Team:Evry/OrthogonalSyst

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<h1>Orthogonal hormonal system</h1>
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<p>Our orthogonal hormonal system is based on the use of auxin, a plant hormone. To implement this system, we used two different type of device corresponding to the production and the reception of auxin in the tadpole.</p>
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<h2>Auxin production devices</h2>
 
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<h3>Pathway</h3>
 
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<img src="https://static.igem.org/mediawiki/2012/7/71/AuxinPathway.jpg" width="700px" alt="Auxin pathway" />
 
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<h3>Devices</h3>
 
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<p>We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.</p>
 
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<img src="https://static.igem.org/mediawiki/2012/b/ba/Production3devices.jpg" width="600px" alt="3 devices for production" />
 
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<ul>
 
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<li> Auxin production device 1 : this device is composed of BBa_K812???, coding for IaaM, and BBa_K812120
 
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, coding for IaaH (Indoleacetamide hydrolyase). for auxin generator, with a kozak sequence for the use in tadpole's embryos.
 
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<li> Auxin production device 2 : this device is composed of BBa_K812014. It is ment for the co-expression of IaaH and IaaM gene in the same cell in tadpole.
 
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This two devices have been designed to be expressed in
 
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<li> Auxin production device 3 : this device is composed of BBa_K515100, coding for IaaM and IaaH.
 
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<center>
 
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<img src="https://static.igem.org/mediawiki/2012/5/54/DeviceProd.jpg" width="900px" alt="2 devices" />
 
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<h2>Auxin reception device</h2>
 
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Latest revision as of 20:51, 23 September 2012

moved to https://2012.igem.org/Team:Evry/AIDSystem