Team:Macquarie Australia/Protocols/SDSPAGE

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<center><h1>SDS-PAGE</h1></center>
<center><h1>SDS-PAGE</h1></center>
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<br>
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<center>Pelleted cells were resuspended in 200 uL Milli-Q H2O. </center> <br>
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<center>Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. </center><br>
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<center>Using a Hamilton syringe, the cells were sheared. </center><br>
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<center>Centrifuged the preparations for 3 mins @ 13,000 rpm.</center> <br>
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<center>Loaded 20 uL of the supernatant in to the gel. </center> <br>
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<center>Electrophoresis was conducted at constant voltage (200 V) for one hour. </center> <br>

Latest revision as of 03:54, 27 September 2012



SDS-PAGE


Pelleted cells were resuspended in 200 uL Milli-Q H2O.

Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.

Using a Hamilton syringe, the cells were sheared.

Centrifuged the preparations for 3 mins @ 13,000 rpm.

Loaded 20 uL of the supernatant in to the gel.

Electrophoresis was conducted at constant voltage (200 V) for one hour.