Team:Evry/Achievements

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<h1>iGEM Evry 2012 achievements</h1>
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<p>Asides from having lots of fun, meeting great people, gathering around cheese and wine and lazying around the Genopole gardens during lunch break, we also did a little bit of work. Here is a list of our achievements:</p><br>
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<center><h2><a href="https://2012.igem.org/Team:Evry/FrenchFrog"><img src="https://static.igem.org/mediawiki/2012/b/bd/Xenope.png" alt="" width="200px"></a></h2></center>
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<li>We brought a new multicellular <a href="https://2012.igem.org/Team:Evry/FrenchFrog">chassis</a> to iGEM</li>
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<li>We submitted <a href="https://2012.igem.org/Team:Evry/Parts">21 biobricks</a> to the registry, including the first working Xenopus biobricks</li>
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<li>We characterized 12 Biobricks:
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812050">BBa_K812050</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812031">BBa_K81203</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812032">BBa_K812032</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812110">BBa_K812110</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812130">BBa_K812130</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812132">BBa_K812132</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812133">BBa_K812133</a>,
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812200">BBa_K812200</a> and
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812233">BBa_K812233</a>.</li>
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<li>We designed a method for testing multicellular systems in <a href="https://2012.igem.org/Team:Evry/FrenchFrog"><i>Xenopus</i></a> rapidly</li>
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<li>We provided 3 new plasmids for using this method, which include debugging tools, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812200">BBa_K812200</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812300">BBa_K812300</a></li>
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<li>Our reporters and promoters were <a href="https://2012.igem.org/Team:Evry/Tadpole_injection1">characterized</a>.</li>
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<li>We did a collaboration with Team Slovenia and we injected their construction into our fertilized embryos: <a href="https://2012.igem.org/Team:Evry/TeamSlovenia_collaboration">Results</a></li>
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</ul>
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<center><h2><a href="https://2012.igem.org/Team:Evry/AIDSystem"><img src="https://static.igem.org/mediawiki/2012/e/e2/Hormone.png" width="200px"></a></h2></center>
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<li>We designed a <a href="https://2012.igem.org/Team:Evry/AIDSystem">multicellular hormonal</a> system</li>
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<li>We created an Auxin production (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812014">BBa_K812014</a>) device for use in eukaryotes from the prokaryote one</li>
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<li>We submitted the Tir1 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812012">BBa_K812012</a>)  auxin detection device to the registry</li>
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<li>We showed the first <a href="https://2012.igem.org/Team:Evry/BXcom">Synthetic ecosystem</a>, with a unicellular chassis (<i>E.coli</i>) improved inside a multicellular chassis (<i>Xenopus</i>)</li>
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</ul>
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<center><h2><a href="https://2012.igem.org/Team:Evry/GB"><img src="https://static.igem.org/mediawiki/2012/a/a4/GoldeN.png" width="200px"></a></h2></center>
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<li>We designed a new <a href="https://2012.igem.org/Team:Evry/GB#requirement">biobrick standard</a which allows one-shot coding device assembly, while still being compatible with the RFC10 standard</li>
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<li>We demonstrated it <a href="https://2012.igem.org/Team:Evry/GB#GB">works</a> (still with a low efficiency) and that the protocol have to be further optimized</li>
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<li>We <a href="https://2012.igem.org/Team:Evry/GB#GG">managed</a> to assemble parts with a very high efficiency using Golden Gate and the GoldenBricks plasmids</li>
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</ul>
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<center><h2><a href="https://2012.igem.org/Team:Evry/Modeling"><img src="https://static.igem.org/mediawiki/2012/5/5b/ModelingDHIUHEIUHD.png" width="200px"></a></h2></center>
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<li>We used a <a href="https://2012.igem.org/Team:Evry/Modeling">multi-level approach</a> to model various aspects of the <a href="https://2012.igem.org/Team:Evry/Auxin_diffusion">physiology</a> and <a href="https://2012.igem.org/Team:Evry/auxin_production">cellular behaviour</a> of Xenopus.</li>
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<li>All our models <a href="https://2012.igem.org/Team:Evry/model_integration">fit together</a> to comprehensively depict all the processes from the experimental protocol to <a href="https://2012.igem.org/Team:Evry/auxin_detection">reporter expression</a></li>
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<li>We used many modelisation techniques (<a href="https://2012.igem.org/Team:Evry/ODE_model">Ordinary differential equations</a>, <a href="https://2012.igem.org/Team:Evry/auxin_pde">some derived</a> from partial differential equations and <a href="https://2012.igem.org/Team:Evry/plasmid_splitting">Gillespie algorithm</a>) in order to benefit from their respective strength.</li>
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</ul>
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<center><h2><a href="https://2012.igem.org/Team:Evry/HumanPractice"><img src="https://static.igem.org/mediawiki/2012/b/ba/HumanPractice.png" width="200px"></a></h2></center>
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<li>We submitted an <a href="https://2012.igem.org/Team:Evry/HumanPractice">extensive report</a> of our conclusions about synthetic biology in vertebrates, which is the fruit of our long reflection we conducted over the summer.</li>
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Latest revision as of 03:24, 27 October 2012

iGEM Evry 2012 achievements


Asides from having lots of fun, meeting great people, gathering around cheese and wine and lazying around the Genopole gardens during lunch break, we also did a little bit of work. Here is a list of our achievements:



  • We designed a multicellular hormonal system
  • We created an Auxin production (BBa_K812014) device for use in eukaryotes from the prokaryote one
  • We submitted the Tir1 (BBa_K812012) auxin detection device to the registry
  • We showed the first Synthetic ecosystem, with a unicellular chassis (E.coli) improved inside a multicellular chassis (Xenopus)

  • We designed a new biobrick standard
  • We demonstrated it works (still with a low efficiency) and that the protocol have to be further optimized
  • We managed to assemble parts with a very high efficiency using Golden Gate and the GoldenBricks plasmids

  • We submitted an extensive report of our conclusions about synthetic biology in vertebrates, which is the fruit of our long reflection we conducted over the summer.

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