Latest revision as of 18:36, 17 August 2012

Protocols

General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

Large Scale Double Digest

General Ligation Protocol

General Transformation Protocol

General Gel Purification Protocol

LB Plates (*includes instruction for liquid media)

Glycerol Stocks

SDS-PAGE gel electrophoresis

15% SDS-PAGE gel making protocol

Coomassie Staining of Acrylamide gels

Cell Lysis and Protein Purification Prep

Trypsin Digestion

Synechocystis sp. PCC6803 Protocols

Transformation Synechocystis 6803

Synechocystis 6803 media

Carotenoid Measurement Protocols

Carotenoid Extraction Using Ethanol

Zeaxanthin Extraction Using Hexane

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-{{WashUback}}
+__NOTOC__
+{{WashUbackprotocols}}
+<div id = "protocol">
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-<center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br>
+=<u>Protocols</u>=
+<div align = "left">
 =General Protocols=
-[https://2012.igem.org/Team:WashU/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
+[https://2012.igem.org/Team:WashU/Protocols/gel_electrophoresis General Agarose Gel Electrophoresis]
-[https://2012.igem.org/Team:WashU/Protocols/PCR  General PCR Protocol]
+[https://2012.igem.org/Team:WashU/Protocols/PCR General PCR Protocol]
-[https://2012.igem.org/Team:WashU/Protocols/Digestion  General Digestion Protocol]
+[https://2012.igem.org/Team:WashU/Protocols/Digestion General Digestion Protocol]
-[https://2012.igem.org/Team:WashU/Protocols/Ligation  General Ligation Protocol]
+[https://2012.igem.org/Team:WashU/Protocols/LargeDoubleDigestion#Large_Scale_Double_Digest Large Scale Double Digest]
-[https://2012.igem.org/Team:WashU/Protocols/Transformation  General Transformation Protocol]
+[https://2012.igem.org/Team:WashU/Protocols/Ligation General Ligation Protocol]
+[https://2012.igem.org/Team:WashU/Protocols/Transformation General Transformation Protocol]
 [https://2012.igem.org/Team:WashU/Protocols/GelPurification General Gel Purification Protocol]
+[https://2012.igem.org/Team:WashU/Protocols/LBPlates LB Plates (*includes instruction for liquid media)]
+[https://2012.igem.org/Team:WashU/Protocols/GlycerolStock Glycerol Stocks]
+[https://2012.igem.org/Team:WashU/Protocols/SDSPAGE SDS-PAGE gel electrophoresis]
+[https://2012.igem.org/Team:WashU/Protocols/SDSPAGE15 15% SDS-PAGE gel making protocol]
+[https://2012.igem.org/Team:WashU/Protocols/Commassie Coomassie Staining of Acrylamide gels]
+[https://2012.igem.org/Team:WashU/Protocols/Celllysis Cell Lysis and Protein Purification Prep]
+[https://2012.igem.org/Team:WashU/Protocols/Trypsin Trypsin Digestion]
 =Synechocystis sp. PCC6803 Protocols=
-[https://2012.igem.org/Team:WashU/Protocols/Transformation6803  Transformation Synechocystis 6803]
+[https://2012.igem.org/Team:WashU/Protocols/Transformation6803 Transformation Synechocystis 6803]
+[https://2012.igem.org/Team:WashU/Protocols/6803media Synechocystis 6803 media]
+=Carotenoid Measurement Protocols=
+[https://2012.igem.org/Team:WashU/Protocols/EthanolExtraction Carotenoid Extraction Using Ethanol]
-[https://2012.igem.org/Team:WashU/Protocols/6803media  Synechocystis 6803 media]
+[https://2012.igem.org/Team:WashU/Protocols/ZeaxanthinExtraction Zeaxanthin Extraction Using Hexane]<br>