Team:Exeter/lab book/proto

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     Competent Cells</u></a></li>
     Competent Cells</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Transferring Cultures to Liquid Medium</u></a></li>  
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Transferring Cultures to Liquid Medium</u></a></li>  
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     <li type="1" style="line-height:3"><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b"><u>Mini Prep</u></a></li>
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     <li type="1" style="line-height:3"><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b" target="_blank"><u>Mini Prep</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#1d1d1b"><u>New England BioLabs Biobrick Assembly Protocol</u></a>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#1d1d1b"><u>New England BioLabs Biobrick Assembly Protocol</u></a>
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     </li>
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     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/5" style="color:#1d1d1b"><u>Annealing Oligomer Primers</u></a></li>
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     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/5" style="color:#1d1d1b"><u>Annealing Oligomer Primers from Bioneer</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/6" style="color:#1d1d1b"><u>Biobrick Extraction</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/6" style="color:#1d1d1b"><u>Biobrick Extraction</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/7" style="color:#1d1d1b"><u>Two-Step PCR</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/7" style="color:#1d1d1b"><u>Two-Step PCR</u></a></li>
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     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/11" style="color:#1d1d1b"><u>Preparation of IDTE Buffer and IDT Re-Suspension</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/11" style="color:#1d1d1b"><u>Preparation of IDTE Buffer and IDT Re-Suspension</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/12" style="color:#1d1d1b"><u>3-Step PCR</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/12" style="color:#1d1d1b"><u>3-Step PCR</u></a></li>
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    <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/13" style="color:#1d1d1b"><u>SDS-PAGE Protocol</u></a></li>
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    <p><u>Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley</u> &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://igem.org/Team.cgi?id=764" style="color:#57B947" target="_blank"><u>Contact Us</u></a>  &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://2012.igem.org/Team:Exeter/site_map" style="color:#57B947"><u>Site Map</u></a></p>
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Latest revision as of 23:46, 26 September 2012

Protocol Home

Protocols
  • Transformation Protocol from Invitrogen, for Top10 Competent Cells
  • Transferring Cultures to Liquid Medium
  • Mini Prep
  • New England BioLabs Biobrick Assembly Protocol
  • Annealing Oligomer Primers from Bioneer
  • Biobrick Extraction
  • Two-Step PCR
  • Bacterial Genomic DNA Extraction Using GenElute
  • PCR purification using QIAquick
  • Linearized Plasmid Backbone Digestion and Ligation Protocol
  • Preparation of IDTE Buffer and IDT Re-Suspension
  • 3-Step PCR
  • SDS-PAGE Protocol
  • Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley   |   Contact Us   |   Site Map