Team:TU Darmstadt/Materials/LB Tributyrin

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Latest revision as of 20:37, 26 September 2012

LB-Agar-Tributyrin

About

Modification of LB plates for hydrolytic activity detection. Tributyrin serves as a model substrate and is a good indicator for hydrolytic activity.

Tributyrin.png

Ingredients

  • 5 g tryptone
  • 10 g yeast extract
  • 5 g NaCl
  • 15 g agar-agar
  • 15 mL Tributyrin medium
    • 5 g gum arabic
    • 5 mL Tributyrin
    • ad. 15 mL dist. water
    • sonification for 45 min.
  • ad. 1 L dist. water
  • autoclave for 20 min. at 121°C
  • wait until the temperature decreased under 60°C and add the prefered antibiotic
  • pour the medium into petri plates (1 L LB-Agar is equal to 40 plates)
  • store the plates at 4°C