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Team:TU Darmstadt/Protocols/Glycerine stock
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<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
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In order to have a permanent culture of cells glycerine stocks can be made. | In order to have a permanent culture of cells glycerine stocks can be made. | ||
=== Protocol === | === Protocol === | ||
| - | * Add 200 µl of sterilized glycerine to 800 µl cell culture and mix well | + | * Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well |
* Freeze the stock at -20 °C | * Freeze the stock at -20 °C | ||
Latest revision as of 21:16, 26 September 2012
Glycerine stock
In order to have a permanent culture of cells glycerine stocks can be made.
Protocol
- Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
- Freeze the stock at -20 °C
