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Team:Wageningen UR/Attributions
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== Team Achievements == | == Team Achievements == | ||
| - | All the work as described in the labjournals was executed by team | + | All the work as described in the labjournals was executed by team members of iGEM Wageningen_UR 2012. The table below highlights some of the special achievements of this team. A detailed description of the other work done can be found in the [[Team:Wageningen_UR/Journal|Journal]]. |
{| class="wikitable" style="text-align: left" | {| class="wikitable" style="text-align: left" | ||
| Line 16: | Line 16: | ||
|Done by | |Done by | ||
|Team member? | |Team member? | ||
| + | |requirement for | ||
|- | |- | ||
| - | |Measuring VLP size [[Team:Wageningen_UR/MethodsDetection#Dynamic_Light_Scattering_.28DLS.29|using DLS]] | + | |Measuring VLP size [[Team:Wageningen_UR/MethodsDetection#Dynamic_Light_Scattering_.28DLS.29|using DLS]] |
|Mark | |Mark | ||
|Yes | |Yes | ||
| + | |style="text-align:center;"|[[File:Medal_silver.png]] | ||
|- | |- | ||
| - | |Purification of VLPs using FPLC | + | |Purification of VLPs [[Team:Wageningen_UR/MethodsPurification|using FPLC]] |
|Mark | |Mark | ||
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
|Visualizing and measuring VLP size [[Team:Wageningen_UR/MethodsDetection|using EM]] | |Visualizing and measuring VLP size [[Team:Wageningen_UR/MethodsDetection|using EM]] | ||
|Mark, Wouter, Hugo, Kees | |Mark, Wouter, Hugo, Kees | ||
|Yes | |Yes | ||
| + | |style="text-align:center;"|[[File:Medal_silver.png]] | ||
|- | |- | ||
|Construction of [[Team:Wageningen_UR/InsideModification|inside]] and [[Team:Wageningen_UR/OutsideModification|outside]] modified VLPs | |Construction of [[Team:Wageningen_UR/InsideModification|inside]] and [[Team:Wageningen_UR/OutsideModification|outside]] modified VLPs | ||
| - | |Hugo, Jeroen | + | |Hugo, Jeroen, Robert |
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
| - | |Production of | + | |Production of [[Team:Wageningen_UR/MethodsDetection#EM_results|VLPs]] |
|All team members | |All team members | ||
|Yes | |Yes | ||
| + | |style="text-align:center;"|[[File:Medal_silver.png]] | ||
|- | |- | ||
|Writing the [[Team:Wageningen_UR/Protocol#Hepatitis_B_Coat_Protein_VLP_formation|protocol for HepBcAg VLPs]] | |Writing the [[Team:Wageningen_UR/Protocol#Hepatitis_B_Coat_Protein_VLP_formation|protocol for HepBcAg VLPs]] | ||
|Kees | |Kees | ||
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
| - | |Design and assembly of [[Team:Wageningen_UR/Coil_system#GFP_with_E-coil|GFP- | + | |Design and assembly of [[Team:Wageningen_UR/Coil_system#GFP_with_E-coil|GFP-E-coil construct]] |
| - | |Lisa | + | |Lisa, Kees, Robert |
|Yes | |Yes | ||
| + | |style="text-align:center;"|[[File:Medal_gold.png]] | ||
|- | |- | ||
|Modification of [[Team:Wageningen_UR/Coil_system#"leucine_zipper"_coils|existing BioBricks]] | |Modification of [[Team:Wageningen_UR/Coil_system#"leucine_zipper"_coils|existing BioBricks]] | ||
| - | |Robert | + | |Lisa, Robert |
|Yes | |Yes | ||
| + | |style="text-align:center;"|[[File:Medal_silver.png]] | ||
|- | |- | ||
| - | |Teaching about synthetic Biology on a [[Team:Wageningen_UR/Visiting_Secondary_School|secondary school]] | + | |Teaching about synthetic Biology on a [[Team:Wageningen_UR/Visiting_Secondary_School|secondary school]] |
|Joël, Niels | |Joël, Niels | ||
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
| - | |Organizing the participation of iGEM in the [[Team:Wageningen_UR/DiscoveryFestival|Discovery Festival]] | + | |Organizing the participation of iGEM in the [[Team:Wageningen_UR/DiscoveryFestival|Discovery Festival]] |
|Wouter and representatives of other Dutch iGEM teams | |Wouter and representatives of other Dutch iGEM teams | ||
|Partly | |Partly | ||
| + | |style="text-align:center;"|[[File:Medal_gold.png]] | ||
|- | |- | ||
| - | |[[Team:Wageningen_UR/Modeling| | + | |[[Team:Wageningen_UR/Modeling|Modeling]] of subunits and VLP formation |
| - | |Agneya, Thijs | + | |Agneya, Thijs, Kees |
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
|[[Team:Wageningen_UR/TheConstructor|The Constructor]] | |[[Team:Wageningen_UR/TheConstructor|The Constructor]] | ||
|Thijs, Jasper, iGEM Wageningen UR 2011 | |Thijs, Jasper, iGEM Wageningen UR 2011 | ||
|Partly | |Partly | ||
| + | |style="text-align:center;"|[[File:Medal_gold.png]][[File:Medal_software.png]] | ||
|- | |- | ||
|Isolation of the PLRV coat protein [[Team:Wageningen_UR/ObtainingthePoleroVLP|from natural samples]] | |Isolation of the PLRV coat protein [[Team:Wageningen_UR/ObtainingthePoleroVLP|from natural samples]] | ||
|Marnix | |Marnix | ||
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
|Presenting a poster at a [[Team:Wageningen_UR/CASconference|scientific conference]] | |Presenting a poster at a [[Team:Wageningen_UR/CASconference|scientific conference]] | ||
|All team members | |All team members | ||
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
|Organised a [[Team:Wageningen_UR/miniSymposium|mini symposium]] in the beginning of the competition for all dutch teams | |Organised a [[Team:Wageningen_UR/miniSymposium|mini symposium]] in the beginning of the competition for all dutch teams | ||
|Jeroen | |Jeroen | ||
|Yes | |Yes | ||
| + | | | ||
|- | |- | ||
| - | |[[Team:Wageningen_UR/Stakeholders| | + | |[[Team:Wageningen_UR/Stakeholders|Interviewed]] stakeholders about our project |
|Nick, Kees, Jeroen | |Nick, Kees, Jeroen | ||
|Yes | |Yes | ||
| + | | | ||
|} | |} | ||
| - | '' | + | ''Table 1. Overview of attributions and achievements.'' |
== Acknowledgements == | == Acknowledgements == | ||
| Line 87: | Line 104: | ||
With special thanks to: | With special thanks to: | ||
| - | Our supervisors Dr. Mark van Passel and Floor | + | Our supervisors Dr. Mark van Passel and Floor Hugenholtz, for asking the right questions and advising in problem solving |
| - | Dr. Richard Kormelink, for providing the templates of CCMV | + | Dr. Richard Kormelink, for providing the templates of CCMV and advising in VLP assembly |
Dr. Afshin Mehraban, for practical advice in working with VLPs | Dr. Afshin Mehraban, for practical advice in working with VLPs | ||
| + | |||
| + | Prof. Dr. George Lomonossoff and Dr. Hadrien Peyret for providing the HBcAg gene | ||
Dr. Jan van Lent, for giving team members a crash course in electron microscopy | Dr. Jan van Lent, for giving team members a crash course in electron microscopy | ||
Latest revision as of 02:50, 27 September 2012
Team Achievements
All the work as described in the labjournals was executed by team members of iGEM Wageningen_UR 2012. The table below highlights some of the special achievements of this team. A detailed description of the other work done can be found in the Journal.
| Activity | Done by | Team member? | requirement for |
| Measuring VLP size using DLS | Mark | Yes | 
|
| Purification of VLPs using FPLC | Mark | Yes | |
| Visualizing and measuring VLP size using EM | Mark, Wouter, Hugo, Kees | Yes |
|
| Construction of inside and outside modified VLPs | Hugo, Jeroen, Robert | Yes | |
| Production of VLPs | All team members | Yes |
|
| Writing the protocol for HepBcAg VLPs | Kees | Yes | |
| Design and assembly of GFP-E-coil construct | Lisa, Kees, Robert | Yes | 
|
| Modification of existing BioBricks | Lisa, Robert | Yes |
|
| Teaching about synthetic Biology on a secondary school | Joël, Niels | Yes | |
| Organizing the participation of iGEM in the Discovery Festival | Wouter and representatives of other Dutch iGEM teams | Partly |
|
| Modeling of subunits and VLP formation | Agneya, Thijs, Kees | Yes | |
| The Constructor | Thijs, Jasper, iGEM Wageningen UR 2011 | Partly | 
|
| Isolation of the PLRV coat protein from natural samples | Marnix | Yes | |
| Presenting a poster at a scientific conference | All team members | Yes | |
| Organised a mini symposium in the beginning of the competition for all dutch teams | Jeroen | Yes | |
| Interviewed stakeholders about our project | Nick, Kees, Jeroen | Yes |
Table 1. Overview of attributions and achievements.
Acknowledgements
With special thanks to:
Our supervisors Dr. Mark van Passel and Floor Hugenholtz, for asking the right questions and advising in problem solving
Dr. Richard Kormelink, for providing the templates of CCMV and advising in VLP assembly
Dr. Afshin Mehraban, for practical advice in working with VLPs
Prof. Dr. George Lomonossoff and Dr. Hadrien Peyret for providing the HBcAg gene
Dr. Jan van Lent, for giving team members a crash course in electron microscopy
Dr. Servé Kengen, for assistance and explanation about the FPLC
Ing. Remko Fokking, for explanation about DLS
The whole staff of Systems and Synthetic biology, Wageningen UR, for the lab space and tolerating our presence.
Our sponsors
