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From 2012.igem.org

Revision as of 19:50, 23 September 2012 (view source) Machat (Talk | contribs) ← Older edit		Latest revision as of 15:17, 21 October 2012 (view source) Machat (Talk | contribs)
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	<br/>		<br/>
	<br/>		<br/>
		+	<a name="alas"></a>
	<center>		<center>
	<img src="https://static.igem.org/mediawiki/2012/7/78/Tad51.png" WIDTH=850 HEIGHT=150 alt="tadpole volume computation" />		<img src="https://static.igem.org/mediawiki/2012/7/78/Tad51.png" WIDTH=850 HEIGHT=150 alt="tadpole volume computation" />
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	<img src="https://static.igem.org/mediawiki/2012/9/96/Tail-volume.png" alt="tail volume computation" />		<img src="https://static.igem.org/mediawiki/2012/9/96/Tail-volume.png" alt="tail volume computation" />
	</center>		</center>
-		+	<a name="attlas"></a>
	<h2>Volume of the head</h2>		<h2>Volume of the head</h2>
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	<img src="https://static.igem.org/mediawiki/2012/2/27/Head-volume.png" alt="head volume computation" />		<img src="https://static.igem.org/mediawiki/2012/2/27/Head-volume.png" alt="head volume computation" />
	</center>		</center>
-		+	<a name="atttlas"></a>
	<h2>Volume of the tadpole</h2>		<h2>Volume of the tadpole</h2>
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	</center>		</center>
-		+	<a name="attttlas"></a>
	<h2>Volume of the skin</h2>		<h2>Volume of the skin</h2>
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	First the volume for the skin in the head:		First the volume for the skin in the head:
	<center>		<center>
-	<img src="https://static.igem.org/mediawiki/2012/6/6a/Head-int-volume.png" alt="head skin volume computation" />	+	<img src="https://static.igem.org/mediawiki/2012/6/60/Vblo.png" alt="head skin volume computation" />
	</center>		</center>
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	<img src="https://static.igem.org/mediawiki/2012/0/0e/Skin-volume.png" alt="skin volume computation" />		<img src="https://static.igem.org/mediawiki/2012/0/0e/Skin-volume.png" alt="skin volume computation" />
	</center>		</center>
-		+	<a name="atttttlas"></a>
	<h2>Volume of the blood</h2>		<h2>Volume of the blood</h2>
-	<p>According to a study published in the article "THE BLOOD-VOLUME OF COLD-BLOODED ANIMALS AS DETERMINED BY EXPERIMENTS UPON FROGS AND LIZARDS", the average volume of blood in a frog in cubic centimetre is equal to 4.61% of its masse in grammes.	+	<p>According to a study published in the article "The Blood-Volume of Cold-Blooded Animals as Determined by Experiments Upon Frogs and Lizards", the average volume of blood in a frog in cubic centimetre is equal to 4.61% of its masse in grammes.
	We took the same percentage for a tadpole. </br>		We took the same percentage for a tadpole. </br>
-	For the masse, we estimated a value m<sub>g</sub>=0.5grammes from an "Amphibian Metamorphosis Assay Integrated Summary Report" where the wet weight of a 51th-stage-tadpole was studied under chemical substances effects. <p/>	+	For the masse, we mesured m<sub>g</sub>=1mg for a wet-weighed 5-days tadpole. <p/>
	<center>		<center>
-	<img src="https://static.igem.org/mediawiki/2012/6/60/Vblo.png" alt="tail volume computation" />	+	<img src="https://static.igem.org/mediawiki/2012/e/e4/Ta7.png" alt="tail volume computation" />
	</center>		</center>
	<h2>Volume of the receptor</h2>		<h2>Volume of the receptor</h2>
	We considered the receptor as any tissue which can recept the auxin via the blood except the skin.		We considered the receptor as any tissue which can recept the auxin via the blood except the skin.
	<center>		<center>
-	<img src="https://static.igem.org/mediawiki/2012/6/60/Vblo.png" alt="tail volume computation" />	+	<img src="https://static.igem.org/mediawiki/2012/7/7f/Ta11.png" alt="tail volume computation" />
	</center>		</center>
	<script type="text/javascript">writeFooter()</script>		<script type="text/javascript">writeFooter()</script>
	</html>		</html>

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Latest revision as of 15:17, 21 October 2012

Tadpole's volume calculation

The tadpole shape is approximated by a cone and a sphere.

Volume of the tail

r_b = 0.035cm
H = 0.8cm

Volume of the head

r_h = 0.3cm

Volume of the tadpole

Volume of the skin

To compute the volume of the skin we do the difference between the external and internal sphere/cone. T_skin = 35 * 10^-4 cm First the volume for the skin in the head: Then the volume for the skin in the tail: Finally the whole tadpole skin volume:

Volume of the blood

According to a study published in the article "The Blood-Volume of Cold-Blooded Animals as Determined by Experiments Upon Frogs and Lizards", the average volume of blood in a frog in cubic centimetre is equal to 4.61% of its masse in grammes. We took the same percentage for a tadpole.
For the masse, we mesured m_g=1mg for a wet-weighed 5-days tadpole.

Volume of the receptor

We considered the receptor as any tissue which can recept the auxin via the blood except the skin.