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| - | <h1>Orthogonal hormonal system</h1>
| + | moved to https://2012.igem.org/Team:Evry/AIDSystem |
| - | <p>Our orthogonal hormonal system is based on the use of auxin, a plant hormone. To implement this system, we used two different type of device corresponding to the production and the reception of auxin in the tadpole.</p>
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| - | <h2>Auxin production devices</h2>
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| - | <h3>Pathway</h3>
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| - | <center>
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| - | <img src="https://static.igem.org/mediawiki/2012/7/71/AuxinPathway.jpg" width="700px" alt="Auxin pathway" />
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| - | </center>
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| - | <h3>Devices</h3>
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| - | <p>We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.</p>
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| - | <center>
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| - | <img src="https://static.igem.org/mediawiki/2012/b/ba/Production3devices.jpg" width="600px" alt="3 devices for production" />
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| - | </center>
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| - | <ul>
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| - | <li> Auxin production device 1 : this device is composed of BBa_K812???, coding for IaaM, and BBa_K812120
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| - | , coding for IaaH (Indoleacetamide hydrolyase). for auxin generator, with a kozak sequence for the use in tadpole's embryos.
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| - | <li> Auxin production device 2 : this device is composed of BBa_K812014. It is ment for the co-expression of IaaH and IaaM gene in the same cell in tadpole.
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| - | This two devices have been designed to be expressed in
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| - | <li> Auxin production device 3 : this device is composed of BBa_K515100, coding for IaaM and IaaH.
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| - | <br>
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| - | <li> Auxin reception device 1 : this device is composed of 2 plasmids, one containing : BBa_K812010 coding for GFP-AID, and, the other by BBa_K812012 coding for OsTir1.
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| - | <li> Auxin reception device 2 : this device is composed of BBa_K812013 coding for GFP-AID and OsTir1 in the same cell.
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| - | <center>
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| - | <img src="https://static.igem.org/mediawiki/2012/a/a7/DeviceProdbis.jpg" width="900px" alt="Production devices" />
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| - | </center>
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| - | <h2>Auxin reception device</h2>
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