Team:Ehime-Japan/HumanPractice-E.create

From 2012.igem.org

(Difference between revisions)
 
(24 intermediate revisions not shown)
Line 1: Line 1:
 +
<html>
 +
<style type="text/css">
 +
 +
body {
 +
background-color: #0077ff;
 +
color: #0077ff;
 +
margin: auto;
 +
padding: 0;
 +
 +
}
 +
 +
 +
</style>
 +
 +
<head>
 +
<title>Ehime-Japan iGEM Team: Welcome</title>
 +
<script src="http://cdn.jquerytools.org/1.2.5/full/jquery.tools.min.js?foo"></script>
 +
<style type="text/css">
 +
/*hide default igem banner and reformat style into blank slate*/
 +
#globalWrapper {width: 100%;}
 +
#top-section {width: 100%; height:30px; border:none;}
 +
#p-logo {display:none;}
 +
#search-controls {display:none;}
 +
#menubar a {color:#000000;}
 +
#menubar a:hover{text-decoration:none; color:#ff0000;}
 +
.left-menu {background-color:#FFFFFF; margin:5px 0px 0px 0px; padding:0;}
 +
.left-menu ul {background-color:#FFFFFF; margin:0; padding:0;}
 +
.right-menu ul li a {background-color:#FFFFFF;}
 +
.printfooter {display:none;}
 +
#footer-box {border:none;}
 +
#catlinks {display:none;}
 +
.firstHeading {display:none;}
 +
#content {width: 100%; border:none;}
 +
#bodyContent {border:none;}
 +
 +
/*actual content styles*/
 +
body {width: 800px; margin:auto;}
 +
 +
#Ehime-Japan_wiki_content {height:auto; line-height:100%;}
 +
/*#Ehime-Japan_wiki_content a {color:#69d01d;}*/
 +
#Ehime-Japan_wiki_content a:hover {text-decoration:none; color:#3d3f3c;}
 +
 +
.navbar li {color: #ffffff;}
 +
.navbar li a {color: #ffffff;}
 +
.navbar li a:hover {background:#69d01d; color: #ffffff;}
 +
 +
/*only use for current page content header (i.e. Team, G-nomeSurferPro, etc)*/
 +
H6 {
 +
      font-family: Helvetica;
 +
      text-transform: uppercase;
 +
      text-decoration: none;
 +
      text-align: center;
 +
      color: #B22222;
 +
      font-size: 32pt;
 +
    }
 +
 +
H3 {
 +
      font-family: Helvetica;
 +
      text-decoration: none;
 +
      text-align: left;
 +
          line-height:120%;
 +
      color: #958E8E;
 +
      font-size: 12pt;
 +
    }
 +
 +
</style>
 +
 +
<link rel="stylesheet" type="text/css" href="http://cs.wellesley.edu/~hcilab/iGEM_wiki/css/Team.css">
 +
 +
 +
 +
 +
 +
</head>
 +
 +
<body>
 +
<body class="basiclayout">
 +
<div id="Ehime-Japan_wiki_content">
 +
 +
<p  style="text-align:center;"><a href="https://2012.igem.org/Team:Ehime-Japan"><img src="https://static.igem.org/mediawiki/2012/b/b4/Ecolingual%E3%82%A6%E3%82%A3%E3%82%AD%E7%94%A8%EF%BC%92.png" width="800px"></a></p>
 +
<br>
 +
 +
<ul id="nav">
 +
        <li><a href="https://2012.igem.org/Team:Ehime-Japan">Home</a></li> 
 +
<li><a href="https://2012.igem.org/Team:Ehime-Japan/Project">Project</a>
 +
<ul>
 +
<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#one">E.co-mail</a></li>
 +
<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#two">E.co-Domino</a></li>
 +
                        <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#three">E.cold-heat sensing system</a></li>
 +
<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#four">E.colingual</a></li>
 +
</ul>
 +
</li>
 +
        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Modeling">Modeling</a></li>
 +
<li><a href="#">Data</a>
 +
<ul>
 +
<li><a href="https://2012.igem.org/Team:Ehime-Japan/Parts">BioBrick parts</a></li>
 +
<li><a href="https://2012.igem.org/Team:Ehime-Japan/PlasmidsAndStrain">Plasmids And Strain</a></li>
 +
<li><a href="https://2012.igem.org/Team:Ehime-Japan/PrimerList">Primer List</a></li>
 +
 +
</ul>
 +
</li>
 +
<li><a href="#">Human Practice</a>
 +
<ul>
 +
<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create">E.create</a></li>
 +
<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire">Questionnaire</a></li>
 +
 +
</ul>
 +
</li>
 +
 +
<li><a href="#">Other</a>
 +
<ul>
 +
<li><a href="https://2012.igem.org/Team:Ehime-Japan/Team">team</a></li>
 +
<li><a href="https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan">team profile</a></li>
 +
                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Notebook">note book</a></li>
 +
                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Safety">safety</a></li>
 +
                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Attributions">attribution</a></li> 
 +
</ul>
 +
</li>
 +
</ul>
 +
 +
<br>
<br>
<br>
<br>
Line 4: Line 125:
<font color=orange>
<font color=orange>
・E.create!
・E.create!
 +
<br>
<br>
<br>
<br>
<br>
Line 23: Line 145:
</p>
</p>
<font color=blue>
<font color=blue>
 +
<p>
・Promoter,
・Promoter,
<br>
<br>
Line 34: Line 157:
<br>
<br>
・Drug registance genes (KmR, CmR and AmpR).
・Drug registance genes (KmR, CmR and AmpR).
-
 
+
</p>
<br>
<br>
<br>
<br>
-
<font color=skyblue>
+
<font color=green>
☆ How to play  
☆ How to play  
Line 48: Line 171:
<br>
<br>
Ex. This ''E.coli'' can express GFP constitutively and has Km resistance.
Ex. This ''E.coli'' can express GFP constitutively and has Km resistance.
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/1/1b/%E3%81%A9%E3%82%84%EF%BC%92.png"
 +
width="250px">  E.colevel.0 Doya
 +
<br>
 +
<p>
 +
・He always makes GFP.
 +
<br>
 +
・He has Km resistance.
 +
</p>
 +
<br>
 +
<br>
 +
<p>
 +
You can choose the proper cards from the bottom and try to build the plasmid expressing GFP in E.coli!
 +
<br>
 +
※Turn the cards you do not use face-down and put a card of resistances on the center.
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2012/5/5f/P1000479.JPG"width="350px">
 +
<br>
 +
<br>
 +
Finish !
 +
<br>
 +
<p>
 +
One of the answers is this !
 +
<br>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/2012/8/83/P1000480.JPG"width="350px">
 +
<br>
 +
<p>
 +
Did you enjoy it? If you play more, let's challenge to make the E.colis !
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/b/b9/%E7%99%92%E3%81%97%E7%B3%BB%E5%A4%A7%E8%85%B8%E8%8F%8C.png"width="200px">  E.colevel.1 Ao
 +
<p>
 +
・He always makes CFP.
 +
<br>
 +
・He has Cm resistance.
 +
</p>
-
<img src="https://2012.igem.org/File:%E3%81%A9%E3%82%84%EF%BC%92.png"
 
-
width="500px">
 
-
<font size="5"><font color=green><br>
 
<br>
<br>
-
・Questionnaire
+
<img src="https://static.igem.org/mediawiki/2012/b/b8/%E3%82%AD%E3%83%A5%E3%83%BC%E3%83%88%E3%81%AA%E5%A4%A7%E8%85%B8%E8%8F%8C.png"width="200px"> E.colevel.2 Hana chan
-
<font size="3"><font color=black><br>
+
<p>
<p>
-
Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together!
+
・She always makes toxic to E.coli.
-
We obtained a lot of answers from people in different regions.
+
<br>
 +
・She makes RFP when toxic is produced.
 +
<br>
 +
・She has Amp resistance.
</p>
</p>
 +
 +
         
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/7/7f/%E9%83%B5%E4%BE%BF%E5%B1%8B%E3%81%95%E3%82%93%E5%A4%A7%E8%85%B8%E8%8F%8C.png"width="200px">  E.colevel.3 Postman X
 +
<br>
 +
<br>
 +
<p>
 +
In addition, we did presentation about sythetic biology for high school students and enjoyed playing them with
 +
E.create on the Open Campus days of University (August 7 and 8) !
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/1/10/%E6%B1%BA%E5%AE%9A_%284%29.jpg"width="350px">
 +
<img src="https://static.igem.org/mediawiki/2012/1/14/%E6%B1%BA%E5%AE%9A_%283%29.jpg"width="350px">
 +
<br>
 +
<br>
 +
 +
<p>
 +
On the other day, a few members of our team visited a high school to do educational activity and play E.create together. We taught about 50 students who are learning biology. Thank to everyone!
 +
</p>
 +
<br>
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/6/6d/P1000306.JPG"width="350px">
 +
<img src="https://static.igem.org/mediawiki/2012/b/b3/P1000318.JPG"width="350px">
 +
<br>
 +
<br>
 +
<br>
 +
 +
<p><font size="5"><font color=orange>
 +
Evaluation of our activities above.</font size></font color><p>
 +
<p><font size="3">
 +
<p>
 +
We asked the participants in our educational activities the following questions.
 +
</p>
 +
<br>
 +
Questions about gene recombination__
 +
<br>
 +
<p>
 +
1.Before our lecture and playing E.create
 +
<br>
 +
Q1. Do you know gene recombination?
 +
<br>
 +
Q2. How do you feel toward gene recombination?
 +
<br>
 +
</p>
 +
<br>
 +
<br>
 +
<p>
 +
2. After our lecture and playing E.create
 +
<br>
 +
Q3. Did you change your idea to Q1?
 +
<br>
 +
Q4. Did you change your idea to Q2?
 +
<br>
 +
Q5. Did you change your interest in gene recombination by comparison with before the lecture and playing E.create?
 +
</p>
 +
<br>
 +
<br>
 +
Answers to the questions
 +
Answers to Q1 and Q3, Q2 and Q4, and Q5 are shown below.
 +
<br>
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/e/e6/P1000560.JPG"width="350px">
 +
<img src="https://static.igem.org/mediawiki/2012/3/38/%E2%91%A1.png"width="350px">
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/4/49/%E2%91%A2.png"width="350px">
 +
<img src="https://static.igem.org/mediawiki/2012/c/c3/%E2%91%A3.png"width="350px">
 +
<br>
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2012/a/a8/%EF%BC%96.png"width="500px">
 +
<br>
 +
<br>
 +
<p>
 +
The answers toward those questions told us that our educational activities were greatly successful !!!
 +
<br>
 +
<br>
 +
<br>
 +
Also, we have a plan to distribute E.create around Japan in order to help studying synthetic biology.
 +
In Japan, the standards for the contents of high-school biology courses are changing.
 +
From the next year, high school students will study more about molecular biology and genetic engineering
 +
than before. If the students read the text only, it is difficult to imagine how genetic engineering
 +
works. So we hope that E.create will help them understand it more effectively and deeply.
 +
</p>
 +
<br>
 +
<br>
<br>
<br>

Latest revision as of 01:27, 27 September 2012

Ehime-Japan iGEM Team: Welcome




・E.create!


Our motivation

We made a BioBrick card game “E.create” to introduce to a number of people the enjoyability of synthetic biology. Nobody in our team had learned synthetic biology until we started our iGEM project. We thought from our experiences that the construction of the plasmids is the most interesting process. We hope that everybody who is interested in life sciences and genetic engineering can enjoy learning synthetic biology by playing with E.create!


E.create is a puzzle game. The kit contains cards with illustrations on them. Each card corresponds to one of the gene parts including:

・Promoter,
・RBS (Ribosome Binding Site),
・Protein coding regions,
・Terminator,
・Ori (Origin), and
・Drug registance genes (KmR, CmR and AmpR).



☆ How to play

Players will challenge to constract one or more plasmid(s) that should be required to transform ''E.coli'' to give properties presented by the puzzle master. An example is shown below. Let’s make it!


Ex. This ''E.coli'' can express GFP constitutively and has Km resistance.

E.colevel.0 Doya

・He always makes GFP.
・He has Km resistance.



You can choose the proper cards from the bottom and try to build the plasmid expressing GFP in E.coli!
※Turn the cards you do not use face-down and put a card of resistances on the center.



Finish !

One of the answers is this !


Did you enjoy it? If you play more, let's challenge to make the E.colis !

E.colevel.1 Ao

・He always makes CFP.
・He has Cm resistance.


E.colevel.2 Hana chan

・She always makes toxic to E.coli.
・She makes RFP when toxic is produced.
・She has Amp resistance.



E.colevel.3 Postman X

In addition, we did presentation about sythetic biology for high school students and enjoyed playing them with E.create on the Open Campus days of University (August 7 and 8) !



On the other day, a few members of our team visited a high school to do educational activity and play E.create together. We taught about 50 students who are learning biology. Thank to everyone!







Evaluation of our activities above.

We asked the participants in our educational activities the following questions.


Questions about gene recombination__

1.Before our lecture and playing E.create
Q1. Do you know gene recombination?
Q2. How do you feel toward gene recombination?



2. After our lecture and playing E.create
Q3. Did you change your idea to Q1?
Q4. Did you change your idea to Q2?
Q5. Did you change your interest in gene recombination by comparison with before the lecture and playing E.create?



Answers to the questions Answers to Q1 and Q3, Q2 and Q4, and Q5 are shown below.









The answers toward those questions told us that our educational activities were greatly successful !!!


Also, we have a plan to distribute E.create around Japan in order to help studying synthetic biology. In Japan, the standards for the contents of high-school biology courses are changing. From the next year, high school students will study more about molecular biology and genetic engineering than before. If the students read the text only, it is difficult to imagine how genetic engineering works. So we hope that E.create will help them understand it more effectively and deeply.




Retrieved from "http://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create"