Team:Ehime-Japan

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         <li><a href="https://2012.igem.org/Team:Ehime-Japan">Home</a></li>  
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         <li><a href="https://2012.igem.org/Team:Ehime-Japan">Home</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Team">Team</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Project">Project</a>  
<li><a href="https://2012.igem.org/Team:Ehime-Japan/Project">Project</a>  
<ul>  
<ul>  
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<li><a href="">Overview</a></li>  
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#one">E.co-mail</a></li>  
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/human">Human Practise <li><a href="">G-nome Surfer Pro</a></li>  
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#two">E.co-Domino</a></li>
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<li><a href="">Primer</a></li>
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                        <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#three">E.cold-heat sensing system</a></li>  
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<li><a href="">Trumpet</a></li>
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#four">E.colingual</a></li>  
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<li><a href="">Puppetshow</a></li>  
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<li><a href="">Notebook</a></li>
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<li><a href="">Wet Lab</a></li>
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<li><a href="">i</a></li>
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<li><a href="#">Process</a>  
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        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Modeling">Modeling</a></li>
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<li><a href="#">Data</a>  
<ul>  
<ul>  
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<li><a href="">Methodology</a></li>  
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Parts">BioBrick parts</a></li>  
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<li><a href="">Safety</a></li>  
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/PlasmidsAndStrain">Plasmids And Strain</a></li>  
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<li><a href="">Notebook</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/PrimerList">Primer List</a></li>  
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<li><a href="">Outreach</a></li>  
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<li><a href="">Tips and Tricks</a></li>
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</ul>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/human">Human practise</a></li>  
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<li><a href="#">Human Practice</a>
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<li><a href="#">Additional Info</a>
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<ul>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create">E.create</a></li>  
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire">Questionnaire</a></li>
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</ul>
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<li><a href="#">Other</a>
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<ul>
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<li><a href="">Acknowledgement</a></li>  
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Team">team</a></li>  
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<li><a href="">Fun</a></li>
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<li><a href="https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan">team profile</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Notebook">note book</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Safety">safety</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Attributions">attribution</a></li>
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E.co-mail + E.co-Domino + E.cold-heat sensing system  =  E.colingual !!
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<br><img src="https://static.igem.org/mediawiki/2012/e/eb/Home%E3%81%AE%EF%BC%91.png" width=800px>
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→ Our projects
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    Ehime-Japan team tried three projects, and finally, we aims to  
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Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know <i>E. coli</i> feelings. It consists of three parts: Main, Connection, and Screen.<br>
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    create “E.colingual”, a tool through which we can know E.coli feelings.  
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To realize our project, we used the light sensor genes from <i>Synechocystis</i>, the Lon protease and the <i>ssrA</i> tag from <i>Mesoplasma florum</i>. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.
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    It consists of three parts: Main, Connection, and Screen.  
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    To realize our project, we used the light sensor genes from Synechocystis,  
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    Lon protease and ssrA tag from Mesoplasma florum. Also, a cell-cell signaling system  
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    and the heat shock and cold shock promoters were used so as to construct  
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    a foundational system of E.colingual.
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・E.co-mail (Connection)
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<img src="https://static.igem.org/mediawiki/2012/3/3d/Ecomail.png" width=800px>
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<img src="https://static.igem.org/mediawiki/2012/c/c8/E.comail.png" width=700px>
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    “E.co-mail” is a communication tool with E.coli and an optical fiber.  
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“E.co-mail” is a communication tool with <i>E. coli</i> and an optical fiber. We would be able to transfer digital information depicted in <i>E.coli</i> through a distance, and probably could send e-mails with this tool.   
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    We would be able to transfer digital information depicted in E.coli  
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    through a distance, and probably could send e-mails with this tool.   
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See more…
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a>
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・E.co-Domino (Screen)
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<img src="https://static.igem.org/mediawiki/2012/2/2d/Ecodomino_ehime_un.png" width=800px>
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     We tried to reconstruct toppling dominoes with E.coli,  
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<img src="https://static.igem.org/mediawiki/2012/9/9d/Eco-Domino.png" width=700px><br><br>
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     and we would be able to draw a movie of firework on LB plate!
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     We tried to reconstruct toppling dominoes with <i>E. coli</i>,  
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     and we would be able to draw a movie of firework on LB plate!  
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<br><img src="https://static.igem.org/mediawiki/2012/f/f4/%E2%91%A6.png"width="200px">
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See more…
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#two"><p><font color=blue>See more…</font color><p></a>
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・E.cold-heat sensing system (Main)
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<img src="https://static.igem.org/mediawiki/2012/a/a2/Sensing.png" width=800px><br>
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     Main is comprised of two different kinds of E.coli transformants.  
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     Main is comprised of two different kinds of <i>E. coli</i> transformants.  
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     One is that feels heat and cold respond differently, producing different
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     One is that feels heat and cold and respond differently, producing different
     signaling molecules. The other catches the signaling molecules and convert  
     signaling molecules. The other catches the signaling molecules and convert  
     the signal into the form of light. As a result, we can see a temperature change
     the signal into the form of light. As a result, we can see a temperature change
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     around the E.coli. The light signals could be transferred through a distance  
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     around the <i>E. coli</i>. The light signals could be transferred through a distance  
     and be displayed if the Connection and Screen systems work.
     and be displayed if the Connection and Screen systems work.
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See more…
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#three"><p><font color=blue>See more…</font color><p></a>
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・E.colingual
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     It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which  
     It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which  
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     we can know E.coli feelings:“hot”,“cold”, “happy” ,or “dying” etc…  
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     we can know <i>E. coli</i> feelings:“hot”,“cold”, “happy” ,or “dying” etc…  
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     Also, by an optical fiber, the information of those feelings are transmitted  
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     Also, by an optical fiber, the information of those feelings is transmitted  
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     to someone in a distant place from a lab. Actually, we focused on E.coli  
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     to someone in a distant place from a lab. Actually, we focused on <i>E. coli</i>
     feelings, “hot” and “cold”.  
     feelings, “hot” and “cold”.  
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See more...
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     We used two different ssrA tags to make a circulatable communication system. One is from
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     We used two different <i>ssrA</i> tags to make a circulatable communication system. One is from
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     M.florum recognized by the Lon protease from M.florum. The other is E.coli ssrA tag (LVA).
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     <i>M. florum</i> recognized by the Lon protease from <i>M. florum</i>. The other is <i>E. coli ssrA</i> tag (LVA).
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     Because the sequences of those tags are not similar, the degradation systems function
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     Because the sequences of those tags are not similar, the degradation systems should function
     independently.       
     independently.       
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a>
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     We made new BioBrick parts!
     We made new BioBrick parts!
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   ・BBa_  : This part is cold shock promoter + lasI.
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   ・BBa_K794000 : This part is cold shock promoter + <i>lasI</i>.
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<a href="https://2012.igem.org/Team:Ehime-Japan/Parts"><p><font color=blue>See more…</font color><p></a>
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・Ecreate !
・Ecreate !
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・Questionnaire
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Latest revision as of 03:52, 27 September 2012

Ehime-Japan iGEM Team: Welcome









Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know E. coli feelings. It consists of three parts: Main, Connection, and Screen.
To realize our project, we used the light sensor genes from Synechocystis, the Lon protease and the ssrA tag from Mesoplasma florum. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.








“E.co-mail” is a communication tool with E. coli and an optical fiber. We would be able to transfer digital information depicted in E.coli through a distance, and probably could send e-mails with this tool.


See more…










We tried to reconstruct toppling dominoes with E. coli, and we would be able to draw a movie of firework on LB plate!


See more…






Main is comprised of two different kinds of E. coli transformants. One is that feels heat and cold and respond differently, producing different signaling molecules. The other catches the signaling molecules and convert the signal into the form of light. As a result, we can see a temperature change around the E. coli. The light signals could be transferred through a distance and be displayed if the Connection and Screen systems work.


See more…










It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which we can know E. coli feelings:“hot”,“cold”, “happy” ,or “dying” etc… Also, by an optical fiber, the information of those feelings is transmitted to someone in a distant place from a lab. Actually, we focused on E. coli feelings, “hot” and “cold”.


See more…






→ New degradation system

We used two different ssrA tags to make a circulatable communication system. One is from M. florum recognized by the Lon protease from M. florum. The other is E. coli ssrA tag (LVA). Because the sequences of those tags are not similar, the degradation systems should function independently.


See more…






→ New parts

We made new BioBrick parts!
・BBa_K794000 : This part is cold shock promoter + lasI.


See more…






→ Human Practice

Ehime-Japan did a lot of projects as Human Practice!



・Ecreate !


We are enjoying gene recombination, and to tell interests of synthetic biology for high school students, we made “E.create”!! It is a card game that people can enjoy building genetic circuits. Also, we did educational activities for high school students and played them with E.create!


See more…


・Questionnaire

Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together! Thus, we were able to obtain a lot of replies, and found more general opinions about synthetic biology in Japan.


See more…









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Retrieved from "http://2012.igem.org/Team:Ehime-Japan"