Team:Marburg SYNMIKRO/Primer

From 2012.igem.org

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==Primer==
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===Primer===
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!Primer!!Name!!Sequence!!Length!!G/C content!!TM
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!No.!!Name!!Sequence!!Length!!G/C content!!TM
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|<div id="MS1"></div>MS1||AmiC_ss_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'||xx bp||yy %||zz °C
+
|<div id="MS1"></div>MS1||AmiC_ss_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'||38 bp||55.2 %||86.5 °C
|-
|-
-
|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C
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|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||38 bp||63.1 %||85.5 °C
|-
|-
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|<div id="MS3"></div>MS3||MS3 HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA||16.07.12
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|<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'||37 bp||51.3 %||83.6 °C
|-
|-
-
|<div id="MS4"></div>MS4||MS4 HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC||16.07.12
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|<div id="MS4"></div>MS4||HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv||38 bp||57.8 %||82.8 °C
|-
|-
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|<div id="MS4b"></div>MS4b||MS4 (b) HU_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG||10.08.2012
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|<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'||45 bp||57.7 %||85.7 °C
|-
|-
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|<div id="MS5"></div>MS5||MS5 GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG||16.07.12
+
|<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'||120 bp||51.6 %||98.0 °C
|-
|-
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|<div id="MS6"></div>MS6||MS6 GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT||16.07.12
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|<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'||45 bp||57.7 %||88.7 °C
|-
|-
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|<div id="MS6b"></div>MS6b||MS6 (b) GroES_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA||10.08.2012
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|<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'||38 bp||52.6 %||81.4 °C
|-
|-
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|<div id="MS7"></div>MS7||MS7 GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA||16.07.12
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|<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'||54 bp||50.0 %||85.1 °C
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|-
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|<div id="MS8"></div>MS8||MS8 GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT||16.07.12
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|<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'||46 bp||50.0 %||82.7 °C
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|-
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|<div id="MS9"></div>MS9||MS9 Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC||16.07.12
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|<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'||42 bp||54.7 %||86.2 °C
|-
|-
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|<div id="MS10"></div>MS10||MS10 Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC||16.07.12
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|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'||41 bp||56.0 %||85.1 °C
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|-
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|<div id="MS11"></div>MS11||MS11 GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
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|<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||52 bp||50.0 %||87.6 °C
|-
|-
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|<div id="MS12"></div>MS12||MS12 RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
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|<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||72 bp||48.8 %||89.8 °C
|-
|-
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|<div id="MS13"></div>MS13||MS13 GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC||20.07.12
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|<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'||59 bp||45.7 %||85.5 °C
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|-
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|<div id="MS14"></div>MS14||MS14 RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT||20.07.12
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|<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'||41 bp||58.5 %||87.6 °C
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|-
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|<div id="MS15"></div>MS15||MS15 RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA||20.07.12
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|<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'||41 bp||56.0 %||84.2 °C
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|-
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|<div id="MS16"></div>MS16||MS16 CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT||20.07.12
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|<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'||43 bp||62.7 %||90.3 °C
|-
|-
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|<div id="MS17"></div>MS17||MS17 CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT||20.07.12
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|<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'||43 bp||51.1 %||80.5 °C
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|-
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|<div id="MS18"></div>MS18||MS 18 Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG||10.08.2012
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|<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'||28 bp||53.5 %||77.0 °C
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|-
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|<div id="MS19"></div>MS19||MS 19 Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA||10.08.2012
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|<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'||29 bp||55.1 %||75.3 °C
|-
|-
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|<div id="MS20"></div>MS20||MS 20 VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA||10.08.2012
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|<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA-3'||20 bp||50.0 %||63.5 °C
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|-
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|<div id="MS21"></div>MS21||MS 21 VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC||10.08.2012
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|<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC-3'||20 bp||50 %||63.1 °C
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|<div id="MS22"></div>MS22||MS22 SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA||20.08.2012
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|<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA-3'||22 bp||59.0 %||77.2 °C
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|-
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|<div id="MS23"></div>MS23||MS23 SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG||20.08.2012
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|<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG-3'||26 bp||38.4 %||67.6 °C
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|-
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|<div id="MS24"></div>MS24||MS24 Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
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|<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG-3'||20 bp||65.0 %||68.8 °C
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|-
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|<div id="MS25"></div>MS25||MS25 Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
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|<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC-3'||21 bp||57.1 %||72.2 °C
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|<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
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|<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'||42 bp||47.6 %||84.8 °C
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|-
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|<div id="MS27"></div>MS27||SacB_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
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|<div id="MS27"></div>MS27||SacB_std_10_rev||5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'||40 bp||45.0 %||76.8 °C
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{{:Team:Marburg_SYNMIKRO:Template:Footer}}
{{:Team:Marburg_SYNMIKRO:Template:Footer}}

Latest revision as of 23:33, 26 September 2012

Main Page

Primer

No.NameSequenceLengthG/C contentTM
MS1		AmiC_ss_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'38 bp55.2 %86.5 °C
MS2		AmiC_ss_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'38 bp63.1 %85.5 °C
MS3		HU_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'37 bp51.3 %83.6 °C
MS4		HU_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv38 bp57.8 %82.8 °C
MS4b		HU_std_10_rev25'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'45 bp57.7 %85.7 °C
MS5		GroEs_std_10_fwd5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA
CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'		120 bp51.6 %98.0 °C
MS6		GroEs_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'45 bp57.7 %88.7 °C
MS6b		GroES_std_10_rev25'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'38 bp52.6 %81.4 °C
MS7		GixR_std_10_fwd5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'54 bp50.0 %85.1 °C
MS8		GixR_std_10_rev5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'46 bp50.0 %82.7 °C
MS9		Enhancer_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'42 bp54.7 %86.2 °C
MS10		Enhancer_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'41 bp56.0 %85.1 °C
MS11		GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'52 bp50.0 %87.6 °C
MS12		RBS + GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC
TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'		72 bp48.8 %89.8 °C
MS13		GIN_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'59 bp45.7 %85.5 °C
MS14		RFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'41 bp58.5 %87.6 °C
MS15		RFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'41 bp56.0 %84.2 °C
MS16		CFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'43 bp62.7 %90.3 °C
MS17		CFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'43 bp51.1 %80.5 °C
MS18		Prefix_std_10_fwd5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'28 bp53.5 %77.0 °C
MS19		Suffix_std_10_rev5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'29 bp55.1 %75.3 °C
MS20		VF2_std_10_fwd5'-TGCCACCTGACGTCTAAGAA-3'20 bp50.0 %63.5 °C
MS21		VR_std_10_rev5'-ATTACCGCCTTTGAGTGAGC-3'20 bp50 %63.1 °C
MS22		SB-prep-3P-15'-GCCGCTGCAGTCCGGCAAAAAA-3'22 bp59.0 %77.2 °C
MS23		SB-prep-2Ea5'-ATGAATTCCAGAAATCATCCTTAGCG-3'26 bp38.4 %67.6 °C
MS24		Suffix_std_10_fwd5'-ACTAGTAGCGGCCGCTGCAG-3'20 bp65.0 %68.8 °C
MS25		Prefix_std_10_rev5'-TCTAGATGCGGCCGCGAATTC-3'21 bp57.1 %72.2 °C
MS26		SacB_std_10_fwd5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'42 bp47.6 %84.8 °C
MS27		SacB_std_10_rev5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'40 bp45.0 %76.8 °C

Retrieved from "http://2012.igem.org/Team:Marburg_SYNMIKRO/Primer"