Team:Wageningen UR/FinalOverview

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Template:WUR}} = Final Overview == <p align="justify"> Concluding this iGEM project, we are looking back on a great team effort with very steep individual learning curves. W...")
 
(62 intermediate revisions not shown)
Line 1: Line 1:
{{Template:WUR}}
{{Template:WUR}}
-
= Final Overview ==
+
<html><script>document.getElementById("header_slider").style.backgroundImage = "url(https://static.igem.org/mediawiki/2012/6/60/HeaderProject.jpg)";</script></html>
 +
= Final Overview =
 +
 +
[[File:VLPoverview1.jpg|right|center|950px]]
<p align="justify">
<p align="justify">
-
Concluding this iGEM project, we are looking back on a great team effort with very steep individual learning curves. We started with the dream of making the next step in site-specific drug delivery.
 
-
"If it turns out that the combination of delivery and medicine has a superior ability to cure, everything is achievable in the modern world." Senior Project Leader in veterinary medicine at MSD[].
+
Concluding this iGEM project, we are looking back on a great team effort with very steep individual learning curves. We started with the dream of making the next step in [[Team:Wageningen_UR/Applications#Site-specific_drug_delivery|site-specific drug delivery]].
-
Virus like particles could facilitate this next step. But, as the project leader indicates, íf it turns out… So, how far did we actually get?  
+
<br>
 +
</p>
 +
<p align="right">'''"If it turns out that the combination of delivery and medicine has a superior ability to cure, everything is achievable in the modern world."'''
 +
<br>
 +
Senior Project Leader in veterinary medicine at [http://www.merck.com/index.html MSD].
 +
</p>
 +
<p align="justify">
 +
<br>
 +
Virus-like particles could facilitate this next step. But, as the project leader indicates, íf it turns out… So, how far did we actually get?  
 +
<br>
 +
<br>
-
Starting with only the basic templates of the VLP genes [link to each virus], or even just some infected leafs [link polero], we managed to produce[], assemble[], purify[], detect [dls], visualize [em] and modify [neg inside] Virus-Like Particles.  
+
Starting with only the basic templates of the [[Team:Wageningen_UR/VLPs|VLP genes]], or even just some infected [[Team:Wageningen_UR/ObtainingthePoleroVLP|leafs]], we managed to [[Team:Wageningen_UR/Protocol/StartupCCMV|produce]], [[Team:Wageningen_UR/Protocol/DialysisCCMV|assemble]], [[Team:Wageningen_UR/Protocol/RoundupCCMV|purify]], [[Team:Wageningen_UR/MethodsDetection#Dynamic_Light_Scattering_.28DLS.29|detect]], [[Team:Wageningen_UR/MethodsDetection#Electron_Microscopy_.28EM.29|visualize]] and [[Team:Wageningen_UR/InsideModification#CCMV_with_a_negative_interior|modify]] Virus-Like Particles.  
-
Up to this point, we did not manage to make a modification to the outside [] of a VLP, nor did we manage to see the PnA system[] work as intended. However, we did manage to design a multi-step pcr reaction[] which can attach either the k- or e-coil to either termini of any protein. Due to an unintended frameshift[], we were not able to produce a VLP with the coil on the inside [].  
+
Up to this point, we did not manage to make a modification to the [[Team:Wageningen_UR/OutsideModification|outside]] of a VLP, nor did we manage to see the [[Team:Wageningen_UR/Coil_system|PnA system]] work as intended. However, we did manage to design a [[Team:Wageningen_UR/InsideModification#Methods|multi-step PCR reaction]] which can attach either the [[Team:Wageningen_UR/Coil_system#E-_and_K-coil|K- or E-coil]] to either termini of any protein. Due to an unintended [[Team:Wageningen_UR/InsideModification#Results|frameshift]], we were not able to produce a VLP with the coil on the [[Team:Wageningen_UR/InsideModification|inside]]. However, we fixed the frameshift in the dying days of the project, but did not get to the point of expression.  
-
To help other teams in preventing mistakes, we co-developed and published[] The Constructor[], a web-based cloning tool that finds the fastest way to assemble BioBrick Devices. The Constructor always[link to table results>>constructor] gives an equal or better result compared to manual deduction of a cloning strategy.
+
To help other teams in preventing mistakes, we co-developed and [[Team:Wageningen_UR/TheConstructor#Our_Publication|published]] [[Team:Wageningen_UR/TheConstructor|The Constructor]], a web-based cloning tool that finds the fastest way to assemble BioBrick Devices. The Constructor's results are more easily acquired and equal or faster [[Team:Wageningen_UR/TheConstructor#Results|compared]] to manual deduction of a cloning strategy.
-
The functionality of The Constructor depends on the quality of the BioBricks in the Parts Registry. During the project, we encountered multiple parts that were faulty[]. We sequenced and analysed these parts and reported back to the registry. Besides, we added a functionality to GFP[] and standardized the KILR coil[].  
+
The functionality of The Constructor depends on the quality of the BioBricks in the Parts Registry. During the project, we encountered multiple parts that were [[Team:Wageningen_UR/Parts#Faulty_parts|faulty]]. We sequenced and analysed these parts and reported back to the registry. Besides, we added functionality to [https://2012.igem.org/Team:Wageningen_UR/Coil_system#GFP_with_the_E-coil GFP] of which the expression has been [[Team:Wageningen_UR/Coil_system#GFP_with_E-coil|checked]] and standardized the [[Team:Wageningen_UR/Parts#Parts_we_standardized|KILR coil]].  
-
Presenting [Munich] and communicating [Communication] our ideas outside the confined area of the lab both gave us a more realistic view on the perception of synthetic biology by stakeholders[st], the public[Discovery], school kids[sec school] and fellow iGEMmers[mini sym] and gave us the opportunity to spread our ideas and the vision of the iGEM competition.
 
-
This gave us also great feedback on other applications[] for modified VLPs. Because it does not stop at site-specific drug delivery. One could imagine a standardized vaccine carrier[], or a nano-bio-reactor[].
+
[[Team:Wageningen_UR/CASconference|Presenting]] at [http://www.rug.nl/fmns-research/csb/euroSynbioProgrammeGroningenSept.pdf two] [http://www.cas.uni-muenchen.de/veranstaltungen/tag_synth_bio_2012/program.pdf international conferences] and [[Team:Wageningen_UR/Human_Practices#Human_practices|communicating]] our ideas outside the confined area of the lab both gave us a more realistic view on the perception of synthetic biology by [[Team:Wageningen_UR/Stakeholders|stakeholders]], the [[Team:Wageningen_UR/DiscoveryFestival|public]], [[Team:Wageningen_UR/Visiting_Secondary_School|school kids]] and fellow [[Team:Wageningen_UR/miniSymposium|iGEMmers]] and gave us the opportunity to spread our ideas and the vision of the iGEM competition. The stakeholders gave us the indication that a device like ours is needed, so we checked the applicability with a [[Team:Wageningen_UR/HumanBody|human body model]]. The [[Team:Wageningen_UR/HumanBody#Results|outcomes]] were promising.  
-
“Having in place a system that can rapidly develop a vaccine against unexpected viral agents would be of great importance for public health.” ECDC Program Leader Vaccine Preventable Diseases
+
Talking with all these people gave us new insight on other [[Team:Wageningen_UR/Applications|applications]] for modified VLPs: It does not stop at site-specific drug delivery. One could imagine a [[Team:Wageningen_UR/Applications#Standardized_vaccine_platforms|standardized vaccine carrier]], or a [[Team:Wageningen_UR/Applications#Nanomaterials.2C_nanoreactors_and_more|nano-bio-reactor]].
-
For all the possible applications, We made a major first step by standardizing the Virus-Like Particles. So, we will continue our efforts to realise the dream.
+
<br>
</p>
</p>
 +
<p align="right">
 +
'''“Having in place a system that can rapidly develop a vaccine against unexpected viral agents would be of great importance for public health.”'''
 +
<br>
 +
ECDC Program Leader Vaccine Preventable Diseases
 +
</p>
 +
<p align="justify">
 +
<br>
 +
For all the possible applications, we made a major first step by standardizing Virus-Like Particles in BioBrick format. We hope others will continue our efforts to realize the dream of using VLPs for site-specific drug delivery or explore further applications.
 +
[[File:VLPoverview2.jpg|center|950px]]
 +
 +
</p>
 +
 +
<html>
 +
<div class="prNav">
 +
<div class="prev">
 +
  <a href="https://2012.igem.org/Team:Wageningen_UR/TheConstructor" title="The Constructor">8. The Constructor</a>
 +
</div>
 +
</div>
 +
</html>

Latest revision as of 19:19, 26 October 2012

Final Overview

VLPoverview1.jpg

Concluding this iGEM project, we are looking back on a great team effort with very steep individual learning curves. We started with the dream of making the next step in site-specific drug delivery.

"If it turns out that the combination of delivery and medicine has a superior ability to cure, everything is achievable in the modern world."
Senior Project Leader in veterinary medicine at [http://www.merck.com/index.html MSD].


Virus-like particles could facilitate this next step. But, as the project leader indicates, íf it turns out… So, how far did we actually get?

Starting with only the basic templates of the VLP genes, or even just some infected leafs, we managed to produce, assemble, purify, detect, visualize and modify Virus-Like Particles. Up to this point, we did not manage to make a modification to the outside of a VLP, nor did we manage to see the PnA system work as intended. However, we did manage to design a multi-step PCR reaction which can attach either the K- or E-coil to either termini of any protein. Due to an unintended frameshift, we were not able to produce a VLP with the coil on the inside. However, we fixed the frameshift in the dying days of the project, but did not get to the point of expression. To help other teams in preventing mistakes, we co-developed and published The Constructor, a web-based cloning tool that finds the fastest way to assemble BioBrick Devices. The Constructor's results are more easily acquired and equal or faster compared to manual deduction of a cloning strategy. The functionality of The Constructor depends on the quality of the BioBricks in the Parts Registry. During the project, we encountered multiple parts that were faulty. We sequenced and analysed these parts and reported back to the registry. Besides, we added functionality to GFP of which the expression has been checked and standardized the KILR coil. Presenting at [http://www.rug.nl/fmns-research/csb/euroSynbioProgrammeGroningenSept.pdf two] [http://www.cas.uni-muenchen.de/veranstaltungen/tag_synth_bio_2012/program.pdf international conferences] and communicating our ideas outside the confined area of the lab both gave us a more realistic view on the perception of synthetic biology by stakeholders, the public, school kids and fellow iGEMmers and gave us the opportunity to spread our ideas and the vision of the iGEM competition. The stakeholders gave us the indication that a device like ours is needed, so we checked the applicability with a human body model. The outcomes were promising. Talking with all these people gave us new insight on other applications for modified VLPs: It does not stop at site-specific drug delivery. One could imagine a standardized vaccine carrier, or a nano-bio-reactor.

“Having in place a system that can rapidly develop a vaccine against unexpected viral agents would be of great importance for public health.”
ECDC Program Leader Vaccine Preventable Diseases


For all the possible applications, we made a major first step by standardizing Virus-Like Particles in BioBrick format. We hope others will continue our efforts to realize the dream of using VLPs for site-specific drug delivery or explore further applications.

VLPoverview2.jpg

Retrieved from "http://2012.igem.org/Team:Wageningen_UR/FinalOverview"