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Team:Wageningen UR/Protocol
From 2012.igem.org
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<li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li> | <li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li> | <li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/FormationBufferPolero|Formation Buffer Polero]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li> | <li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li> | <li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li> | ||
| Line 39: | Line 40: | ||
<li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li> | <li>[[Team:Wageningen_UR/Protocol/StartupCCMV|Growing culture]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li> | <li>[[Team:Wageningen_UR/Protocol/DialysisCCMV|Dialysis of the VLPs]]</li> | ||
| - | <li>[[Team:Wageningen_UR/Protocol/RoundupCCMV| | + | <li>[[Team:Wageningen_UR/Protocol/RoundupCCMV|Purifying the VLPs]]</li> |
</ul> | </ul> | ||
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<li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li> | <li>[[Team:Wageningen_UR/Protocol/StartupHepB|Growing culture]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li> | <li>[[Team:Wageningen_UR/Protocol/DialysisHepB|Dialysis of the VLPs]]</li> | ||
| - | <li>[[Team:Wageningen_UR/Protocol/RoundupHepB| | + | <li>[[Team:Wageningen_UR/Protocol/RoundupHepB|Purifying the VLPs]]</li> |
</ul> | </ul> | ||
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<ul> | <ul> | ||
<li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li> | <li>[[Team:Wageningen_UR/Protocol/RNA|RNA isolation from potato leaf material]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/StartupPolero|Growing culture]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/DialysisPolero|Dialysis of the VLPs]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/RoundupPolero|Purifying the VLPs]]</li> | ||
</ul> | </ul> | ||
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<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li> | <li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li> | <li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/Mutagenesis|Mutagenesis]]</li> | ||
</ul> | </ul> | ||
Latest revision as of 10:44, 26 October 2012
Contents |
Methods
The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.
Protocols
Medium & Buffer recipes
- LB medium
- SOB medium
- SOC medium
- Disassembly buffer
- Dialysis buffer 10x
- Reassembly buffer
- Virus buffer
- Formation Buffer HepBcAg
- Washing Buffer HepBcAg
- Formation Buffer Polero
- Towbin's electrotransfer buffer 1x
- Towbin's electrotransfer buffer 10x
CCMV Coat Protein VLP formation
Hepatitis B Coat Protein VLP formation
Polerovirus Coat Protein VLP formation
General Protocol
- Preparation of electrocompetent cells of E. coli
- SDS-Polyacrylamide and native gel electrophoresis
- Dynamic Light Scattering user manual
- French press user manual
- FPLC user manual
- Mutagenesis
