Team:Tuebingen/NotebookAppendix

From 2012.igem.org

(Difference between revisions)
(→Constructs)
(→Software)
 
(12 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Tuebingen/Template/Tuebingen}}
{{:Team:Tuebingen/Template/Tuebingen}}
-
== Appendix ==
+
= Appendix =
''To complete this report we list all products and software used over the course of our project.''
''To complete this report we list all products and software used over the course of our project.''
-
=== Constructs ===
+
== Constructs ==
-
<!--
+
<table class="wikitable">
-
pRS mit jeweiligen Inserts
+
<tr><th>backbone pRS#</th><th>part #1</th><th>part #2</th><th>part #3</th></tr>
-
-->
+
<tr><td>313</td><td>Padh1</td><td>mPR ''Danio rerio''</td><td>Tadh1</td></tr>
-
 
+
<tr><td>313</td><td>Padh1</td><td>mPR ''Xenopus laevis''</td><td>Tadh1</td></tr>
-
 
+
<tr><td>315</td><td>Pfet3</td><td>rox1</td><td>Tadh1</td></tr>
-
<table border="solid">
+
<tr><td>315</td><td>Pfet3</td><td>mig1</td><td>Tadh1</td></tr>
-
<tr><th>pRS</th><th>p1</th><th>p2</th><th>p3</th></tr>
+
<tr><td>316</td><td>Panb1</td><td>lacZ</td><td>Tadh1</td></tr>
-
<tr><th>313</th><th>Padh1</th><th>mPR danio rerio</th><th>Tadh1</th></tr>
+
<tr><td>316</td><td>Panb1</td><td>luciferase</td><td>Tadh1</td></tr>
-
<tr><th>313</th><th>Padh1</th><th>mPR Xenopus laevis</th><th>Tadh1</th></tr>
+
<tr><td>316</td><td>Psuc2</td><td>lacZ</td><td>Tadh1</td></tr>
-
<tr><th>315</th><th>Pfet3</th><th>rox1</th><th>Tadh1</th></tr>
+
<tr><td>316</td><td>Psuc2</td><td>luciferase</td><td>Tadh1</td></tr>
-
<tr><th>315</th><th>Pfet3</th><th>mig1</th><th>Tadh1</th></tr>
+
-
<tr><th>316</th><th>Panb1</th><th>lacZ</th><th>Tadh1</th></tr>
+
-
<tr><th>316</th><th>Panb1</th><th>luciferase</th><th>Tadh1</th></tr>
+
-
<tr><th>316</th><th>Psuc2</th><th>lacZ</th><th>Tadh1</th></tr>
+
-
<tr><th>316</th><th>Psuc2</th><th>luciferase</th><th>Tadh1</th></tr>
+
</table>
</table>
-
=== Protocols ===
+
== Chemicals ==
-
 
+
-
<!--
+
-
* RV
+
-
* Ligation
+
-
* Transformation
+
-
* komp. Zellen
+
-
* PCR mit Temp.
+
-
-->
+
-
 
+
-
 
+
-
=== Kits ===
+
-
 
+
-
* Genaxxon Plasmid DNA Purification Mini Prep
+
-
* Genaxxon Gel Extraction Mini Prep
+
-
* Genaxxon PCR DNA Purification Mini Prep
+
-
* Qiagen Plasmid Midi
+
-
 
+
-
 
+
-
=== Chemicals ===
+
We needed the following chemicals:
We needed the following chemicals:
Line 60: Line 36:
* Agar-Agar
* Agar-Agar
-
 
+
== Software ==
-
=== Software ===
+
The following list of software was used in the team:
The following list of software was used in the team:
Line 70: Line 45:
* [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] <br/> BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
* [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] <br/> BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
-
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
+
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE] <br/> The free and useful plasmid editor.
* [http://drive.google.com Google Drive] <br/> Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).
* [http://drive.google.com Google Drive] <br/> Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).

Latest revision as of 12:53, 26 September 2012



Contents

Appendix

To complete this report we list all products and software used over the course of our project.

Constructs

backbone pRS#part #1part #2part #3
313Padh1mPR Danio rerioTadh1
313Padh1mPR Xenopus laevisTadh1
315Pfet3rox1Tadh1
315Pfet3mig1Tadh1
316Panb1lacZTadh1
316Panb1luciferaseTadh1
316Psuc2lacZTadh1
316Psuc2luciferaseTadh1

Chemicals

We needed the following chemicals:

  • Ampicillin
    beta-lactam antibiotic
  • Agarose
    Polysaccharide, major component of Agar
  • Dimethylsulfoxid (DMSO)
    organic solvent
  • Acetic Acid
  • Ethylenediaminetetraacetic acid (EDTA)
  • Ethanol
  • TRIS
    buffer solution for enzymes
  • Nucleoside triphosphate
  • Trypton
  • Isopropanol
  • Isopropyl-β-D-thiogalactopyranosid (IPTG)
  • LB-medium
    used for growth of E.coli
  • Agar-Agar

Software

The following list of software was used in the team:

  • [http://ugene.unipro.ru/ Unipro UGENE]
    UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
  • [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
    All our primers were designed using Vector NTI which is also used by our advisors.
  • [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
    BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
  • [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
    The free and useful plasmid editor.
  • [http://drive.google.com Google Drive]
    Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).
Retrieved from "http://2012.igem.org/Team:Tuebingen/NotebookAppendix"