Team:TU-Eindhoven/LEC/Accomplishments

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[[File:Gold medal.png|50px|left]]  
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<table border='0' width='710' cellpadding='10'>
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<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Register the team, have a great summer and plan to have fun at the Regional Jamboree</h3>
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As can also be seen at our [[Team:TU-Eindhoven/Photos|photos]] we had a <span class="lightblue">great summer</span>!</td></tr>
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<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Complete the judging form</h3>
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This was completed and submitted.</td></tr>
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[[File:Silver medal-blue.png|50px|left]]  
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<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Create and present a wiki, poster and talk</h3>
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As you can see our wiki is done and of course we are going to present our poster and talk at the <span class="lightblue">Regional Jamboree</span>.</td></tr>
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[[File:Bronze medal.png|50px|left]]
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<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Create and document a new BioBrick<sup>TM</sup></h3>
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<span class="lightblue">Created</span> new Ca<sup>2+</sup> sensor BioBricks<sup>TM</sup>. See [[Team:TU-Eindhoven/LEC/BioBricks|our BioBricks<sup>TM</sup>]].</td></tr>
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<tr valign="top"><td>[[File:Silver medal-blue.png|50px|left|link=]] </td><td>
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<h3>Our new BioBrick<sup>TM</sup> has been demonstrated and characterized</h3>
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We have created BioBricks<sup>TM</sup> from the GECO proteins, these are demonstrated and characterized as shown [[Team:TU-Eindhoven/LEC/BioBricks|here]]. This information has been entered in the registry.</td></tr>
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<tr valign="top"><td>[[File:Gold medal.png|50px|left|link=]]</td><td>
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<h3>Discovery Festival</h3>
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We organized an iGEM workshop on the Discovery Festival in the Netherlands in collaboration with 3 other Dutch iGEM teams: <span class="lightblue">Amsterdam, Groningen and Wageningen_UR</span>. To cooperate in this large project, the 4 teams met a couple of times on different locations in the Netherlands. Besides these meetings, we discussed a lot during several Skype-meetings. We will take our visitors on a tour in our provisional laboratory, where they will learn about the process of genetic modification. The festival will take place in 3 cities, Amsterdam, Rotterdam and Eindhoven, and over 5000 visitors are expected. Hopefully, this will <span class="lightblue">inspire</span> other teams in upcoming years to combine forces in reaching out to the public. More information can be found on [[Team:TU-Eindhoven/Human_Practices|the public outreach page]].</td></tr>
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<tr valign="top"><td>[[File:Gold medal.png|50px|left|link=]]</td><td>
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<h3>Collaborations</h3>
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We met all Dutch iGEM teams during a <span class="lightblue">symposium</span> in the first week of the summer break. Furthermore, we posted <span class="lightblue">two offers on the NTNU-Trondheim Matchmaker</span>, we helped the teams of Queens_Canada and Trieste with their computational models and we came up with 'tips and tricks' for new iGEM teams for the team of Cinvestav. Just before the Regional Jamboree, the Dutch teams will meet again during a <span class="lightblue">symposium in Eindhoven</span>, where every team can rehearse their final presentation. Some German and Belgian teams were also invited to this symposium. See our <html><a href="https://2012.igem.org/Team:TU-Eindhoven/Collaborations">collaborations page</a></html>.</td></tr>
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<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
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<h3>Device</h3>
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We made a working <html><a href="https://2012.igem.org/Team:TU-Eindhoven/LEC/Device">device</a></html> and created elaborate software for it.</td></tr>
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<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
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<h3>Model</h3>
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A sensitivity analysis is performed to a <html><a href="https://2012.igem.org/Team:TU-Eindhoven/LEC/ModellingFirst">basic calcium model</a></html>, first used by the iGEM team of Valencia in 2009, so we found out which <span class="lightblue">parameters</span> are <span class="lightblue">most sensitive</span>. Furthermore, we made an <html><a href="https://2012.igem.org/Team:TU-Eindhoven/Modeling">extended calcium model</a></html>, in which the kinetics of the calcium homeostasis, voltage-gated calcium channels and GECO proteins are taken into account. By combining these three characteristics, we created a model for a <span class="lightblue">completely new system</span>.</td></tr>
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<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
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<h3>Our yeast cells</h3>
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In the lab, we <span class="lightblue">created</span> three kinds of <html><a href=" https://2012.igem.org/Team:TU-Eindhoven/LEC/Lab">yeast cells</a></html>, containing both the channel proteins CCH1 and MID1 and a red, green or blue GECO calcium sensor.</td></tr>
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<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
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<h3>Challenge in working with yeast cells</h3>
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By choosing a yeast cell as the basis for our project, we created a challenge for ourselves. Most iGEM work is done with E. coli and relatively few projects focus on yeast. Therefore less knowledge is available and <span class="lightblue">few compatible BioBricks<sup>TM</sup></span> have been developed. On the other hand, it creates a greater opportunity for us to <span class="lightblue">contribute to the Registry</span>. Also, our project is focused on a system in which a respond to <span class="lightblue">exogenous stimuli</span> is important, whereas many previous iGEM projects focus on intracellular processes. Again, we hope we created new knowledge which is usable for upcoming teams.
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Latest revision as of 02:19, 27 September 2012

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