"
Team:Tuebingen/NotebookAppendix
From 2012.igem.org
(Difference between revisions)
(→Constructs) |
(→Software) |
||
| (9 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:Tuebingen/Template/Tuebingen}} | {{:Team:Tuebingen/Template/Tuebingen}} | ||
| - | + | = Appendix = | |
''To complete this report we list all products and software used over the course of our project.'' | ''To complete this report we list all products and software used over the course of our project.'' | ||
| - | + | == Constructs == | |
| - | + | <table class="wikitable"> | |
| - | + | <tr><th>backbone pRS#</th><th>part #1</th><th>part #2</th><th>part #3</th></tr> | |
| - | + | <tr><td>313</td><td>Padh1</td><td>mPR ''Danio rerio''</td><td>Tadh1</td></tr> | |
| - | + | <tr><td>313</td><td>Padh1</td><td>mPR ''Xenopus laevis''</td><td>Tadh1</td></tr> | |
| - | + | ||
| - | <table | + | |
| - | <tr><th>pRS</th><th> | + | |
| - | <tr><td>313</td><td>Padh1</td><td>mPR | + | |
| - | <tr><td>313</td><td>Padh1</td><td>mPR Xenopus laevis</td><td>Tadh1</td></tr> | + | |
<tr><td>315</td><td>Pfet3</td><td>rox1</td><td>Tadh1</td></tr> | <tr><td>315</td><td>Pfet3</td><td>rox1</td><td>Tadh1</td></tr> | ||
<tr><td>315</td><td>Pfet3</td><td>mig1</td><td>Tadh1</td></tr> | <tr><td>315</td><td>Pfet3</td><td>mig1</td><td>Tadh1</td></tr> | ||
| Line 23: | Line 18: | ||
</table> | </table> | ||
| - | + | == Chemicals == | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
We needed the following chemicals: | We needed the following chemicals: | ||
| Line 60: | Line 36: | ||
* Agar-Agar | * Agar-Agar | ||
| - | + | == Software == | |
| - | + | ||
The following list of software was used in the team: | The following list of software was used in the team: | ||
| Line 70: | Line 45: | ||
* [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] <br/> BLAST was our main sequence search tool. It was quite useful for controlling sequence results. | * [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] <br/> BLAST was our main sequence search tool. It was quite useful for controlling sequence results. | ||
| - | * [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE] | + | * [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE] <br/> The free and useful plasmid editor. |
* [http://drive.google.com Google Drive] <br/> Google Drive was used for our documentation and all of our collaborative work (papers, poster, images). | * [http://drive.google.com Google Drive] <br/> Google Drive was used for our documentation and all of our collaborative work (papers, poster, images). | ||
Latest revision as of 12:53, 26 September 2012
Contents |
Appendix
To complete this report we list all products and software used over the course of our project.
Constructs
| backbone pRS# | part #1 | part #2 | part #3 |
|---|---|---|---|
| 313 | Padh1 | mPR Danio rerio | Tadh1 |
| 313 | Padh1 | mPR Xenopus laevis | Tadh1 |
| 315 | Pfet3 | rox1 | Tadh1 |
| 315 | Pfet3 | mig1 | Tadh1 |
| 316 | Panb1 | lacZ | Tadh1 |
| 316 | Panb1 | luciferase | Tadh1 |
| 316 | Psuc2 | lacZ | Tadh1 |
| 316 | Psuc2 | luciferase | Tadh1 |
Chemicals
We needed the following chemicals:
- Ampicillin
beta-lactam antibiotic - Agarose
Polysaccharide, major component of Agar - Dimethylsulfoxid (DMSO)
organic solvent - Acetic Acid
- Ethylenediaminetetraacetic acid (EDTA)
- Ethanol
- TRIS
buffer solution for enzymes - Nucleoside triphosphate
- Trypton
- Isopropanol
- Isopropyl-β-D-thiogalactopyranosid (IPTG)
- LB-medium
used for growth of E.coli - Agar-Agar
Software
The following list of software was used in the team:
- [http://ugene.unipro.ru/ Unipro UGENE]
UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
- [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
All our primers were designed using Vector NTI which is also used by our advisors.
- [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
- [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
The free and useful plasmid editor.
- [http://drive.google.com Google Drive]
Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).
