Team:Cornell/testing/protocols

From 2012.igem.org

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<ul>
<ul>
<li>
<li>
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<a href="#">Miniprep</a>
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<a href="https://static.igem.org/mediawiki/2012/4/46/EZNA_Gel_Extraction.pdf" target="_blank">EZNA Gel Extraction</a>
</li>
</li>
<li>
<li>
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<a href="#">Gel Extraction</a>
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<a href="https://static.igem.org/mediawiki/2012/3/35/Ezna_Miniprep.pdf" target="_blank">EZNA Miniprep</a>
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</li>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/2/21/Qiagen_Gel_Extraction.pdf" target="_blank">Qiagen Gel Extraction</a>
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</li>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
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</li>
</ul>
</ul>
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<ul>
<ul>
<li>
<li>
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<a href="#">Gel Electrophoresis</a>
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<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
</li>
</li>
<li>
<li>
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<a href="#">DNA Quantification</a>
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<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
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<a href="#">Sequencing</a>
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<a href="#">PCR Clean Up</a>
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</li>
</li>
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</ul>
</ul>
</div>
</div>
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<ul>
<ul>
<li>
<li>
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<a href="#">Vent PCR</a>
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<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
</li>
</li>
<li>
<li>
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<a href="#">Phusion PCR</a>
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<a href="https://static.igem.org/mediawiki/2012/4/4b/Taq_PCR.pdf" target="_blank">Taq PCR</a>
</li>
</li>
<li>
<li>
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<a href="#">Colony PCR</a>
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<a href="https://static.igem.org/mediawiki/2012/3/33/Vent_PCR.pdf" target="_blank">Vent PCR</a>
</li>
</li>
</ul>
</ul>
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<ul>
<ul>
<li>
<li>
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<a href="#">Transformation of E. coli</a>
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<a href="https://static.igem.org/mediawiki/2012/0/07/Ecoli_electroporation.pdf" target="_blank">Electroporation of <i>E.coli</i></a>
</li>
</li>
<li>
<li>
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<a href="#">Transformation of Shewanella</a>
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<a href="https://static.igem.org/mediawiki/2012/5/57/Making_Ecoli_Electrocompetent.pdf" target="_blank">Preparing Electrocompetent <i>E.coli</i></a>
</li>
</li>
<li>
<li>
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<a href="#">Conjugation</a>
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<a href="https://static.igem.org/mediawiki/2012/6/6f/MyersMyers_Shew-E-poration.pdf" target="_blank">Myers and Myers Electoporation of <i>Shewanella</i></a>
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</li>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/f/f7/PNNL_Electrocompetent_Shewanella.pdf" target="_blank">PNNL’s Preparing Electrocompetent <i>Shewanella</i></a>
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</li>
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<li>
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<a href="https://static.igem.org/mediawiki/2012/0/0f/Shewanella_Conjugation.pdf" target="_blank">Conjugation of Plasmids into <i>Shewanella</i> </a>
</li>
</li>
</ul>
</ul>
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<ul>
<li>
<li>
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<a href="#">Digestion Reaction</a>
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<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
</li>
</li>
<li>
<li>
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<a href="#">Dephosphorylation</a>
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<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
</li>
</li>
<li>
<li>
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<a href="#">Ligation Reaction</a>
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<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
</li>
</li>
<li>
<li>
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<a href="#">Gibson Assembly</a>
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<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
</li>
</li>
</ul>
</ul>
</div>
</div>
<div class="four columns">
<div class="four columns">
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<h4>Maintenance Tasks</h4>
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<h4><i>Shewanella</i> Growth</h4>
<ul>
<ul>
<li>
<li>
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<a href="#">Making LB</a>
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<a href="https://static.igem.org/mediawiki/2012/6/6c/Growth_Assay_Protocol.pdf" target="_blank"><i>Shewanella</i> Growth Assay Protocol</a>
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</li>
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<li>
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<a href="#">Making Super Optimal Broths</a>
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<a href="#">Making Plates</a>
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</li>
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<a href="#">Autoclave</a>
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<li>
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<a href="#">Preparing Glycerol Stocks</a>
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</li>
</li>
<li>
<li>
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<a href="#">Preparing Overnight Cultures</a>
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<a href="https://static.igem.org/mediawiki/2012/b/bc/M4_recipe.pdf" target="_blank">M4 Recipe</a>
</li>
</li>
<li>
<li>
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<a href="#">Preparing Electrocompetent Cells</a>
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<a href="https://static.igem.org/mediawiki/2012/c/cb/Synthetic_River_Media.pdf" target="_blank">Synthetic River Media Recipe</a>
</li>
</li>
</ul>
</ul>

Latest revision as of 07:24, 2 October 2012

DNA Purification

DNA Assessment

PCR Methods

Plasmid Insertion

Plasmid Editing

Shewanella Growth

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