box 1 pIGM007 RBS + LacI [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] pSB1A2 2012 kit plate 1 (7L) Verified Aurko
Plasmid Box 1 pIGM008 Double terminator (BBa_B0010 - BBa_B0012) [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] pSB1AK3 2012 kit plate 1 (23L) Verified Aurko
Plasmid Box 1 pIGM009 J23100 promoter - sfGFP (Part:BBa_K515105) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] pSB1C3 2012 kit plate 5 (1K)
Plasmid Box 1 pIGM014 Plasmid pSB1A3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1A3 2012 kit plate 1 (1G) Logan
Plasmid Box 1 pIGM015 Plasmid pSB1C3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1C3 2012 kit plate 1 (3A) Logan
Plasmid Box 1 pIGM016 Plasmid pSB1K3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1K3 2012 kit plate 1 (5A) Logan
Plasmid Box 1 pIGM017 Plasmid pSB1T3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1T3 2012 kit plate 1 (7A) Logan
Plasmid Box 1 pIGM021 RFP [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] pSB1A2 2012 kit plate 1 (13F) Incorrect Ben
Plasmid Box 1 pIGM022 pAK.TAQ Jared Mike
Plasmid Box 1 pIGM023 pET21.KOD Jared Mike
Plasmid Box 1 pIGM024 pQL269 Jared Mike
Plasmid Box 1 pIGM025 pACYC.T7.GFP Jared Mike
Plasmid Box 1 pIGM026 sfGFP pET21 Razan
Plasmid Box 1 pIGM027 Promoter-Theophylline riboswitch 1-Venus-Double terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537009 BBa_K537009] pSB1C3 2012 kit plate 5 (2M) Incorrect Ben
Plasmid Box 1 pIGM028 Theophylline Riboswitch 1-Venus [http://partsregistry.org/wiki/index.php?title=Part:BBa_K537003 BBa_K537003] pSB1C3 2012 kit plate 5 (7I) Peter/Ben

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Label Description Strain Source Added by
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab
iGEM 2012 Box 1 IGM004 pSB3K3-I714891 (GFP - but not used in construct) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM005 pSB1AK3-K081014 (RFP) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM006 pSB1A2-K081009 (LasI) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM009 crdASC (4864 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM010 crdASC (4943 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 IGM011 crdASC (4864 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 IGM012 crdASC (4943 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 11 IGM013 BBa_K515105: J23100-sfGFP-pSB1C3(CamR) epi300 Parts Kit
iGEM 2012 Box 1 IGM014 pSB1A3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM015 pSB1C3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM016 pSB1K3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM017 pSB1T3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM018 pSB1A2 with LasR Promoter Bba_R0079 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM019 pSB2K3 with LasR Bba_C0079 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM020 pSB1A2 with Lac Promoter Bba_R0010 TOP10 Parts Kit Razan
iGEM 2012 Box 1 IGM021 J23151_GFP_pSB3K3: Bba_I20270 TOP10 Parts Kit Erik
iGEM 2012 Box 1 IGM022 J23101_GFP_pSB3K3: Bba_I20260 TOP10 Parts Kit Erik
iGEM 2012 Box 1 IGM023 pet21.KOD TOP10 Jared
iGEM 2012 Box 1 IGM024 pQL269 TOP10 Jared
iGEM 2012 Box 1 IGM025 pACYC.T7.GFP TOP10 Jared
iGEM 2012 Box 1 IGM026 pSB1A2-J06504 (RFP--incorrect sequence) TOP10 Parts Kit Ben
iGEM 2012 Box 1 IGM027 pAK.TAQ TOP10 Jared
iGEM 2012 Box 1 IGM028 PAAV-miniCMV-mCherry Bl-21 AI Addgene Logan
iGEM 2012 Box 1 IGM029 pET-SpA_ScTrp Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM030 pET-SpA_ScTrp_mCherry_5' Bl-21 AI Logan
iGEM 2012 Box 1 IGM031 pET-SpA_ScTrp_mCherry_5' Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM032 pET-SpA_ScTrp_mCherry_3' Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM033 pET-SpA_ScTrp Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM034 pAAV-miniCMV-mCherry Bl-21 DE3 Logan
iGEM 2012 Box 1 IGM035 psb3k3-J2315.ORF1 + pra301-ndmApro TOP10 Barrick Lab Peter
iGEM 2012 Box 1 IGM036 J23001-NdmABCDgst9-pSB1C3 BW25113-guaB Erik Erik
iGEM 2012 Box 1 IGM037 psb3k3-J2315.ORF1 TOP10 Barrick Lab Peter
iGEM 2012 Box 1 IGM038 none BW25113-guaB Erik Erik
iGEM 2012 Box 1 IGM039 pSB1C3-K537011 TOP10 iGEM parts kit Peter
iGEM 2012 Box 1 IGM040 pSB1C3-K537003 TOP10 iGEM parts kit Peter
iGEM 2012 Box 1 IGM041 NdmABCD BW25113-guaB Erik Razan
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Plasmids_and_Strains"